Literature summary for 3.1.4.3 extracted from

Lucchesi, G.I.; Domenech, C.E.
A simple and reliable method for the purification of Pseudomonas aeruginosa phospholipase C produced in a high phosphate medium containing choline (1994), Int. J. Biochem., 26, 155-162.

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Inhibitors

Inhibitors	Comment	Organism	Structure
Triton X-100	30% inhibition by 1% Triton X-100	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
25	-	p-Nitrophenylphosphorylcholine	37°C, pH 7.4	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	x * 70000, SDS-PAGE	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas aeruginosa

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture supernatant	-	Pseudomonas aeruginosa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
13.27	-	-	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenylphosphorylcholine + H2O	-	Pseudomonas aeruginosa	p-nitrophenol + phosphorylcholine	-	?

Subunits

Subunits	Comment	Organism
?	x * 70000, SDS-PAGE	Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
100	-	1 min, activity is destroyed	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)