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Literature summary for 3.1.4.3 extracted from
- Recombinant broad-range phospholipase C from Listeria monocytogenes exhibits optimal activity at acidic pH (2016), Biochim. Biophys. Acta, 1864, 697-705 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-AI cells | Listeria monocytogenes |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C143S | the mutant shows increased activity compared to the wild type enzyme | Listeria monocytogenes |
| C168S | the mutant shows decreased activity compared to the wild type enzyme | Listeria monocytogenes |
| D55N | the mutation causes a dramatic loss of activity | Listeria monocytogenes |
| F66Y | the mutation causes a dramatic loss of activity | Listeria monocytogenes |
| H56Y | the activity of the mutant is comparable to the wild type enzyme | Listeria monocytogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
1,2-dihexanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes |  |
| 8.5 | - |
1,2-diheptanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
| 43.5 | - |
1,2-dibutyryl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | a zinc metalloenzyme, 0.05 mM used in assay conditions | Listeria monocytogenes | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | Listeria monocytogenes | - |
1,2-diacyl-sn-glycerol + phosphorylcholine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Listeria monocytogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and chitin resin column chromatography | Listeria monocytogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dibutyryl-sn-glycero-3-phosphocholine + H2O | - |
Listeria monocytogenes | 1,2-dibutyryl-sn-glycerol + phosphocholine | - |
? | |
| 1,2-diheptanoyl-sn-glycero-3-phosphocholine + H2O | - |
Listeria monocytogenes | 1,2-diheptanoyl-sn-glycerol + phosphocholine | - |
? | |
| 1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O | - |
Listeria monocytogenes | 1,2-dihexanoyl-sn-glycerol + phosphocholine | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) + H2O | about 25% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine | Listeria monocytogenes | 1,2-dioleoyl-sn-glycerol + glycerol 1-phosphate | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-phospho-L-serine + H2O | 60% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine | Listeria monocytogenes | 1,2-dioleoyl-sn-glycerol + phospho-L-serine | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-phosphocholine + H2O | 100% activity | Listeria monocytogenes | 1,2-dioleoyl-sn-glycerol + phosphocholine | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine + H2O | about 70% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine | Listeria monocytogenes | 1,2-dioleoyl-sn-glycerol + phosphoethanolamine | - |
? | |
| additional information | no activity with phosphatidylinositol | Listeria monocytogenes | ? | - |
? | |
| phosphatidylcholine + H2O | - |
Listeria monocytogenes | 1,2-diacyl-sn-glycerol + phosphorylcholine | - |
? | |
| sphingomyelin + H2O | about 70% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine | Listeria monocytogenes | ceramide + phosphocholine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, proenzyme, SDS-PAGE | Listeria monocytogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| broad-range phospholipase C | - |
Listeria monocytogenes |
| PLC | - |
Listeria monocytogenes |
| PlcB | - |
Listeria monocytogenes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
1,2-dibutyryl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
| 8.5 | - |
1,2-diheptanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
| 12.8 | - |
1,2-dihexanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Listeria monocytogenes |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
1,2-dibutyryl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
| 4.6 | - |
1,2-dihexanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
| 42 | - |
1,2-diheptanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
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