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3.1.4.3: phospholipase C

This is an abbreviated version!
For detailed information about phospholipase C, go to the full flat file.

Word Map on EC 3.1.4.3

Reaction

a phosphatidylcholine
+
H2O
=
1,2-diacyl-sn-glycerol
+
phosphocholine

Synonyms

alpha-toxin, Beta toxin, Beta-hemolysin, Beta-toxin, broad-range phospholipase C, Cbp, CegC1, cholinespecific glycerophosphodiester phosphodiesterase, Clostridium oedematiens beta- and gamma-toxins, Clostridium welchii alpha-toxin, cPC-PLC, CpPLC, Dot/Icm-injected effector, Gamma-toxin, haemolytic phospholipase C, Heat labile-hemolysin, heat-labile hemolysin, Hemolysin, hemolytic PLC, hNPP6, Lecithinase, lecithinase C, lipophosphodiesterase C, lipophosphodiesterase I, Lpg0012, mNPP6, More, mPC-PLC, MTP40 antigen, non-specific phospholipase C, nonhemolytic PLC, nonspecific phospholipase C, NPC, NPC1, NPC1a, NPC1b, NPC2, NPC2a, NPC2b, NPC3, NPC3a, NPC3b, NPC4, NPC5, NPC6, NPC6a, NPC6b, NPC6c, NPC6d, NPP6, nucleotide pyrophosphatase/phosphodiesterase, PC-PLC, phosphatidase C, Phosphatidylcholine cholinephosphohydrolase, phosphatidylcholine phospholipase C, phosphatidylcholine specific phospholipase C, phosphatidylcholine-hydrolysing phospholipase C, phosphatidylcholine-hydrolyzing phospholipase C, phosphatidylcholine-preferring PLC, phosphatidylcholine-specific phospholipase C, phospholipase C, phospholipase C beta 1, phospholipase C gamma 1, phospholipase C gamma 2, phospholipase C/sphingomyelinase, phospholipase C/sphingomyelinase HR2, PLC, PLC-Bt, PLC-H, PLC-N, PLC1, PLC2, PlcB, PLCBC, PLCbeta, plcC, PlcC protein, PlcC/CegC1, PLCG1, PLCG2, PLCgamma, PlcHR2, PlcHR2 toxin, PLCLM, protein kinase C, PtdCho-PLC, Rv3487c, SMase, SMc00171, sphingomyelinase, type C phospholipase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.3 phospholipase C

Storage Stability

Storage Stability on EC 3.1.4.3 - phospholipase C

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or 4°C, 0.3 M triethanolamine or 0.05 M Tris-HCl, pH 7, stable for up to 12 months
-
-20°C, 1-2 mg purified enzyme/ml, indefinitely stable
-
-20°C, 100 U/ml distilled water, stable for at least 6 months
-
-20°C, 50% glycerol, 6 months, no loss of activity
-
-20°C, 70 mg crude enzyme/ml, stable for at least a year
-
-20°C, buffer B, purified recombinant PLC d1 and d3 with poly-His-tail, activity stable over 2 years, more than 15 freeze/thaw cycles between -20°C and 4°C reduce activity by 10-20%
-
-20°C, crude enzyme, stable for more than 6 months
-
-20°C, crude extract, enzyme activity stable over 2 years
-
-20°C, in barbital-buffered saline, pH 7.4, 1 mM ZnSO4, stable for more than one year
-
-20°C, in Na2B4O7/HCl buffer, pH 7.5, 40% glycerol, no loss of activity after 6 months
-
-20°C, more than 1-2 mg purified enzyme/ml, stable for at least 3 months
-
-20°C, stable for more than 6 months, crude enzyme
-
-60°C, for one year, activity only slightly decreased
-
-70°C
-
-70°C, buffer containing 5% glycerol and 0,1% sucrose monolaureate, stable for less than 2 weeks
-
-80°C
20°C, 50% glycerol, 1 week, no significant loss of activity
-
20°C, 50% glycerol, after 1 week: 1% loss of activity per day
-
4°C, 100 U/ml distilled water, stable for several days
-
4°C, full activity after 1-2 months
-
4°C, impure preparations, 77% saturated with (NH4)2SO4, stable
-
4°C, lyophilized crude preparation, stable for at least 6 months
-
frozen, neutral pH, almost full activity after 2 years
-