Literature summary for 4.98.1.1 extracted from

Sobotka, R.; McLean, S.; Zuberova, M.; Hunter, C.N.; Tichy, M.
The C-terminal extension of ferrochelatase is critical for enzyme activity and for functioning of the tetrapyrrole pathway in Synechocystis PCC 6803 (2008), J. Bacteriol., 6, 2086-2095.

Search for more literature for 4.98.1.1

Application

Application	Comment	Organism
additional information	C-terminal extension is critical for activity of FeCH and it is strictly required for oligomerization of the enzyme	Synechocystis sp. PCC 6803

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant full-length and deltaH324 mutant expressed in Escherichia coli using the pET9a-His6 vector	Synechocystis sp. PCC 6803

Protein Variants

Protein Variants	Comment	Organism
additional information	deltaH324 mutant, mutated hemH gene, contains a truncated FeCH enzyme lacking the C-terminal domain, is localized to the membrane fraction, C-terminal domain is not necessary for membrane association of the enzyme, mutant grows poorly, has dramatically FeCH reduced activity, resulting in highly upregulated 5-aminolevulinic acid synthesis, suffers strongly from photooxidative stress as a consequence of disturbed tetrapyrrole biosynthesis and accumulation of phototoxic intermediates of this pathway. C1.8 mutant strain, carries a short deletion in Chl-binding CP47 protein, grows very slowly, complementation with the mutated hemH gene from mutant deltaH324	Synechocystis sp. PCC 6803

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Synechocystis sp. PCC 6803	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	deltaH324 mutant, immunoprecipitation	Synechocystis sp. PCC 6803
45000	-	deltaH324 mutant, gel filtration	Synechocystis sp. PCC 6803
46000	-	full-length enzyme, immunoprecipitation	Synechocystis sp. PCC 6803
60000	-	wild-type enzyme, gel filtration	Synechocystis sp. PCC 6803
160000	-	wild-type enzyme, gel filtration	Synechocystis sp. PCC 6803

Organism

Organism	UniProt	Comment	Textmining
Synechocystis sp. PCC 6803	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and deltaH324 mutant, by affinity chromatography and gel filtration	Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
protoporphyrin + Zn2+	-	Synechocystis sp. PCC 6803	Zn2+-protoporphyrin + H+	-	?

Subunits

Subunits	Comment	Organism
homodimer	wild-type enzyme, gel filtration	Synechocystis sp. PCC 6803
monomer	wild-type enzyme and deltaH324 mutant, gel filtration	Synechocystis sp. PCC 6803

Synonyms

Synonyms	Comment	Organism
FeCH	-	Synechocystis sp. PCC 6803

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Release 2023.1 (January 2023)