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6.3.2.17: tetrahydrofolate synthase

This is an abbreviated version!
For detailed information about tetrahydrofolate synthase, go to the full flat file.

Word Map on EC 6.3.2.17

Reaction

ATP
+
tetrahydropteroyl-[gamma-Glu]n
 +
L-glutamate
=
ADP
 +
phosphate
 +
tetrahydropteroyl-[gamma-Glu]n+1

Synonyms

100194131, AtDFB, cFPGS, DHFS/FPGS, dihydrofolate synthase/folylpolyglutamate synthase, eFPGS, Folate polyglutamate synthetase, FolC, folyl-gamma-glutamate synthetase, Folylpoly(.gamma.-glutamate) synthase, Folylpoly-.gamma.-glutamate synthase, Folylpoly-gamma-glutamate synthetase, Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase, Folylpolyglutamate synthase, Folylpolyglutamate synthetase, folylpolyglutamate synthetase 1, Folylpolyglutamyl synthetase, folypoly-gamma-glutamate synthetase, folypolyformyl glutamate synthase, folypolyglutamate synthase, folypolyglutamate synthetase, Formyltetrahydropteroyldiglutamate synthetase, FPGS, FPGS1, FPGS2, GRMZM2G393334, mFGPS, mFPGS, More, MTHFD1L, N10-Formyltetrahydropteroyldiglutamate synthetase, PfDHFS-FPGS, Synthetase, folylpolyglutamate, Tail length regulator, tetrahydrofolate:L-glutamate gamma-ligase (ADP-forming), tetrahydrofolylpolyglutamate synthase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.17 tetrahydrofolate synthase

Crystallization

Crystallization on EC 6.3.2.17 - tetrahydrofolate synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop method in 1.2-1.7 M ammonium sulfate
-
enzyme-MgAMPPCP complex is produced by soaking apoFPGS crystals, grown overnight in a solution containing 20% PEG4000, 10 mM AMPPCP, 20 mM MgCl2, 100 mM KCl and 50 mM CAPSO, pH 9.0. The enzyme has a ATP-binding pocket in deep cleft formed between the N and C-domains, the folate-binding site is located in the interdomain cleft, His316, Ser412 and Lys346 are involved in the L-glutamate binding site
hanging drop vapour diffusion method using 50 mM acetate buffer pH 5.3 and 6% PEG 4000
microseeding, E143A mutant
-
batch method in the presence ADP and CoCl2 or vapour diffusion in the presence of ADP, dihydrofolate and CaCl2
-
in complex with ADP and with adenylyl 5'-(beta,gamma-ethylene)diphosphonate at 2.0 and 2.3 A resolution, respectively. The nucleotide-binding site is deeply buried, while the binding of folate substrates occurs in a long extended groove. The active site beta5-alpha6-loop adopts an open position