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4.98.1.1: protoporphyrin ferrochelatase

This is an abbreviated version!
For detailed information about protoporphyrin ferrochelatase, go to the full flat file.

Word Map on EC 4.98.1.1

Reaction

protoheme
+ 2 H+ =
protoporphyrin
 +
Fe2+

Synonyms

chelatase, ferro-, EC 4.99.1.1, FC1, FC2, FeC, FeCH, ferro-protoporphyrin chelatase, ferrochelatase, ferrochelatase 1, ferrochelatase I, ferrochelatase II, frataxin, HEM15, heme synthase, heme synthetase, hemH, HemH1, HemH2, hFC, host red cell ferrochelatase, iron chelatase, parasite genome-coded ferrochelatase, PfFC, PPIX ferrochelatase, protohaem ferrolyase, protoheme ferro-lyase, protoheme ferrolyase, protoheme lyase, protoporhyrin IX ferrochelatase, protoporphyrin (IX) ferrochelatase, protoporphyrin IX ferrochelatase, Shew_1140, Shew_2229, type II ferrochelatase

ECTree

     4 Lyases
         4.98 ATP-independent chelatases
             4.98.1 Forming coordination complexes
                4.98.1.1 protoporphyrin ferrochelatase

Cloned

Cloned on EC 4.98.1.1 - protoporphyrin ferrochelatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expression of just one native protein, ferrochelatase, in the presence of 60 microM delta-aminolevulinic acid (10 mg/l) is sufficient for 100% heme incorporation into three unrelated heme proteins derived from different organisms
-
Escherichia coli deltavis cells harbouring plasmids encoding active ferrochelatase mutants, wild-type murine ferrochelatase and mutants overexpressed, under the control of the Escherichia coli alkaline phosphatase promoter phoA, in Escherichia coli BL21(DE3) cells
-
Escherichia coli expressing His-tagged ferrochelatase
-
expressed in a Saccharomyces cerevisiae hem15-deficient strain
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli HB101 cells
expressed in Escherichia coli Rosetta 2 (DE3) cells
expressed in Oryza sativa cultivar Dongjin
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli JM109
expression in Escherichia coli, wild-type and mutants E289Q and E289A
ferrochelatase protein is induced in parallel with mitoferrin-1 and Abcb10 during Friend mouse erythroleukemia cell erythroid differentiation
-
into the eukaryotic expression plasmid pEYFP-N1, HeLa cells transiently transfected with YFP-tagged, wild-type FECH
-
into the expression vector pTrcHisA, expressed in Escherichia coli hemH deletion mutant
into the expression vector pTrcHisA, not expressed in Escherichia coli hemH deletion mutant
-
overexpression in Escherichia coli
recombinant full-length and deltaH324 mutant expressed in Escherichia coli using the pET9a-His6 vector
-
recombinant human ferrochelatase (R115L) expressed in Escherichia coli JM109 cells
wild-type and mutants expressed in Escherichia coli BL21(DE3)