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Literature summary for 3.1.4.3 extracted from

  • Chin, J.C.; Watts, J.E.
    Biological properties of phospholipase C purified from a fleecerot isolate of Pseudomonas aeruginosa (1988), J. Gen. Microbiol., 134, 2567-2575.
    View publication on PubMed

General Stability

General Stability Organism
the enzyme is unstable, it can be stabilized by addition of sorbitol to 60% w/w in 25 mM Tris/HCl buffer, pH 7.2 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa the enzyme, when injected intradermally into the skin of sheep, elicits histopathological lesions virtually identical to that seen in naturally occuring fleecerot ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48.7
-
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme, when injected intradermally into the skin of sheep, elicits histopathological lesions virtually identical to that seen in naturally occuring fleecerot Pseudomonas aeruginosa ?
-
?
p-nitrophenylphosphorylcholine + H2O
-
Pseudomonas aeruginosa p-nitrophenol + phosphorylcholine
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Pseudomonas aeruginosa