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4.98.1.1: protoporphyrin ferrochelatase

This is an abbreviated version!
For detailed information about protoporphyrin ferrochelatase, go to the full flat file.

Word Map on EC 4.98.1.1

Reaction

protoheme
+ 2 H+ =
protoporphyrin
 +
Fe2+

Synonyms

chelatase, ferro-, EC 4.99.1.1, FC1, FC2, FeC, FeCH, ferro-protoporphyrin chelatase, ferrochelatase, ferrochelatase 1, ferrochelatase I, ferrochelatase II, frataxin, HEM15, heme synthase, heme synthetase, hemH, HemH1, HemH2, hFC, host red cell ferrochelatase, iron chelatase, parasite genome-coded ferrochelatase, PfFC, PPIX ferrochelatase, protohaem ferrolyase, protoheme ferro-lyase, protoheme ferrolyase, protoheme lyase, protoporhyrin IX ferrochelatase, protoporphyrin (IX) ferrochelatase, protoporphyrin IX ferrochelatase, Shew_1140, Shew_2229, type II ferrochelatase

ECTree

     4 Lyases
         4.98 ATP-independent chelatases
             4.98.1 Forming coordination complexes
                4.98.1.1 protoporphyrin ferrochelatase

Purification

Purification on EC 4.98.1.1 - protoporphyrin ferrochelatase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
anti-FLAG M2 affinity gel column chromatography or Ni2+-charged His-select resin column chromatography
-
by gel filtration
by metal affinity chromatography
chromatography
-
complex between protoporphyrinogen dehydrogenase PgdH1 and protoporphyrin ferrochelatase PpfC, recombinant protein
-
expressed in Escherichia coli
five-step purification, by gel filtration, purified about 410fold with a 3% yield
-
immunopurification from mitochondrial extracts
Ni-IMAC column chromatography and Sephacryl S-300 gel filtration
Ni-NTA column chromatography. After aerobic purification, the cysteine residues can oxidize and release the [2Fe-2S] cluster, causing ferrochelatase to precipitate. CHAPS is best at solubilizing ferrochelatase, while retaining full activity as compared to the enzyme in sodium cholate
Ni2+-NTA bead chromatography
Ni2+-NTA bead column chromatography, gel filtration
-
precipitates immediately after purification, making it unsuitable for further analysis
recombinant enzyme, expressed in Escherichia coli
-
recombinant protein
recombinant wild-type and mutants P255R, S249A/K250Q/V251C, K250M/V251L/W256Y, Q248P/S249G/K250P/G252W
the recombinant enzyme
wild-type and deltaH324 mutant, by affinity chromatography and gel filtration
-
with Ni2+ beads
-