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3.1.4.3: phospholipase C

This is an abbreviated version!
For detailed information about phospholipase C, go to the full flat file.

Word Map on EC 3.1.4.3

Reaction

a phosphatidylcholine
+
H2O
=
1,2-diacyl-sn-glycerol
 +
phosphocholine

Synonyms

alpha-toxin, Beta toxin, Beta-hemolysin, Beta-toxin, broad-range phospholipase C, Cbp, CegC1, cholinespecific glycerophosphodiester phosphodiesterase, Clostridium oedematiens beta- and gamma-toxins, Clostridium welchii alpha-toxin, cPC-PLC, CpPLC, Dot/Icm-injected effector, Gamma-toxin, haemolytic phospholipase C, Heat labile-hemolysin, heat-labile hemolysin, Hemolysin, hemolytic PLC, hNPP6, Lecithinase, lecithinase C, lipophosphodiesterase C, lipophosphodiesterase I, Lpg0012, mNPP6, More, mPC-PLC, MTP40 antigen, non-specific phospholipase C, nonhemolytic PLC, nonspecific phospholipase C, NPC, NPC1, NPC1a, NPC1b, NPC2, NPC2a, NPC2b, NPC3, NPC3a, NPC3b, NPC4, NPC5, NPC6, NPC6a, NPC6b, NPC6c, NPC6d, NPP6, nucleotide pyrophosphatase/phosphodiesterase, PC-PLC, phosphatidase C, Phosphatidylcholine cholinephosphohydrolase, phosphatidylcholine phospholipase C, phosphatidylcholine specific phospholipase C, phosphatidylcholine-hydrolysing phospholipase C, phosphatidylcholine-hydrolyzing phospholipase C, phosphatidylcholine-preferring PLC, phosphatidylcholine-specific phospholipase C, phospholipase C, phospholipase C beta 1, phospholipase C gamma 1, phospholipase C gamma 2, phospholipase C/sphingomyelinase, phospholipase C/sphingomyelinase HR2, PLC, PLC-Bt, PLC-H, PLC-N, PLC1, PLC2, PlcB, PLCBC, PLCbeta, plcC, PlcC protein, PlcC/CegC1, PLCG1, PLCG2, PLCgamma, PlcHR2, PlcHR2 toxin, PLCLM, protein kinase C, PtdCho-PLC, Rv3487c, SMase, SMc00171, sphingomyelinase, type C phospholipase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.3 phospholipase C

KM Value

KM Value on EC 3.1.4.3 - phospholipase C

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
43.5
1,2-dibutyryl-sn-glycero-3-phosphocholine
-
at pH 6.0 and 37°C
8.5
1,2-diheptanoyl-sn-glycero-3-phosphocholine
-
at pH 6.0 and 37°C
4.5
1,2-dihexanoyl-sn-glycero-3-phospho-L-serine
pH 7.3
2.4 - 2.8
1,2-dihexanoyl-sn-glycero-3-phosphocholine
1.8
1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine
pH 7.3
0.166 - 0.6
1,2-dipalmitoylphosphatidylcholine
4.5 - 7
1,2-dipalmitoylphosphatidylethanolamine
-
-
0.563
1-arachidonoyl-lysophosphatidylcholine
Vmax 470 nmol/min mg
0.296
1-lauroyl-lysophosphatidylcholine
Vmax 463 nmol/min mg
0.434
1-linoleoyl-lysophosphatidylcholine
Vmax 285 nmol/min mg
0.44
1-myristoyl-lysophosphatidylcholine
Vmax 367 nmol/min mg
0.271
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
pH 5.0, 37°C
0.025 - 0.165
1-palmitoyl-lysophosphatidylcholine
14.8 - 39.5
4-nitrophenylphosphorylcholine
0.03 - 1.09
diheptanoylphosphatidylcholine
4.2 - 9
dihexanoylphosphatidic acid
0.19 - 1.12
dihexanoylphosphatidylcholine
0.344
Glycerophosphorylcholine
Vmax 484 nmol/min mg
0.0513
L-alpha-lysophosphatidylcholine
pH 5.0, 37°C
0.016 - 1.2
lecithin
-
-
14.1 - 200
p-Nitrophenylphosphorylcholine
0.38 - 0.9
phosphatidylcholine
0.087 - 0.369
phosphatidylinositol
0.137 - 0.571
phosphatidylinositol 4,5-bisphosphate
2 - 3
phosphatidylserine
-
-
0.09 - 0.8
sphingomyelin
additional information
additional information
-
kinetic parameters depend on the physicochemical properties of substrate aggregates. Larger micelles with a ratio bile salt/lipid smaller than 5 show higher activity and shorter steady state duration of less than 4 min, smaller micelles with a ratio bile salt/lipid greater than 5 show lower activity and longer steady state of about 10 min
-