Literature summary for 4.98.1.1 extracted from

Hansson, M.D.; Lindstam, M.; Hansson, M.
Crosstalk between metal ions in Bacillus subtilis ferrochelatase (2006), J. Biol. Inorg. Chem., 11, 325-333.

Search for more literature for 4.98.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
Mg2+ is required for the formation of Bacillus subtilis ferrochelatase crystals	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
E272S	more unstable than the wild-type, eliminates the stimulating effect of Mg2+	Bacillus subtilis
W230Y	more unstable than the wild-type, has 7% of the wild-type activity, does not display any Zn2+-induced quenching	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.024	-	Zn2+	wild-type, in the presence of 4 mM Mg2+, pH 7.4, room temperature	Bacillus subtilis
0.05	-	deuteroporphyrin IX	mutant E272S, in the presence of 4 mM Mg2+, pH 7.4, room temperature	Bacillus subtilis
0.054	-	deuteroporphyrin IX	mutant E272S, pH 7.4, room temperature	Bacillus subtilis
0.055	-	Zn2+	wild-type, pH 7.4, room temperature	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
inclusion body	the recombinant protein expressed in Escherichia coli	Bacillus subtilis	16234	-
soluble	in Bacillus subtilis	Bacillus subtilis	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	stimulates Zn2+ insertion by Bacillus subtilis ferrochelatase	Bacillus subtilis
Zn2+	quenches the fluorescence of Bacillus subtilis ferrochelatase	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
protoporphyrin + Fe2+	Bacillus subtilis	-	protoheme + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P32396	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deuteroporphyrin IX + Zn2+	-	Bacillus subtilis	zinc-deuteroporphyrin IX + H+	-	?
protoporphyrin + Fe2+	-	Bacillus subtilis	protoheme + H+	-	?
protoporphyrin IX + Zn2+	pH 7.4, room temperature	Bacillus subtilis	Zn protoporphyrin IX	-	?

Synonyms

Synonyms	Comment	Organism
ferrochelatase	-	Bacillus subtilis

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Release 2023.1 (January 2023)