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6.3.2.17: tetrahydrofolate synthase

This is an abbreviated version!
For detailed information about tetrahydrofolate synthase, go to the full flat file.

Word Map on EC 6.3.2.17

Reaction

ATP
+
tetrahydropteroyl-[gamma-Glu]n
 +
L-glutamate
=
ADP
 +
phosphate
 +
tetrahydropteroyl-[gamma-Glu]n+1

Synonyms

100194131, AtDFB, cFPGS, DHFS/FPGS, dihydrofolate synthase/folylpolyglutamate synthase, eFPGS, Folate polyglutamate synthetase, FolC, folyl-gamma-glutamate synthetase, Folylpoly(.gamma.-glutamate) synthase, Folylpoly-.gamma.-glutamate synthase, Folylpoly-gamma-glutamate synthetase, Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase, Folylpolyglutamate synthase, Folylpolyglutamate synthetase, folylpolyglutamate synthetase 1, Folylpolyglutamyl synthetase, folypoly-gamma-glutamate synthetase, folypolyformyl glutamate synthase, folypolyglutamate synthase, folypolyglutamate synthetase, Formyltetrahydropteroyldiglutamate synthetase, FPGS, FPGS1, FPGS2, GRMZM2G393334, mFGPS, mFPGS, More, MTHFD1L, N10-Formyltetrahydropteroyldiglutamate synthetase, PfDHFS-FPGS, Synthetase, folylpolyglutamate, Tail length regulator, tetrahydrofolate:L-glutamate gamma-ligase (ADP-forming), tetrahydrofolylpolyglutamate synthase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.17 tetrahydrofolate synthase

Specific Activity

Specific Activity on EC 6.3.2.17 - tetrahydrofolate synthase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000001
-
patients with acute myeloid leukemia, AML
0.00000006
-
enzyme from L44 cells
0.000000099
-
patients with T-linage acute lymphoblastic leukemia, T-ALL
0.0000001
0.000000244
-
patients with c/preB-acute lymphoblastic leukemia, c/preB-ALL
0.000001
-
YK01 methionine auxotoph mutant, 5,10-methylene-tetrahydrofolate as a substrate
0.0000015
-
YK01 methionine auxotoph mutant, tetrahydrofolate as a substrate
0.000008
-
wild type enzyme
0.00002458
-
K-562 cells
0.00002625
-
CCRF-CEM cells
0.00003516
-
A-253 cells
0.0000545
-
FaDu cells
0.0003
-
mutant E143A, pH 9.75
0.0005
-
mutant E143Q, pH 9.75
0.00055
-
mutant E143D, pH 9.75
0.0015
K185A mutant
0.0043
S412A mutant
0.0083
-
supernatant of cell extract
0.016
-
ammonium sulfate fraction
0.13
-
Sephacryl S-100 HR fraction
0.138
0.16
-
D345C mutant, pH 9.75
0.176
-
double mutant enzyme, pH 9.75
0.18
-
K172C mutant, pH 9.75
0.22
-
DEAE-Sephacel fraction
0.31
-
-
0.376
0.465
-
-
0.85
-
-
0.912
11.8
substrate 5,10-methylene-5,6,7,8-tetrahydrofolic acid, pH 9.8
13.2
substrate 5,10-methylene-5,6,7,8-tetrahydrofolic acid, pH 9.8
4.1
substrate 7,8-dihydropteroate, pH 9.8
45.6
-
-
5.2
-
-
additional information