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Literature summary for 6.3.2.17 extracted from

  • Clarke, L.; Waxman, D.J.
    Human liver folylpolyglutamate synthetase: biochemical characterization and interactions with folates and folate antagonists (1987), Arch. Biochem. Biophys., 256, 585-596.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 45 mM: 3.5fold enhancement of activity, 200 mM: 25% decrease in activity Homo sapiens

General Stability

General Stability Organism
2-mercaptoethanol enhances stabilization provided by ATP. ATP stabilizes against heat inactivation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0043
-
aminopterin
-
Homo sapiens
0.011
-
methotrexate-Glu4 methotrexate-Glu5 Homo sapiens
0.02
-
methotrexate-Glu3
-
Homo sapiens
0.029
-
methotrexate-Glu2
-
Homo sapiens
0.054
-
methotrexate
-
Homo sapiens
0.25
-
ATP reaction with aminopterin + ATP Homo sapiens
1.2
-
L-Glu reaction with aminopterin + ATP Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required form maximal activity Homo sapiens
K+ Km: 1.6 mM Homo sapiens
Mg2+ required Homo sapiens
Mg2+ Km: 3.7 mM Homo sapiens
NaHCO3 activates Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + methotrexate-Glu3 + phosphate
-
Homo sapiens ATP + methotrexate-Glu2 + L-glutamate
-
?
ADP + methotrexate-Glu4 + phosphate
-
Homo sapiens ATP + methotrexate-Glu3 + L-glutamate
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
-
Homo sapiens ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
ATP + aminopterin + Glu
-
Homo sapiens ADP + phosphate + aminopteryl-Glu
-
?
ATP + methotrexate + Glu
-
Homo sapiens ADP + phosphate + methotrexyl-Glu
-
?
ATP + methotrexate-Glu + L-glutamate
-
Homo sapiens ADP + methotrexate-Glu2 + phosphate
-
?
ATP + methotrexate-Glun + Glu n: 3 Homo sapiens ADP + phosphate + methotrexyl-Glun+1
-
?
ATP + methotrexate-Glun + Glu n: 5 Homo sapiens ADP + phosphate + methotrexyl-Glun+1
-
?
ATP + methotrexate-Glun + Glu n:4 Homo sapiens ADP + phosphate + methotrexyl-Glun+1
-
?
ATP + methotrexate-Glun + Glu n: 2 Homo sapiens ADP + phosphate + methotrexyl-Glun+1
-
?
ATP + pteroyl-Glu + Glu n: 1, i.e. pteroylmonoglutamate, folic acid Homo sapiens ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
dATP + aminopterin + Glu 112% of the activity relative to ATP Homo sapiens dADP + phosphate + aminopteryl-Glu
-
?
dGTP + aminopterin + Glu 37% of the activity relative to ATP Homo sapiens dGDP + phosphate + aminopteryl-Glu
-
?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
40 negligible activity at 0°C and above 40°C Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
t1/2: 8 min, in absence of ATP, t1/2: 38 min, stabilized by 5 mM ATP Homo sapiens
37
-
t1/2: 68 min, in absence of ATP Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.4
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 about 50% of maximal activity at pH 7.5 and 9 Homo sapiens