Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | b5 reductase required for the full enzyme activity | Mus musculus | |
additional information | recombinant ferrochelatase exhibits iron removal activity when added together with purified recombinant b5 reductase | Homo sapiens |
Application | Comment | Organism |
---|---|---|
additional information | reverse reaction of ferrochelatase, which may contribute to a new route of the recycling of protoporphyrin and heme in cells | Mus musculus |
additional information | reverse reaction of ferrochelatase, which may contribute to a new route of the recycling of protoporphyrin and heme in cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
Escherichia coli expressing His-tagged ferrochelatase | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C395delta | deletion of the C-terminus of the enzyme, where the iron-sulfur is located, results in the loss of both ferrochelatase activity and iron-removal activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Mus musculus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Homo sapiens | |
Co2+ | - |
Homo sapiens | |
Fe2+ | - |
Mus musculus | |
Fe2+ | - |
Homo sapiens | |
Sn2+ | - |
Homo sapiens | |
Zn2+ | - |
Mus musculus | |
Zn2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
five-step purification, by gel filtration, purified about 410fold with a 3% yield | Mus musculus |
with Ni2+ beads | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
BALB/3T3 cell | conversion of mesoporphyrin is dependent on the expression of ferrochelatase, since control Balb/3T3 cells have much lower expression of ferrochelatase than the ferrochelatase transfectants | Mus musculus | - |
liver | - |
Mus musculus | - |
additional information | M-TAT cell, when induced to undergo erythroid differentiation M-TAT cells convert more zinc mesoporphyrin than control cells, accompanied by an increase in hemoglobin-synthesizing cells | Homo sapiens | - |
additional information | MEL cell, clear conversion of zinc mesoporphyrin, marked increase in the conversion in erythroid differentiation-induced MEL cells, in which ferrochelatase is induced | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cd2+-protoporphyrin + H+ | poor substrate | Homo sapiens | protoporphyrin + Cd2+ | - |
? | |
Co2+-protoporphyrin + H+ | - |
Homo sapiens | protoporphyrin + Co2+ | - |
? | |
Fe2+-mesoporphyrin IX + H+ | - |
Mus musculus | mesoporphyrin IX + Fe2+ | - |
? | |
Fe2+-protoporphyrin + H+ | - |
Homo sapiens | protoporphyrin + Fe2+ | - |
? | |
Sn2+-protoporphyrin + H+ | - |
Homo sapiens | protoporphyrin + Sn2+ | - |
? | |
Zn2+-mesoporphyrin + H+ | - |
Mus musculus | mesoporphyrin + Zn2+ | - |
? | |
Zn2+-mesoporphyrin + H+ | - |
Homo sapiens | mesoporphyrin + Zn2+ | - |
? | |
Zn2+-protoporphyrin + H+ | - |
Mus musculus | protoporphyrin + Zn2+ | - |
? | |
Zn2+-protoporphyrin + H+ | - |
Homo sapiens | protoporphyrin + Zn2+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
heme synthase | - |
Mus musculus |
heme synthase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Mus musculus |