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Literature summary for 4.98.1.1 extracted from

  • Leung, Y.K.; Wong, K.F.; Lee, H.K.; Ho, J.W.
    Cloning and characterization of chironomidae ferrochelatase: copper activation of the purified ferrochelatase (2004), Mol. Cell. Biochem., 262, 225-231.
    View publication on PubMed
Search for more literature for 4.98.1.1

Activating Compound

Activating Compound Comment Organism Structure
Cu2+ 0.1 mM, causes 4fold increase in activity Chironomus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Chironomus sp.

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
Mn2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
Pb2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
Zn2+ 0.1 mM, inhibits over 90% of the enzyme activity Chironomus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 0.1 mM, causes 4fold increase in activity Chironomus sp.
Fe2+ present in the cluster of the recombinant enzyme complex Chironomus sp.
Mg2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
Mn2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
Pb2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
Zn2+ 0.1 mM, inhibits over 90% of the enzyme activity Chironomus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41200
-
 monomer, calculated from amino acid sequence Chironomus sp.
43000
-
 x * 43000, SDS-PAGE Chironomus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrin + Fe2+ Chironomus sp.
-
 protoheme + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Chironomus sp. Q967G9 chironomid larvae
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chironomus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrin + Fe2+
-
 Chironomus sp. protoheme + H+
-
 ?
protoporphyrin IX + Fe2+ pH 8.0, 37°C Chironomus sp. protoheme + 2 H+
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Chironomus sp.

Synonyms

Synonyms Comment Organism
ferrochelatase
-
 Chironomus sp.