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Literature summary for 3.1.4.3 extracted from
- Characterization of a phospholipase C activity regulated by the purified Gh in reconstitution systems (1992), J. Biol. Chem., 267, 8887-8894.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| guanine nucleotide-binding regulatory protein Gh | stimulating only in presence of GTPgammaS, lowers required Ca2+ concentration | Rattus norvegicus | |
| guanine nucleotide-binding regulatory protein Ghalpha | stimulation in presence of aluminium fluoride, lowers required Ca2+ concentration | Rattus norvegicus | |
| phosphatidylcholine | - |
Rattus norvegicus |  |
| phosphatidylserine | - |
Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activity rapidly decreases at concentrations of 0.015-0.030 mM and above | Rattus norvegicus | |
| Mg2+ | 1-3 mM, substrate in detergent solution | Rattus norvegicus | |
| phosphatidylethanolamine | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Rattus norvegicus | |
| Mg2+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylinositol-bisphosphate + H2O | Rattus norvegicus | - |
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of Gh-PLC complex, gel filtration, ion-exchange chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3 | 5 | substrate: phosphatidylinositol-4,5-bisphosphate | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, buffer containing 5% glycerol and 0,1% sucrose monolaureate, stable for less than 2 weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylinositol-bisphosphate + H2O | - |
Rattus norvegicus | inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? | |
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