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6.3.2.17: tetrahydrofolate synthase

This is an abbreviated version!
For detailed information about tetrahydrofolate synthase, go to the full flat file.

Word Map on EC 6.3.2.17

Reaction

ATP
+
tetrahydropteroyl-[gamma-Glu]n
 +
L-glutamate
=
ADP
 +
phosphate
 +
tetrahydropteroyl-[gamma-Glu]n+1

Synonyms

100194131, AtDFB, cFPGS, DHFS/FPGS, dihydrofolate synthase/folylpolyglutamate synthase, eFPGS, Folate polyglutamate synthetase, FolC, folyl-gamma-glutamate synthetase, Folylpoly(.gamma.-glutamate) synthase, Folylpoly-.gamma.-glutamate synthase, Folylpoly-gamma-glutamate synthetase, Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase, Folylpolyglutamate synthase, Folylpolyglutamate synthetase, folylpolyglutamate synthetase 1, Folylpolyglutamyl synthetase, folypoly-gamma-glutamate synthetase, folypolyformyl glutamate synthase, folypolyglutamate synthase, folypolyglutamate synthetase, Formyltetrahydropteroyldiglutamate synthetase, FPGS, FPGS1, FPGS2, GRMZM2G393334, mFGPS, mFPGS, More, MTHFD1L, N10-Formyltetrahydropteroyldiglutamate synthetase, PfDHFS-FPGS, Synthetase, folylpolyglutamate, Tail length regulator, tetrahydrofolate:L-glutamate gamma-ligase (ADP-forming), tetrahydrofolylpolyglutamate synthase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.17 tetrahydrofolate synthase

KM Value

KM Value on EC 6.3.2.17 - tetrahydrofolate synthase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002 - 0.0029
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate
0.00193
(6R)-5,10-dideaza-5,6,7,8-tetrahydrofolate
-
-
0.00087
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
-
-
0.00096
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
0.0016
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
0.00105
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
0.0145
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
1
(6RS)-5,6,7,8-tetrahydrofolic acid
-
pH 9.75
0.00106
(6S)-5,10-dideaza-5,6,7,8-tetrahydrofolate
-
-
0.00116
(6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
-
-
0.0014 - 0.0063
(6S)-5,6,7,8-tetrahydropteroylglutamate
0.075 - 0.122
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate
0.054 - 0.064
(6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate
0.0022 - 0.014
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
0.41
3,3-difluoroglutamate
-
reaction with methotrexate + ATP
0.0031 - 0.0054
3,3-difluoromethotrexate
0.0012 - 0.0076
5,10-dideazatetrahydrofolic acid
0.03 - 3.8
5,10-methylene-5,6,7,8-tetrahydrofolic acid
0.01 - 0.204
5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2
0.0048 - 0.057
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
0.0023
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
-
0.00096
5,6,7,8-tetrahydrofolate
-
-
0.00123
5,6,7,8-tetrahydrofolate-Glu2
-
pH 8.7
0.01 - 0.655
5,6,7,8-tetrahydrofolic acid
0.16
5,6,7,8-tetrahydrofolyl-beta-L-Ala-L-Glu
-
L-Glu
-
0.3
5,6,7,8-tetrahydrofolyl-gamma-(L-Glu)2
mutant D154A, pH 9.8
0.074
5,6,7,8-tetrahydropteroyl-(4-aminobutanoyl)-Glu
-
-
0.16
5,6,7,8-tetrahydropteroyl-beta-Ala-Glu
-
L-Glu
0.018 - 0.16
5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu
-
0.0021 - 6.8
5,6,7,8-tetrahydropteroyl-Glu
0.0033 - 0.0034
5,6,7,8-tetrahydropteroyl-Glu2
0.0011 - 0.0014
5,6,7,8-tetrahydropteroyl-Glu3
0.0016 - 0.002
5,6,7,8-tetrahydropteroyl-Glu4
0.0027
5,6,7,8-tetrahydropteroyl-Glu5
0.0081 - 0.105
5-formyl-5,6,7,8-tetrahydropteroyl-Glu
0.013
5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
0.444
5-methyl-H4-homofolate
-
-
0.0063 - 0.98
7,8-dihydropteroate
0.012
7,8-dihydropteroyl-(4-aminobutanoyl)-Glu
0.022
7,8-dihydropteroyl-(5-aminopentanoyl)-Glu
-
-
0.076
7,8-dihydropteroyl-beta-Ala-Glu
-
-
0.00081 - 0.0086
7,8-dihydropteroyl-Glu
0.0026 - 0.048
7,8-dihydropteroyl-Glu2
0.004 - 0.18
aminopterin
0.0046 - 35.7
ATP
0.05
dATP
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.0073
dihydroaminopterin
-
-
0.332 - 5.984
Glu
0.235
glutamic acid
-
pH 8.9, 37ºC, charcoal assay, wild type recombinant enzyme
0.136
ITP
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.053 - 150
L-Glu
0.24 - 26.69
L-glutamate
0.43 - 350
L-Glutamic acid
0.019 - 0.138
methotrexate
0.063 - 0.074
methotrexate-Glu
0.029
methotrexate-Glu2
-
-
0.02
methotrexate-Glu3
0.011
methotrexate-Glu4
-
methotrexate-Glu5
0.018
MgATP2-
-
ATP
0.126 - 0.14
N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid
0.81
pteroyl-(4-aminobutanoyl)-Glu
-
-
1.09
pteroyl-(5-aminopentanoyl)-Glu
-
-
0.69
pteroyl-beta-Ala-Glu
-
-
0.059 - 0.137
pteroyl-Glu
0.016
pteroyl-Glu2
-
-
0.119
pteroyl-Glu3
-
-
0.064
pteroyl-Glu5
-
-
0.962
pteroylglutamate
-
-
0.093
pteroylmonoglutamate
-
-
0.062
pteroylmonoglutamyl-Glu
0.19
UTP
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP