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6.3.2.17: tetrahydrofolate synthase

This is an abbreviated version!
For detailed information about tetrahydrofolate synthase, go to the full flat file.

Word Map on EC 6.3.2.17

Reaction

ATP
+
tetrahydropteroyl-[gamma-Glu]n
 +
L-glutamate
=
ADP
 +
phosphate
 +
tetrahydropteroyl-[gamma-Glu]n+1

Synonyms

100194131, AtDFB, cFPGS, DHFS/FPGS, dihydrofolate synthase/folylpolyglutamate synthase, eFPGS, Folate polyglutamate synthetase, FolC, folyl-gamma-glutamate synthetase, Folylpoly(.gamma.-glutamate) synthase, Folylpoly-.gamma.-glutamate synthase, Folylpoly-gamma-glutamate synthetase, Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase, Folylpolyglutamate synthase, Folylpolyglutamate synthetase, folylpolyglutamate synthetase 1, Folylpolyglutamyl synthetase, folypoly-gamma-glutamate synthetase, folypolyformyl glutamate synthase, folypolyglutamate synthase, folypolyglutamate synthetase, Formyltetrahydropteroyldiglutamate synthetase, FPGS, FPGS1, FPGS2, GRMZM2G393334, mFGPS, mFPGS, More, MTHFD1L, N10-Formyltetrahydropteroyldiglutamate synthetase, PfDHFS-FPGS, Synthetase, folylpolyglutamate, Tail length regulator, tetrahydrofolate:L-glutamate gamma-ligase (ADP-forming), tetrahydrofolylpolyglutamate synthase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.17 tetrahydrofolate synthase

Storage Stability

Storage Stability on EC 6.3.2.17 - tetrahydrofolate synthase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or -196°C, 50 mM potassium phosphate buffer, pH 7, 50 mM KCl, 30% DMSO
-
-20°C, stable for 3 weeks
-
-20ºC, 0.05% n-octylglucoside, DTT 2 mM, 50% glycerol, stable for 9 months. Replenishment of DTT every 2 months is necesary.
-
-20ºC, buffered 50% glycerol, quite stable
-
-25ºC, 50% glycerol, stable
-
-70ºC, 20% DMSO, stable
0-4°C, stable for several days, or at -20°C for longer periods
-
0°C, in phosphate-mercaptoethanol, pH 7.5, 75% of activity is retained after 10 days, 55% after 3 weeks
-
0ºC, leupeptin, MgATP2- 2 mM and 2-mercaptoethanol 50 mM or DTT 2 mM, limited stability. More stable with DTT in place of 2-mercaptoethanol.
-
20ºC, Tris buffer, without thiols, 85% activity over 30 min.
-
37ºC, Tris buffer, without thiols, with or without MgATP2- 5 mM, highly unstable, half-life of 6-7.3 min. Addition of 2-mercaptoethanol increases half-life of the enzyme to 11-21 min. Addition of 0.3 mg/ml BSA, stabilizes for 2 hours.
-
partially stabilized in presence of glycerol
-
purified enzyme loses activity within 1-2 days at 4, without stabilizer
-
stable at 0-4°C or at -20°C
-