Protein Variants | Comment | Organism |
---|---|---|
D345C | more than 85% of the wild type activity, with 5,10-methylene-5,6,7,8-tetrahydrofolic acid as substrate | Lacticaseibacillus casei |
K172C | more than 85% of the wild type activity, with 5,10-methylene-5,6,7,8-tetrahydrofolic acid as substrate | Lacticaseibacillus casei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
5,10-methylene-5,6,7,8-tetrahydrofolic acid | pH 9.75 | Lacticaseibacillus casei | |
1 | - |
(6RS)-5,6,7,8-tetrahydrofolic acid | pH 9.75 | Lacticaseibacillus casei | |
3 | - |
ATP | pH 9.75 | Lacticaseibacillus casei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + tetrahydrofolate-Glu + L-glutamate | Lacticaseibacillus casei | - |
ADP + phosphate + tetrahydrofolyl-Glu2 | - |
? | |
additional information | Lacticaseibacillus casei | the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
D345C mutant, pH 9.75 | Lacticaseibacillus casei |
0.176 | - |
double mutant enzyme, pH 9.75 | Lacticaseibacillus casei |
0.18 | - |
K172C mutant, pH 9.75 | Lacticaseibacillus casei |
0.2 | - |
wild type, pH 9.75 | Lacticaseibacillus casei |
Storage Stability | Organism |
---|---|
-70ºC, 20% DMSO, stable | Lacticaseibacillus casei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (6RS)-5,6,7,8-tetrahydrofolic acid + L-glutamate | not as effective as 5,10-methylene-5,6,7,8-tetrahydrofolic acid | Lacticaseibacillus casei | ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-Glu | - |
? | |
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate | - |
Lacticaseibacillus casei | ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu | - |
? | |
ATP + tetrahydrofolate-Glu + L-glutamate | - |
Lacticaseibacillus casei | ADP + phosphate + tetrahydrofolyl-Glu2 | - |
? | |
additional information | the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell | Lacticaseibacillus casei | ? | - |
? |