Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 45 mM: 3.5fold enhancement of activity, 200 mM: 25% decrease in activity | Homo sapiens |
General Stability | Organism |
---|---|
2-mercaptoethanol enhances stabilization provided by ATP. ATP stabilizes against heat inactivation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
aminopterin | - |
Homo sapiens | |
0.011 | - |
methotrexate-Glu4 | methotrexate-Glu5 | Homo sapiens | |
0.02 | - |
methotrexate-Glu3 | - |
Homo sapiens | |
0.029 | - |
methotrexate-Glu2 | - |
Homo sapiens | |
0.054 | - |
methotrexate | - |
Homo sapiens | |
0.25 | - |
ATP | reaction with aminopterin + ATP | Homo sapiens | |
1.2 | - |
L-Glu | reaction with aminopterin + ATP | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required form maximal activity | Homo sapiens | |
K+ | Km: 1.6 mM | Homo sapiens | |
Mg2+ | required | Homo sapiens | |
Mg2+ | Km: 3.7 mM | Homo sapiens | |
NaHCO3 | activates | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + methotrexate-Glu3 + phosphate | - |
Homo sapiens | ATP + methotrexate-Glu2 + L-glutamate | - |
? | |
ADP + methotrexate-Glu4 + phosphate | - |
Homo sapiens | ATP + methotrexate-Glu3 + L-glutamate | - |
? | |
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu | - |
Homo sapiens | ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1 | - |
? | |
ATP + aminopterin + Glu | - |
Homo sapiens | ADP + phosphate + aminopteryl-Glu | - |
? | |
ATP + methotrexate + Glu | - |
Homo sapiens | ADP + phosphate + methotrexyl-Glu | - |
? | |
ATP + methotrexate-Glu + L-glutamate | - |
Homo sapiens | ADP + methotrexate-Glu2 + phosphate | - |
? | |
ATP + methotrexate-Glun + Glu | n: 3 | Homo sapiens | ADP + phosphate + methotrexyl-Glun+1 | - |
? | |
ATP + methotrexate-Glun + Glu | n: 5 | Homo sapiens | ADP + phosphate + methotrexyl-Glun+1 | - |
? | |
ATP + methotrexate-Glun + Glu | n:4 | Homo sapiens | ADP + phosphate + methotrexyl-Glun+1 | - |
? | |
ATP + methotrexate-Glun + Glu | n: 2 | Homo sapiens | ADP + phosphate + methotrexyl-Glun+1 | - |
? | |
ATP + pteroyl-Glu + Glu | n: 1, i.e. pteroylmonoglutamate, folic acid | Homo sapiens | ADP + phosphate + pteroylmonoglutamyl-Glu | - |
? | |
dATP + aminopterin + Glu | 112% of the activity relative to ATP | Homo sapiens | dADP + phosphate + aminopteryl-Glu | - |
? | |
dGTP + aminopterin + Glu | 37% of the activity relative to ATP | Homo sapiens | dGDP + phosphate + aminopteryl-Glu | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
40 | negligible activity at 0°C and above 40°C | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
t1/2: 8 min, in absence of ATP, t1/2: 38 min, stabilized by 5 mM ATP | Homo sapiens |
37 | - |
t1/2: 68 min, in absence of ATP | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.4 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | about 50% of maximal activity at pH 7.5 and 9 | Homo sapiens |