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6.3.2.17: tetrahydrofolate synthase

This is an abbreviated version!
For detailed information about tetrahydrofolate synthase, go to the full flat file.

Word Map on EC 6.3.2.17

Reaction

ATP
+
tetrahydropteroyl-[gamma-Glu]n
+
L-glutamate
=
ADP
+
phosphate
+
tetrahydropteroyl-[gamma-Glu]n+1

Synonyms

100194131, AtDFB, cFPGS, DHFS/FPGS, dihydrofolate synthase/folylpolyglutamate synthase, eFPGS, Folate polyglutamate synthetase, FolC, folyl-gamma-glutamate synthetase, Folylpoly(.gamma.-glutamate) synthase, Folylpoly-.gamma.-glutamate synthase, Folylpoly-gamma-glutamate synthetase, Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase, Folylpolyglutamate synthase, Folylpolyglutamate synthetase, folylpolyglutamate synthetase 1, Folylpolyglutamyl synthetase, folypoly-gamma-glutamate synthetase, folypolyformyl glutamate synthase, folypolyglutamate synthase, folypolyglutamate synthetase, Formyltetrahydropteroyldiglutamate synthetase, FPGS, FPGS1, FPGS2, GRMZM2G393334, mFGPS, mFPGS, More, MTHFD1L, N10-Formyltetrahydropteroyldiglutamate synthetase, PfDHFS-FPGS, Synthetase, folylpolyglutamate, Tail length regulator, tetrahydrofolate:L-glutamate gamma-ligase (ADP-forming), tetrahydrofolylpolyglutamate synthase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.17 tetrahydrofolate synthase

Purification

Purification on EC 6.3.2.17 - tetrahydrofolate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation and chromatography on BioGel A-0.5M, recombinant enzyme
-
ammonium sulfate fractionation, chromatography on Sephacryl S-100 and DEAE-Sephacel columns
-
ammonium sulfate fractionation, chromatography on Sephacryl S-100HR and DEAE-Sephacel ion exchange column, 25fold purification
-
ammonium sulfate precipitation, gel filtration and ion-exchange chromatography
-
ammonium sulfate precipitation, Sephacryl S-100/HR and DEAE-Sephacel chromatography, recombinant enzymes
-
chitin column chromatography
chromatography on a chitin column
crude total cell extracts are obtained by sonication followed by centrifugation
-
cytosolic, chloroplast and mitochondrial fractions
-
DE-52 cellulose column chromatography
-
differential centrifugation and ultracentrifugation on Nycodenz gradients, partial purification
-
liver, partial
-
Ni-Sepharose 6 Fastflow resin chromatography and Superdex-200 gel filtration
-
partial
partial purification
-
two forms
-