Literature summary for 6.3.2.17 extracted from

Bognar, A.L.; Shane, B.
Purification and properties of Lactobacillus casei folylpoly-gamma-glutamate synthetase (1983), J. Biol. Chem., 258, 12574-12581.

Search for more literature for 6.3.2.17

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	noncompetitive with respect to MgATP2-	Lacticaseibacillus casei
beta,gamma-methylene-ATP	-	Lacticaseibacillus casei
phosphate	competitive with respect to MgATP2-	Lacticaseibacillus casei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0023	-	5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2	-	Lacticaseibacillus casei
0.423	-	L-Glu	-	Lacticaseibacillus casei
5.6	-	ATP	-	Lacticaseibacillus casei

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	absolutely dependent on monovalent cation, K+ is most effective	Lacticaseibacillus casei
K+	maximal activity at 200 mM	Lacticaseibacillus casei
Mg2+	MgATP2- is an effective substrate	Lacticaseibacillus casei
Mn2+	MnATP2- is an effective substrate	Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	-	1 * 43000, SDS-PAGE	Lacticaseibacillus casei

Organism

Organism	UniProt	Comment	Textmining
Lacticaseibacillus casei	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Lacticaseibacillus casei

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate = ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1	ordered ter ter mechanism with MgATP binding first to the enzyme, folate second, and glutamate last	Lacticaseibacillus casei	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.23	-	-	Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (6R)-5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate	ATP in form of MgATP2-	Lacticaseibacillus casei	ADP + phosphate + (6R)-5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2	-	?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate	-	Lacticaseibacillus casei	ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu	-	?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu	n: 3	Lacticaseibacillus casei	ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1	-	?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu	n: 2	Lacticaseibacillus casei	ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1	-	?
dATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + Glu	-	Lacticaseibacillus casei	dADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2	-	?
GTP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu + Glu	-	Lacticaseibacillus casei	GDP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2	-	?
ITP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + Glu	-	Lacticaseibacillus casei	IDP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2	-	?
UTP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + Glu	-	Lacticaseibacillus casei	UDP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 43000, SDS-PAGE	Lacticaseibacillus casei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Lacticaseibacillus casei

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
10	-	-	Lacticaseibacillus casei

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Release 2023.1 (January 2023)