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Literature summary for 3.1.4.3 extracted from

  • Zwaal, R.F.A.; Roelofsen, B.
    Phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) from Bacillus cereus (1974), Methods Enzymol., 32, 154-161.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
sodium deoxycholate substrate: egg yolk lipoprotein Bacillus cereus

General Stability

General Stability Organism
1,10-phenanthroline, denaturation Bacillus cereus
sodium dodecyl sulfate Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol
-
Bacillus cereus
EDTA
-
Bacillus cereus
o-phenanthroline
-
Bacillus cereus
SDS
-
Bacillus cereus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus cereus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ restores activity after EDTA treatment Bacillus cereus
Zn2+ restores activity after EDTA treatment Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
gel filtration Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Bacillus cereus
-
1,2-diacylglycerol + phosphorylcholine
-
?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, ethanol precipitate, protamine sulfate supernatant, gel filtration, ion-exchange chromatography, 450fold, approximately 98% pure Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1010
-
substrate: lecithin Bacillus cereus

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, 6 months, no loss of activity Bacillus cereus
20°C, 50% glycerol, 1 week, no significant loss of activity Bacillus cereus
20°C, 50% glycerol, after 1 week: 1% loss of activity per day Bacillus cereus
4°C, impure preparations, 77% saturated with (NH4)2SO4, stable Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cardiolipin + H2O
-
Bacillus cereus phosphatidylglycerophosphate + 1,2-diacylglycerol
-
?
glucosaminylphosphatidylglycerol + H2O
-
Bacillus cereus ?
-
?
phosphatidic acid + H2O
-
Bacillus cereus ?
-
?
phosphatidylcholine + H2O
-
Bacillus cereus 1,2-diacylglycerol + phosphorylcholine
-
?
phosphatidylethanolamine + H2O
-
Bacillus cereus phosphorylethanolamine + diacylglycerol
-
?
phosphatidylglycerol + H2O
-
Bacillus cereus 1,2-diacylglycerol + sn-glycerol-3-phosphate
-
?
phosphatidylinositol + H2O
-
Bacillus cereus inositol monophosphate + diacylglycerol
-
?
phosphatidylserine + H2O
-
Bacillus cereus phosphorylserine + diacylglycerol
-
?
sphingomyelin + H2O
-
Bacillus cereus ceramide + phosphorylcholine
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
for 20 min, no significant loss of activity Bacillus cereus