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3.1.4.3: phospholipase C

This is an abbreviated version!
For detailed information about phospholipase C, go to the full flat file.

Word Map on EC 3.1.4.3

Reaction

a phosphatidylcholine
+
H2O
=
1,2-diacyl-sn-glycerol
 +
phosphocholine

Synonyms

alpha-toxin, Beta toxin, Beta-hemolysin, Beta-toxin, broad-range phospholipase C, Cbp, CegC1, cholinespecific glycerophosphodiester phosphodiesterase, Clostridium oedematiens beta- and gamma-toxins, Clostridium welchii alpha-toxin, cPC-PLC, CpPLC, Dot/Icm-injected effector, Gamma-toxin, haemolytic phospholipase C, Heat labile-hemolysin, heat-labile hemolysin, Hemolysin, hemolytic PLC, hNPP6, Lecithinase, lecithinase C, lipophosphodiesterase C, lipophosphodiesterase I, Lpg0012, mNPP6, More, mPC-PLC, MTP40 antigen, non-specific phospholipase C, nonhemolytic PLC, nonspecific phospholipase C, NPC, NPC1, NPC1a, NPC1b, NPC2, NPC2a, NPC2b, NPC3, NPC3a, NPC3b, NPC4, NPC5, NPC6, NPC6a, NPC6b, NPC6c, NPC6d, NPP6, nucleotide pyrophosphatase/phosphodiesterase, PC-PLC, phosphatidase C, Phosphatidylcholine cholinephosphohydrolase, phosphatidylcholine phospholipase C, phosphatidylcholine specific phospholipase C, phosphatidylcholine-hydrolysing phospholipase C, phosphatidylcholine-hydrolyzing phospholipase C, phosphatidylcholine-preferring PLC, phosphatidylcholine-specific phospholipase C, phospholipase C, phospholipase C beta 1, phospholipase C gamma 1, phospholipase C gamma 2, phospholipase C/sphingomyelinase, phospholipase C/sphingomyelinase HR2, PLC, PLC-Bt, PLC-H, PLC-N, PLC1, PLC2, PlcB, PLCBC, PLCbeta, plcC, PlcC protein, PlcC/CegC1, PLCG1, PLCG2, PLCgamma, PlcHR2, PlcHR2 toxin, PLCLM, protein kinase C, PtdCho-PLC, Rv3487c, SMase, SMc00171, sphingomyelinase, type C phospholipase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.3 phospholipase C

Purification

Purification on EC 3.1.4.3 - phospholipase C

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography steps, column chromatography
-
affinity chromatography, gel filtration, 2500fold purification from culture filtrate
-
ammonium sulfate fractionation, ion-exchange chromatography, affinity chromatography, greater than 99% pure, 94fold
-
ammonium sulfate precipitation and chitin resin column chromatography
-
ammonium sulfate precipitation, column chromatography
-
ammonium sulfate precipitation, column chromatography steps
-
ammonium sulfate precipitation, ethanol precipitate, protamine sulfate supernatant, gel filtration, ion-exchange chromatography, 450fold, approximately 98% pure
-
anion-exchange chromatography, column chromatography, 342fold
-
by a nickel-nitrilotriacetic acid-agarose column
by serial ion exchange and gel filtration column chromatography
-
C-1and C-2: dialysis, isoelectric focusing and column chromatography, C-1: 1510foldfold, C-2: 1330fold, both to homogeneity
-
column chromatography
-
column chromatography, gel filtration
-
column chromatography, gel filtration chromatography, 190fold
-
column chromatography, ion-exchange chromatography
-
dialysis, column chromatography, 22fold
-
from culture supernatant
gel filtration, ion-exchange chromatography, 3645fold, apparently pure
-
ion-exchange chromatography, fractionation by manganous chloride, ammonium sulfate precipitation steps
-
method for production of recombinant enzyme in Schizosaccharomyces pombe
-
mono Q ion exchange chromatography column and eluted with a linear gradient of NaCl (0-2 M) using the deltaKTA system
native enzyme
-
partial
partial purification, ammonium sulfate precipitation
-
partial, cPC-PLC
-
partial, mPC-PLC
-
protamine precipitation, column chromatography, 23fold
-
purification of Gh-PLC complex, gel filtration, ion-exchange chromatography
-
recombinant PLC d1 and D3 with poly-His-tail at the amino terminus, metal ion affinity chromatography
-
recombinant PLC d1 and D3 without poly-His-tail at the amino terminus, cation exchange HPLC and phosphocellulose chromatography
-
recombinant PLC, cation exchange chromatography, 26fold to homogeneity
-
recombinant PLC, NiNTA, two FPLC steps, 160fold
-
recombinant PLC-beta 1a 58fold, recombinant PLC-beta 1b 47fold, HPLC, affinity chromatography, reverse phase chromatography
-
ultrafiltration, column chromatography, 792fold
-
wild-type and mutant H148G
-