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3.1.4.3: phospholipase C

This is an abbreviated version!
For detailed information about phospholipase C, go to the full flat file.

Word Map on EC 3.1.4.3

Reaction

a phosphatidylcholine
+
H2O
=
1,2-diacyl-sn-glycerol
 +
phosphocholine

Synonyms

alpha-toxin, Beta toxin, Beta-hemolysin, Beta-toxin, broad-range phospholipase C, Cbp, CegC1, cholinespecific glycerophosphodiester phosphodiesterase, Clostridium oedematiens beta- and gamma-toxins, Clostridium welchii alpha-toxin, cPC-PLC, CpPLC, Dot/Icm-injected effector, Gamma-toxin, haemolytic phospholipase C, Heat labile-hemolysin, heat-labile hemolysin, Hemolysin, hemolytic PLC, hNPP6, Lecithinase, lecithinase C, lipophosphodiesterase C, lipophosphodiesterase I, Lpg0012, mNPP6, More, mPC-PLC, MTP40 antigen, non-specific phospholipase C, nonhemolytic PLC, nonspecific phospholipase C, NPC, NPC1, NPC1a, NPC1b, NPC2, NPC2a, NPC2b, NPC3, NPC3a, NPC3b, NPC4, NPC5, NPC6, NPC6a, NPC6b, NPC6c, NPC6d, NPP6, nucleotide pyrophosphatase/phosphodiesterase, PC-PLC, phosphatidase C, Phosphatidylcholine cholinephosphohydrolase, phosphatidylcholine phospholipase C, phosphatidylcholine specific phospholipase C, phosphatidylcholine-hydrolysing phospholipase C, phosphatidylcholine-hydrolyzing phospholipase C, phosphatidylcholine-preferring PLC, phosphatidylcholine-specific phospholipase C, phospholipase C, phospholipase C beta 1, phospholipase C gamma 1, phospholipase C gamma 2, phospholipase C/sphingomyelinase, phospholipase C/sphingomyelinase HR2, PLC, PLC-Bt, PLC-H, PLC-N, PLC1, PLC2, PlcB, PLCBC, PLCbeta, plcC, PlcC protein, PlcC/CegC1, PLCG1, PLCG2, PLCgamma, PlcHR2, PlcHR2 toxin, PLCLM, protein kinase C, PtdCho-PLC, Rv3487c, SMase, SMc00171, sphingomyelinase, type C phospholipase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.3 phospholipase C

Cloned

Cloned on EC 3.1.4.3 - phospholipase C

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana
-
expressed in Escherichia coli BL21-AI cells
-
expressed in Pichia pastoris strain GS115
-
expressed in Pichia pastoris strain GS115H
-
expression in Bacillus subtilis
-
expression in Escherichia coli
expression in Escherichia coli K-12
-
expression in Mycobacterium smegmatis
expression of alpha-toxin in Escherichia coli
-
expression of the N-terminal domain in Escherichia coli
-
functional SMc00171 expression in Escherichia coli
-
gene lpg0012 or plcC, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21, subcloning in Escherichia coli strain DH5alpha. Expression of Strep-tagged enzyme mutants H179N and S336A does not yield any detectable protein
into pBluescript KS, for sequencing
-
mpcA and mpcB are individually cloned in Mycobacterium smegmatis
nonhemolytic PLC
-
PCR product cloned using the original TA cloning Kit, subcloned into the pCR2.1 vector
phylogenetic analysis
production of the recombinant protein in Escherichia coli is no more successful because of frequent inclusion bodies and accumulation of inactive phospholipase C in the periplasmic space
-
tranformed in Escherichia coli
-
transfected into CV-1 cells
-
transformed into Escherichia coli
-