Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sodium deoxycholate | substrate: egg yolk lipoprotein | Bacillus cereus |
General Stability | Organism |
---|---|
1,10-phenanthroline, denaturation | Bacillus cereus |
sodium dodecyl sulfate | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Bacillus cereus | |
EDTA | - |
Bacillus cereus | |
o-phenanthroline | - |
Bacillus cereus | |
SDS | - |
Bacillus cereus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus cereus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | restores activity after EDTA treatment | Bacillus cereus | |
Zn2+ | restores activity after EDTA treatment | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
gel filtration | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | Bacillus cereus | - |
1,2-diacylglycerol + phosphorylcholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, ethanol precipitate, protamine sulfate supernatant, gel filtration, ion-exchange chromatography, 450fold, approximately 98% pure | Bacillus cereus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1010 | - |
substrate: lecithin | Bacillus cereus |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 6 months, no loss of activity | Bacillus cereus |
20°C, 50% glycerol, 1 week, no significant loss of activity | Bacillus cereus |
20°C, 50% glycerol, after 1 week: 1% loss of activity per day | Bacillus cereus |
4°C, impure preparations, 77% saturated with (NH4)2SO4, stable | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cardiolipin + H2O | - |
Bacillus cereus | phosphatidylglycerophosphate + 1,2-diacylglycerol | - |
? | |
glucosaminylphosphatidylglycerol + H2O | - |
Bacillus cereus | ? | - |
? | |
phosphatidic acid + H2O | - |
Bacillus cereus | ? | - |
? | |
phosphatidylcholine + H2O | - |
Bacillus cereus | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
phosphatidylethanolamine + H2O | - |
Bacillus cereus | phosphorylethanolamine + diacylglycerol | - |
? | |
phosphatidylglycerol + H2O | - |
Bacillus cereus | 1,2-diacylglycerol + sn-glycerol-3-phosphate | - |
? | |
phosphatidylinositol + H2O | - |
Bacillus cereus | inositol monophosphate + diacylglycerol | - |
? | |
phosphatidylserine + H2O | - |
Bacillus cereus | phosphorylserine + diacylglycerol | - |
? | |
sphingomyelin + H2O | - |
Bacillus cereus | ceramide + phosphorylcholine | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
for 20 min, no significant loss of activity | Bacillus cereus |