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Literature summary for 3.1.4.3 extracted from

  • Durban, M.A.; Silbersack, J.; Schweder, T.; Schauer, F.; Bornscheuer, U.T.
    High level expression of a recombinant phospholipase C from Bacillus cereus in Bacillus subtilis (2007), Appl. Microbiol. Biotechnol., 74, 634-639.
    View publication on PubMed
Search for more literature for 3.1.4.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis Bacillus cereus

Protein Variants

Protein Variants Comment Organism
additional information amino acid sequence differs from that of strain SBUG516 by substitution D173E Bacillus cereus
additional information amino acid sequence differs from that of strain SBUG516 by substitution E173D Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus SBUG318
-
-
-
Bacillus cereus SBUG516
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13190
-
 37°C Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenylphosphorylcholine + H2O
-
 Bacillus cereus p-nitrophenol + phosphorylcholine
-
 ?
p-nitrophenylphosphorylcholine + H2O
-
 Bacillus cereus SBUG516 p-nitrophenol + phosphorylcholine
-
 ?
p-nitrophenylphosphorylcholine + H2O
-
 Bacillus cereus SBUG318 p-nitrophenol + phosphorylcholine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bacillus cereus