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Literature summary for 6.3.2.17 extracted from

  • Shane, B.
    Pteroylpoly(gamma-glutamate) synthesis by Corynebacterium species. Purification and properties of folylpoly(gamma-glutamate) synthetase (1980), J. Biol. Chem., 255, 5655-5662.
    View publication on PubMed
Search for more literature for 6.3.2.17

Inhibitors

Inhibitors Comment Organism Structure
5,6,7,8-Tetrahydropteroate 5,6,7,8-tetrahydropteroyl-Glu2 formation Corynebacterium sp.
7,8-dihydropteroate 5,6,7,8-tetrahydropteroyl-Glu2 formation Corynebacterium sp.
7,8-dihydropteroyl-Glu 5,6,7,8-tetrahydropteroyl-Glu2 formation Corynebacterium sp.
ADP
-
 Corynebacterium sp.
aminopterin 5,6,7,8-tetrahydropteroyl-Glu2 formation Corynebacterium sp.
AMP
-
 Corynebacterium sp.
beta,gamma-methylene-ATP
-
 Corynebacterium sp.
phosphate
-
 Corynebacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 ATP
-
 Corynebacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ absolutely dependent on monovalent cation, K+ is most effective Corynebacterium sp.
K+ maximal activity at 200 mM Corynebacterium sp.
NH4+ stimulates to a lesser extent than K+ Corynebacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 gel filtration Corynebacterium sp.
53000
-
 1 * 53000, SDS-PAGE Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Corynebacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.57
-
-
 Corynebacterium sp.

Storage Stability

Storage Stability Organism
-20°C or -196°C, 50 mM potassium phosphate buffer, pH 7, 50 mM KCl, 30% DMSO Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Corynebacterium sp. ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 2 Corynebacterium sp. ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n:1 Corynebacterium sp. ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroate + Glu
-
 Corynebacterium sp. ADP + phosphate + 5,6,7,8-terahydropteroyl-Glu
-
 ?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Corynebacterium sp. ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + aminopterin + Glu
-
 Corynebacterium sp. ADP + phosphate + aminopteryl-Glu
-
 ?
CTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Corynebacterium sp. CDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Corynebacterium sp. dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
additional information pteroate and pteroylmonoglutamate substrates are utilized much more effectively than the polyglutamate derivatives. The most effective substrates are: 5,6,7,8-tetrahydropteroate, tetrahydrofolate, 5,10-methylene-tetrahydrofolate. The most effective diglutamate substrate is : 5,10-methylene-5,6,7,8-tetrahydropteroyldiglutamate Corynebacterium sp. ?
-
 ?
UTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Corynebacterium sp. UDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 53000, SDS-PAGE Corynebacterium sp.