Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.3 extracted from

  • Fox, J.A.; Duszenko, M.; Ferguson, M.A.J.; Low, M.G.; Cross, G.A.M.
    Purification and characterization of a novel glycan-phosphatidylinositol-specific phospholipase C from Trypanosoma brucei (1986), J. Biol. Chem., 261, 15767-15771.
    View publication on PubMed
Search for more literature for 3.1.4.3

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
 Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Trypanosoma brucei
Hg2+
-
 Trypanosoma brucei
Zn2+
-
 Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Trypanosoma brucei 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 SDS-PAGE Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycan-phosphatidylinositol + H2O Trypanosoma brucei
-
 ?
-
 ?
glycan-phosphatidylinositol + H2O Trypanosoma brucei 427
-
 ?
-
 ?
membrane form variant surface glycoproteins + H2O Trypanosoma brucei
-
 soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol
-
 ?
membrane form variant surface glycoproteins + H2O Trypanosoma brucei 427
-
 soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion-exchange chromatography, column chromatography, 342fold Trypanosoma brucei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00245
-
 substrate: membrane form variant surface glycoproteins Trypanosoma brucei

Storage Stability

Storage Stability Organism
4°C, full activity after 1-2 months Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycan-phosphatidylinositol + H2O
-
 Trypanosoma brucei ?
-
 ?
glycan-phosphatidylinositol + H2O
-
 Trypanosoma brucei ?
-
 r
glycan-phosphatidylinositol + H2O
-
 Trypanosoma brucei 427 ?
-
 ?
glycan-phosphatidylinositol + H2O
-
 Trypanosoma brucei 427 ?
-
 r
membrane form variant surface glycoproteins + H2O
-
 Trypanosoma brucei soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol
-
 ?
membrane form variant surface glycoproteins + H2O
-
 Trypanosoma brucei 427 soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol
-
 ?