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Literature summary for 4.98.1.1 extracted from
- Biochemical characterization of protoporphyrinogen dehydrogenase and protoporphyrin ferrochelatase of Vibrio vulnificus and the critical complex formation between these enzymes (2018), Biochim. Biophys. Acta, 1862, 2674-2687 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Vibrio vulnificus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0038 | - |
Fe2+ | in complex with protoporphyrinogen dehydrogenase PgdH1, pH 8.0, 30°C | Vibrio vulnificus |  |
| 0.0039 | - |
Fe2+ | free enzyme, pH 8.0, 30°C | Vibrio vulnificus | |
| 0.009 | - |
protoporphyrin | in complex with protoporphyrinogen dehydrogenase PgdH1, pH 8.0, 30°C | Vibrio vulnificus | |
| 0.01 | - |
protoporphyrin | free enzyme, pH 8.0, 30°C | Vibrio vulnificus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | - |
Vibrio vulnificus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 315000 | - |
protoporphyrin ferrochelatase PpfC, gel filtration | Vibrio vulnificus |
| 512000 | - |
complex between protoporphyrinogen dehydrogenase PgdH1 and protoporphyrin ferrochelatase PpfC, gel filtration | Vibrio vulnificus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio vulnificus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| complex between protoporphyrinogen dehydrogenase PgdH1 and protoporphyrin ferrochelatase PpfC, recombinant protein | Vibrio vulnificus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrin + Fe2+ | - |
Vibrio vulnificus | protoheme + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | protoporphyrinogen dehydrogenase PgdH1 and protoporphyrin ferrochelatase PpfC form an 8:8 heterohexadecameric complex that is important for maintaining protoporphyrin IX at low levels. PpfC catalyzes Protoporphyrin IX efficiently whether or not it is part of the complex | Vibrio vulnificus |
| octamer | 8 * 38000, SDS-PAGE, free PpfC | Vibrio vulnificus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.51 | - |
protoporphyrin | in complex with protoporphyrinogen dehydrogenase PgdH1, pH 8.0, 30°C | Vibrio vulnificus | |
| 1.53 | - |
protoporphyrin | free enzyme, pH 8.0, 30°C | Vibrio vulnificus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2Fe-2S-center | PpfC contains a 2Fee2S cluster | Vibrio vulnificus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 150 | - |
protoporphyrin | free enzyme, pH 8.0, 30°C | Vibrio vulnificus | |
| 170 | - |
protoporphyrin | in complex with protoporphyrinogen dehydrogenase PgdH1, pH 8.0, 30°C | Vibrio vulnificus | |
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