6.3.2.17: tetrahydrofolate synthase
This is an abbreviated version!
For detailed information about tetrahydrofolate synthase, go to the full flat file.
Word Map on EC 6.3.2.17
-
6.3.2.17
-
methotrexate
-
antifolate
-
thymidylate
-
leukemia
-
polyglutamylation
-
folate-dependent
-
ccrf-cem
-
one-carbon
-
aminopterin
-
casei
-
glycinamide
-
mtx-resistant
-
methylenetetrahydrofolate
-
monoglutamate
-
ddathf
-
trimetrexate
-
transformylase
-
pemetrexed
-
raltitrexed
-
formyltransferase
-
leucovorin
-
7-hydroxymethotrexate
-
poly-gamma-glutamylation
-
5,10-methylenetetrahydrofolate
-
pentaglutamate
-
5-formyltetrahydrofolate
-
garft
-
5,10-dideazatetrahydrofolate
-
quinazoline
-
lometrexol
-
tomudex
-
folate-based
-
methotrexate-resistant
-
triglutamate
-
tetraglutamate
-
dihydropteroate
-
monoglutamylated
-
analysis
-
pteroylmonoglutamate
-
medicine
-
n10-propargyl-5,8-dideazafolic
-
pharmacology
- 6.3.2.17
- methotrexate
- antifolate
- thymidylate
- leukemia
-
polyglutamylation
-
folate-dependent
-
ccrf-cem
-
one-carbon
- aminopterin
- casei
- glycinamide
-
mtx-resistant
- methylenetetrahydrofolate
- monoglutamate
-
ddathf
- trimetrexate
-
transformylase
- pemetrexed
- raltitrexed
-
formyltransferase
- leucovorin
- 7-hydroxymethotrexate
-
poly-gamma-glutamylation
- 5,10-methylenetetrahydrofolate
- pentaglutamate
- 5-formyltetrahydrofolate
- garft
- 5,10-dideazatetrahydrofolate
- quinazoline
- lometrexol
- tomudex
-
folate-based
-
methotrexate-resistant
- triglutamate
- tetraglutamate
- dihydropteroate
-
monoglutamylated
- analysis
- pteroylmonoglutamate
- medicine
-
n10-propargyl-5,8-dideazafolic
- pharmacology
Reaction
Synonyms
100194131, AtDFB, cFPGS, DHFS/FPGS, dihydrofolate synthase/folylpolyglutamate synthase, eFPGS, Folate polyglutamate synthetase, FolC, folyl-gamma-glutamate synthetase, Folylpoly(.gamma.-glutamate) synthase, Folylpoly-.gamma.-glutamate synthase, Folylpoly-gamma-glutamate synthetase, Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase, Folylpolyglutamate synthase, Folylpolyglutamate synthetase, folylpolyglutamate synthetase 1, Folylpolyglutamyl synthetase, folypoly-gamma-glutamate synthetase, folypolyformyl glutamate synthase, folypolyglutamate synthase, folypolyglutamate synthetase, Formyltetrahydropteroyldiglutamate synthetase, FPGS, FPGS1, FPGS2, GRMZM2G393334, mFGPS, mFPGS, More, MTHFD1L, N10-Formyltetrahydropteroyldiglutamate synthetase, PfDHFS-FPGS, Synthetase, folylpolyglutamate, Tail length regulator, tetrahydrofolate:L-glutamate gamma-ligase (ADP-forming), tetrahydrofolylpolyglutamate synthase
ECTree
6.3.2.17 tetrahydrofolate synthaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 6.3.2.17 - tetrahydrofolate synthase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ADP + methotrexate-Glu3 + phosphate
ATP + methotrexate-Glu2 + L-glutamate
-
-
-
-
?
ADP + methotrexate-Glu4 + phosphate
ATP + methotrexate-Glu3 + L-glutamate
-
-
-
-
?
ATP + (2S)-2-(o-fluoro-p-(N-(2,7-dimethyl-4-oxo-3,4-dihydroquinazolin-6-yl)methyl)-N-(prop-2-ynyl-amino)benzamido)-4-(tetrazol-5-yl)butyric acid + L-glutamate
ADP + (2S)-2-(o-fluoro-p-(N-(2,7-dimethyl-4-oxo-3,4-dihydroquinazolin-6-yl)methyl)-N-(prop-2-ynyl-amino)benzamido)-4-(tetrazol-5-yl)butanoyl-Glu + phosphate
-
-
-
?
ATP + (2S)-2-(o-fluoro-p-(N-(2,7-dimethyl-4-oxo-3,4-dihydroquinazolin-6-yl)methyl)-N-(prop-2-ynyl-amino)benzamido)-4-(tetrazol-5-yl)butyric acid + L-glutamate
ADP + (2S)-2-(o-fluoro-p-(N-(2,7-dimethyl-4-oxo-3,4-dihydroquinazolin-6-yl)methyl)-N-(prop-2-ynyl-amino)benzamido)-4-(tetrazol-5-yl)butanoyl-L-Glu + phosphate
-
-
-
?
ATP + (6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6R)-10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + (6R)-5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + (6R)-5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
ATP in form of MgATP2-
-
-
?
ATP + (6R,S)-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-gamma-Glu
-
-
-
-
ir
ATP + (6RS)-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-Glu
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
ATP + (6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
poor substrate
-
?
ATP + (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5-methyl-5,6,7,8-tetrahydropteroyl-Glu2
-
poor substrate
-
?
ATP + (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5-methyl-5,6,7,8-tetrahydropteroyldiglutamate
-
-
-
-
?
ATP + (S)-2(5-(((1,2-dihydro-3-methyl-1-oxobenzo-(f)quinazolin-9-yl)methyl)-1-oxo-2-isoindolinyl))glutaric acid + L-glutamate
ADP + (S)-2(5-(((1,2-dihydro-3-methyl-1-oxobenzo-(f)quinazolin-9-yl)methyl)-1-oxo-2-isoindolinyl))glutaryl-L-Glu + phosphate
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + 3-(N-phosphonoacetyl)amino-2-L-(N-pteroylamino)propanoic acid + L-glutamate
ADP + 3-(N-phosphonoacetyl)amino-2-L-(N-pteroylamino)propanoyl-Glu + phosphate
-
much less effective than folic acid
-
?
ATP + 3-N-(methoxyphosphono)acetylamino-2-L-(N-pteroylamino)propanoic acid + L-glutamate
ADP + 3-N-(methoxyphosphono)acetylamino-2-L-(N-pteroylamino)propanoyl-Glu + phosphate
-
much less effective than folic acid
-
?
ATP + 5,10-dideaza-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + 5,10-dideaza-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
ATP + 5,10-dideazatetrahydrofolic acid + L-glutamate
ADP + 5,10-dideazatetrahydrofolyl-L-Glu + phosphate
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu3
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-L-Glu
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2 + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)3
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
ATP + 5,10-methylene-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,10-methylene-tetrahydrofolyl-Glu3
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolate + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
-
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu3
ATP + 5,6,7,8-tetrahydrofolic acid + L-Glu
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
ATP + 5,6,7,8-tetrahydrofolyl-beta-L-Ala-L-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-beta-L-Ala-L-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolyl-gamma-(L-Glu)2 + L-Glu
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-gamma-(L-Glu)3
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glun
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu + L-Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-gamma-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2
ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu4
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu4
ATP + 5,6,7,8-tetrahydropteroyl-Glu4 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu5
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu4 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu5
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu6
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu6
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
ATP + 5,6,7,8-tetrahydropteroyl-Gly-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Gly-Glu2
ATP + 5-formyl-5,6,7,8-tetrahydrofolate + L-glutamate
ADP + 5-formyl-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
ATP + 5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-Glu2
ATP + 5-methyl-H4-homofolate + L-glutamate
ADP + phosphate + 5-methyl-H4-homofolyl-Glu
-
-
-
-
?
ATP + 6-R 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu3
-
at similar rate as that seen with aminopterin as substrate, endogenous enzyme isolated from liver. More efficient than aminopterin, recombinant liver enzyme
-
?
ATP + 6-S 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu3
-
more efficient than aminopterin, recombinant liver enzyme
-
?
ATP + 7,8-dihydropteroate + L-Glu
ADP + phosphate + 7,8-dihydropteroyl-L-Glu
-
-
-
?
ATP + 7,8-dihydropteroyl-(4-aminobutanoyl)-Glu + Glu
ADP + phosphate + 7,8-dihydropteroyl-(4-aminobutanoyl)-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroyl-(4-aminobutanoyl)-Glu + L-glutamate
ADP + phosphate + 7,8-dihydropteroyl-(4-aminobutanoyl)-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroyl-(5-aminovaleryl)-Glu + Glu
ADP + phosphate + 7,8-dihydropteroyl-(5-aminovaleryl)-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroyl-4-aminobutanoyl-Glu + Glu
ADP + phosphate + 7,8-dihydropteroyl-4-aminobutanoyl-Glun
-
-
n: 3-5
?
ATP + 7,8-dihydropteroyl-beta-Ala-Glu + Glu
ADP + phosphate + 7,8-dihydropteroyl-beta-Ala-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroyl-Glu + L-glutamate
ADP + phosphate + 7,8-dihydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroyl-Glu + L-glutamate
ADP + phosphate + 7,8-dihydropteroyl-Glu2
-
-
-
-
?
ATP + 7-hydroxymethotrexate + Glu
ADP + phosphate + 7-hydroxymethotrexyl-Glu
-
-
-
-
?
ATP + 9-methylpteroyl-L-glutamic acid + Glu
ADP + phosphate + 9-methylpteroyldiglutamate
-
-
-
?
ATP + dichloromethotrexate + Glu
ADP + phosphate + dichloromethotrexyl-Glu
-
-
-
-
?
ATP + dihydropteroic acid + L-glutamate
ADP + dihydropteroyl-Glu
-
enhancement of ATP binding is observed in the presence of this substrate. The substrate causes a conformational change in the inactive fraction of the enzyme which allows it to bind ATP
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydrofolate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydrofolate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + tetrahydrofolate-Glu
ADP + phosphate + tetrahydrofolyl-Glu2
-
assay at pH 10, 37°C
-
-
?
ATP + L-homocysteate + Glu
ADP + phosphate + (6RS)-5-methyl-H4-homofolyl-Glu
-
-
-
-
?
ATP + methotrexate + 3,3-difluoroglutamate
ADP + phosphate + methotrexyl-3,3-difluoroglutamate
ATP + methotrexate + L-glutamate
ADP + phosphate + L-glutamyl-methotrexate
-
-
-
-
?
ATP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-glutamic acid + L-glutamate
ADP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-Glu2 + phosphate
ATP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-glutamic acid + L-glutamate
ADP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-Glu2 + phosphate
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate
ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)-Glu2
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate
ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)Glu2
-
folic acid, poor substrate
-
?
ATP + Nalpha-(4-amino-4-deoxy-5,8-diazapteroyl)-L-ornithine + L-glutamate
?
-
-
-
?
ATP + Nalpha-(4-amino-4-deoxy-5,chloropteroyl)-L-ornithine + L-glutamate
?
-
-
-
?
ATP + Nalpha-(4-amino-4-deoxy-8-deazapteroyl)-L-ornithine + L-glutamate
?
-
-
-
?
ATP + Nalpha-(4-amino-4-deoxy-N10-methylpteroyl)-Nepsilon-(phosphonoacetyl)-L-lysine + L-glutamate
?
-
much less effective than folic acid
-
?
ATP + Nalpha-(4-amino-4-deoxypteroyl)-Ndelta-hemiphthaloyl-L-ornithine + L-glutamate
?
-
-
-
?
ATP + Nalpha-(5,8-dideaza-5-chloropteroyl)-L-ornithine + L-glutamate
?
-
-
-
?
ATP + pteroyl-(4-aminobutanoyl)-Glu + Glu
ADP + phosphate + pteroyl-(4-aminobutanoyl)-Glu2
-
-
-
-
?
ATP + pteroyl-(5-aminopentanoyl)-Glu + Glu
ADP + phosphate + pteroyl-(5-aminopentanoyl)-Glu2
-
-
-
-
?
ATP + tetrahydroaminopterin + Glu
ADP + phosphate + tetrahydroaminopteryl-Glu
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
CTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
CDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
dATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + Glu
dADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
?
dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
dATP + aminopterin + Glu
dADP + phosphate + aminopteryl-Glu
-
112% of the activity relative to ATP
-
-
?
dGTP + aminopterin + Glu
dGDP + phosphate + aminopteryl-Glu
-
37% of the activity relative to ATP
-
-
?
GTP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu + Glu
GDP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
?
ITP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + Glu
IDP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
?
ITP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
IDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
UTP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + Glu
UDP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
?
UTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
UDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
ADP + phosphate + 7,8-dihydropteroyl-Glu
ATP + 7,8-dihydropteroate + L-glutamate
-
-
-
-
?
ADP + phosphate + 7,8-dihydropteroyl-Glu
ATP + 7,8-dihydropteroate + L-glutamate
-
-
-
-
?
ATP + (6RS)-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-Glu
-
not as effective as 5,10-methylene-5,6,7,8-tetrahydrofolic acid
-
?
ATP + (6RS)-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-Glu
-
-
-
-
ir
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 3,5-difluoromethotrexate + Glu
ADP + phosphate + 3,5-difluoromethotrexyl-Glu
-
-
-
?
ATP + 3,5-difluoromethotrexate + Glu
ADP + phosphate + 3,5-difluoromethotrexyl-Glu
-
-
-
?
ATP + 3,5-difluoropteroyl-Glu + Glu
ADP + phosphate + 3,5-difluoropteroyl-Glu2
-
-
-
?
ATP + 3,5-difluoropteroyl-Glu + Glu
ADP + phosphate + 3,5-difluoropteroyl-Glu2
-
-
-
?
ATP + 5,10-dideaza-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + 5,10-dideaza-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
-
lometrexol
-
?
ATP + 5,10-dideaza-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + 5,10-dideaza-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
-
-
-
?
ATP + 5,10-dideaza-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + 5,10-dideaza-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
-
lometrexol
-
?
ATP + 5,10-dideazatetrahydrofolic acid + L-glutamate
ADP + 5,10-dideazatetrahydrofolyl-L-Glu + phosphate
-
-
-
?
ATP + 5,10-dideazatetrahydrofolic acid + L-glutamate
ADP + 5,10-dideazatetrahydrofolyl-L-Glu + phosphate
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 3
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 2
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 3
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 2
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 5
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 7
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 6
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu3
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu3
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
-
-
?
ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
-
-
?
ATP + 5,6,7,8-tetrahydropteroate + Glu
ADP + phosphate + 5,6,7,8-terahydropteroyl-Glu
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroate + Glu
ADP + phosphate + 5,6,7,8-terahydropteroyl-Glu
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glun
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glun
-
-
n: 3-5
?
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
ATP in form of MgATP2-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
ATP in form of MgATP2-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-gamma-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
gaba i.e. gamma-aminobutanoyl
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-gamma-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
gaba i.e. gamma-aminobutanoyl
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu4
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu4
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu6
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu6
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 3
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 2
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 3
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 5
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 2
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 4
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 7
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 6
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Gly-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Gly-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Gly-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Gly-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 2
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroate + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glu
-
-
-
-
?
ATP + 7,8-dihydropteroate + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glu
-
-
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
-
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 5
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 5
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 2
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 2
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
most active substrate
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
products with a glutamate chain length of 2-4 are formed
?
ATP + aminopterin + L-glutamate
?
-
more efficient than 6-S 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2 and 6-R 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2, recombinant enzyme from leukemic cells
-
?
ATP + dihydroaminopterin + Glu
ADP + phosphate + dihydroaminopteryl-Glu
-
-
-
?
ATP + methotrexate + 3,3-difluoroglutamate
ADP + phosphate + methotrexyl-3,3-difluoroglutamate
-
-
-
?
ATP + methotrexate + 3,3-difluoroglutamate
ADP + phosphate + methotrexyl-3,3-difluoroglutamate
-
-
-
-
?
ATP + methotrexate + Glu
ADP + phosphate + methotrexyl-Glu
-
-
-
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
-
-
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
-
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
-
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
-
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
CHO cells expressing the human enzyme accumulate more metabolized drug than CHO cells expressing equivalent levels of endogenous hamster enzyme. Cells expressing only the mitochondrial isozyme do not metabolize this substrate
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
T-ALL and AML patients show inefficient polyglutamylation due to lower enzyme activity
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
-
-
?
ATP + methotrexate-Glun + Glu
ADP + phosphate + methotrexyl-Glun+1
-
n: 3
-
?
ATP + methotrexate-Glun + Glu
ADP + phosphate + methotrexyl-Glun+1
-
n: 5
-
?
ATP + methotrexate-Glun + Glu
ADP + phosphate + methotrexyl-Glun+1
-
n: 2
-
?
ATP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-glutamic acid + L-glutamate
ADP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-Glu2 + phosphate
-
-
-
?
ATP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-glutamic acid + L-glutamate
ADP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-Glu2 + phosphate
-
pemetrexed
-
?
ATP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-glutamic acid + L-glutamate
ADP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-Glu2 + phosphate
-
-
-
?
ATP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-glutamic acid + L-glutamate
ADP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-Glu2 + phosphate
-
raltitrexed
-
?
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate
ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)-Glu2
-
folic acid
-
?
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate
ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)-Glu2
-
folic acid
-
?
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate
ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)-Glu2
-
folic acid
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 2
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 2
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
-
?
ATP + tetrahydrofolate-Glu + L-glutamate
ADP + phosphate + tetrahydrofolyl-Glu2
-
-
-
?
ATP + tetrahydrofolate-Glu + L-glutamate
ADP + phosphate + tetrahydrofolyl-Glu2
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
CTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
CDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
CTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
CDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
at 13% of the activity relative to ATP
-
-
?
dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
as effective as ATP
-
-
?
dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
UTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
UDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
UTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
UDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
additional information
?
-
-
uses 5,10-methylenen-5,6,7,8-tetrahydropteroylpolyglutamate as the preferred, and sometimes only polyglutamate substrate
-
-
?
additional information
?
-
-
enzyme in biosynthesis of folylpolyglutamates , the most universal functions of folylpolyglutamates: 1. Actual cofactors in vivo for folate dependent enzymes, 2. Inhibitor of folate dependent enzymes for which they are not substrates, 3. Increases retention of folates after they are transported into cells as monoglutamates
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
pteroate and pteroylmonoglutamate substrates are utilized much more effectively than the polyglutamate derivatives. The most effective substrates are: 5,6,7,8-tetrahydropteroate, tetrahydrofolate, 5,10-methylene-tetrahydrofolate. The most effective diglutamate substrate is : 5,10-methylene-5,6,7,8-tetrahydropteroyldiglutamate
-
-
?
additional information
?
-
-
monoglutamyl form and polyglutamyl forms of 5,6,7,8-tetrahydropteroate are the preferred substrates
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
the enzyme catalyzes the MgATP-dependent ligation of L-glutamate to tetrahydrofolate coenzymes or to antifolates at the gamma-carboxyl of the terminal glutamate to form polyglutamate derivatives
-
?
additional information
?
-
-
enzyme in biosynthesis of folylpolyglutamates , the most universal functions of folylpolyglutamates: 1. Actual cofactors in vivo for folate dependent enzymes, 2. Inhibitor of folate dependent enzymes for which they are not substrates, 3. Increases retention of folates after they are transported into cells as monoglutamates
-
-
?
additional information
?
-
-
mitochondrial enzyme is required for mitochondrial one carbon metabolism and for normal one carbon flux in the cytosol
-
-
?
additional information
?
-
-
depressed activity of the enzyme is the predominant mechanism of high level resistance to polyglutamylation-dependent antifolates
-
?
additional information
?
-
-
mitochondrial enzyme activity is required for mitochondrial folate accumulation, and cells lacking this isozyme are auxotrophic for glycine. Overexpression of cytosolic enzyme does not complement the lack of mitochondrial activity
-
?
additional information
?
-
-
enzyme catalyzes the addition of glutamate residues to folates and antifolates to form the physiological active coenzymatic forms of the vitamin and more potent anti-folate agents
-
-
?
additional information
?
-
-
central enzyme in establishing and maintaining folylpolyglutamate pools in whole cells
-
?
additional information
?
-
-
the enzyme catalyzes the formation of an amide bond between glutamic acid and the gamma-carboxyl group of any of the naturally occurring folate compounds
-
?
additional information
?
-
-
the enzyme catalyzes the MgATP-dependent addition of glutamate residues to the gamma-carboxyl of folates to generate folylpolyglutamates
-
?
additional information
?
-
-
the enzyme synthesizes folate and antifolate poly(gamma-glutamate) metabolites
-
?
additional information
?
-
-
folylpoly-gamma-glutamate synthetase can catalyze the processive addition of approximately four glutamate residues to (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroylglutamic acid. The degree of processivity is dependent on the concentration of the folate substrate, thus suggesting a mechanism for the regulation of folate polyglutamate synthesis in cells
-
-
?
additional information
?
-
-
no dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
no dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
no dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
the enzyme catalyzes the MgATP-dependent ligation of L-glutamate to tetrahydrofolate coenzymes or to antifolates at the gamma-carboxyl of the terminal glutamate to form polyglutamate derivatives
-
?
additional information
?
-
-
the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell
-
?
additional information
?
-
there are two possible sites for binding folate substrates. The di- and triglutamate substrates can be accommodated in both sites in a way appropriate for the ligation reaction, but only the new pocket appears to bind mTHF and allow the initial ligation reaction. It is suggested that the new pocket plays an essential role in the first turnover to create folylpoly-glutamates
-
-
?
additional information
?
-
-
there are two possible sites for binding folate substrates. The di- and triglutamate substrates can be accommodated in both sites in a way appropriate for the ligation reaction, but only the new pocket appears to bind mTHF and allow the initial ligation reaction. It is suggested that the new pocket plays an essential role in the first turnover to create folylpoly-glutamates
-
-
?
additional information
?
-
-
most effective substrates are: 7,8-dihydrofolate, 5,6,7,8-tetrahydrofolate, 5,10-methylenetetrahydrofolate, 10-formyltetrahydrofolate, 5-formyltetrahydrofolate
-
-
?
additional information
?
-
-
alterations in the amino terminal structure of the enzyme may influence its catalytic and/or substrate binding properties
-
?
additional information
?
-
-
depressed activity of the enzyme is the predominant mechanism of high level resistance to polyglutamylation-dependent antifolates
-
?
additional information
?
-
-
the enzyme catalyzes the consecutive addition of one or more L-glutamyl moieties to the gamma-carboxyl group in natural folates and glutamate-containing antifolates. Cells deficient in this enzyme are auxothrophic for thymidine and a purine
-
?
additional information
?
-
-
the enzyme catalyzes the formation of polyglutamates from folates and folate antimetabolites in mammalian cells
-
?
additional information
?
-
-
the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell
-
?
additional information
?
-
-
folylpolyglutamate synthesis is not mediated by a single enzyme, but by at least two soluble enzymes which are products of the mac and met-6 loci respectively
-
-
?
additional information
?
-
-
the expression of the enzyme in Escherichia coli and Saccharomyces cerevisiae defective strains restores Escherichia coli cells to methionine and glycine prototrophy on minimal medium, but cannot entirely compensate for the loss of the native homologue in Saccharomyces cerevisiae. The malarial gene encodes a protein carrying dihydrofolate synthetase and folylpolyglutamate synthetase activities, both needed for the de novo folate synthesis
-
?
additional information
?
-
-
no dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
met7 mutant strain is a methionine auxotroph and an adenine and thymidine auxotroph when grown in the presence of sulfanilamide. Met7 mutants have a petite phenotype, defective in mitochondrial respiration and unable to grow on glycerol or other nonfermentable substrates as a carbon/energy source. Disruption of met7 causes deficiencies in folate metabolism and loss of both cytoplasmic and mitochondrial enzyme activity. Met7 mutants are complemented by genes that express only cytoplasmic enzyme
-
?
additional information
?
-
-
enzyme catalyzes the addition of glutamate residues to folates and antifolates to form the physiological active coenzymatic forms of the vitamin and more potent anti-folate agents
-
-
?
additional information
?
-
-
the enzyme catalyzes the consecutive addition of one or more L-glutamyl moieties to the gamma-carboxyl group in natural folates and glutamate-containing antifolates. Cells deficient in this enzyme are auxothrophic for thymidine and a purine
-
?
