Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C395delta | co-expressed with wild-type, 60% of wild-type activity (homogenate), 37.5-43% of wild-type activity (purified enzyme), exhibits a heterodimeric form when purified | Homo sapiens |
H157A | co-expressed with wild-type, 23-30% of wild-type activity (homogenate), loss of function (purified enzyme) | Homo sapiens |
H263A | co-expressed with wild-type, 23-30% of wild-type activity (homogenate), 37.5-43% of wild-type activity (purified enzyme), exhibits a heterodimeric form when purified | Homo sapiens |
H388A | co-expressed with wild-type, 23-30% of wild-type activity (homogenate), 37.5-43% of wild-type activity (purified enzyme), exhibits a heterodimeric form when purified | Homo sapiens |
I186T | causes erythropoietic protoporphyria, co-expressed with wild-type, no activity in the homogenate | Homo sapiens |
M267I | causes erythropoietic protoporphyria, co-expressed with wild-type, no activity in the homogenate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
mesoporphyrin | mutant H388A, 37°C | Homo sapiens | |
0.0085 | - |
mesoporphyrin | wild-type, 37°C | Homo sapiens | |
0.0092 | - |
mesoporphyrin | mutant H388A, co-expressed with wild-type, 37°C | Homo sapiens | |
0.0093 | - |
mesoporphyrin | mutant C395delta, co-expressed with wild-type, 37°C | Homo sapiens | |
0.0095 | - |
Zn2+ | mutant H263A, co-expressed with wild-type, 37°C | Homo sapiens | |
0.0105 | - |
Zn2+ | mutant H388A, co-expressed with wild-type, 37°C | Homo sapiens | |
0.0105 | - |
mesoporphyrin | mutant M267I, co-expressed with wild-type, 37°C | Homo sapiens | |
0.0108 | - |
Zn2+ | mutant H388A, 37°C | Homo sapiens | |
0.011 | - |
Zn2+ | mutant I186T, co-expressed with wild-type, 37°C | Homo sapiens | |
0.0111 | - |
mesoporphyrin | mutant I186T, co-expressed with wild-type, 37°C | Homo sapiens | |
0.0114 | - |
Zn2+ | wild-type, 37°C | Homo sapiens | |
0.012 | - |
mesoporphyrin | mutant H263A, 37°C | Homo sapiens | |
0.012 | - |
mesoporphyrin | mutant H263A, co-expressed with wild-type, 37°C | Homo sapiens | |
0.018 | - |
Zn2+ | mutant C395delta, co-expressed with wild-type, 37°C | Homo sapiens | |
0.024 | - |
Zn2+ | mutant M267I, co-expressed with wild-type, 37°C | Homo sapiens | |
0.04 | - |
Zn2+ | mutant H263A, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE, gel filtration, wild-type | Homo sapiens |
80000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrin + Fe2+ | Homo sapiens | - |
protoheme + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P22830 | - |
- |
Purification (Comment) | Organism |
---|---|
the recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mesoporphyrin + Zn2+ | 37°C | Homo sapiens | Zn-mesoporphyrin + 2 H+ | - |
? | |
protoporphyrin + Fe2+ | - |
Homo sapiens | protoheme + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE, gel filtration, wild-type | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ferrochelatase | - |
Homo sapiens |