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Literature summary for 4.98.1.1 extracted from
- Porcine ferrochelatase: the relationship between iron-removal reaction and the conversion of heme to Zn-protoporphyrin (2010), Biosci. Biotechnol. Biochem., 74, 1415-1420.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Sus scrofa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
Zn2+ | pH 8.0, 37°C | Sus scrofa |  |
| 0.0057 | - |
hemin | pH 5.5, 45°C | Sus scrofa | |
| 0.0066 | - |
mesoporphyrin IX | pH 8.0, 37°C | Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | inner mitochondrial membrane | Sus scrofa | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, SDS-PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoheme + H+ | Sus scrofa | - |
protoporphyrin + Fe2+ | - |
r | |
| protoporphyrin + Fe2+ | Sus scrofa | the kcat of the forward reaction is about 11fold higher than the reverse reaction | protoheme + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA bead column chromatography, gel filtration | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | highest activity | Sus scrofa | - |
| LLC-PK1 cell | - |
Sus scrofa | - |
| muscle | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| heme + Zn2+ | - |
Sus scrofa | Zn-protoporphyrin + H+ | - |
? | |
| hemin + H+ | - |
Sus scrofa | mesoporphyrin IX + Zn2+ | - |
r | |
| mesoporphyrin IX + Zn2+ | - |
Sus scrofa | Zn2+-mesoporphyrin + 2 H+ | - |
r | |
| protoheme + H+ | - |
Sus scrofa | protoporphyrin + Fe2+ | - |
r | |
| protoporphyrin + Fe2+ | the kcat of the forward reaction is about 11fold higher than the reverse reaction | Sus scrofa | protoheme + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE | Sus scrofa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FeCH | - |
Sus scrofa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
hemin | pH 5.5, 45°C | Sus scrofa | |
| 6.7 | - |
Zn2+ | pH 8.0, 37°C | Sus scrofa | |
| 6.7 | - |
mesoporphyrin IX | pH 8.0, 37°C | Sus scrofa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | pH optimum for zinc removal | Sus scrofa |
| 7.5 | 8 | pH optimum for zinc insertion | Sus scrofa |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000092 | - |
hemin | pH 5.5, 45°C | Sus scrofa | |
| 0.00101 | - |
mesoporphyrin IX | pH 8.0, 37°C | Sus scrofa | |
| 0.00525 | - |
Zn2+ | pH 8.0, 37°C | Sus scrofa | |
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Release 2023.1 (January 2023)
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