Crystallization (Comment) | Organism |
---|---|
- |
Bacillus cereus |
General Stability | Organism |
---|---|
1,10-phenanthroline, denaturation | Bacillus cereus |
urea, PLC is catalytically active in 8 M urea | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | in absence of free Zn2+ in solution | Bacillus cereus | |
Cu2+ | in absence of free Zn2+ in solution | Bacillus cereus | |
EDTA | - |
Bacillus cereus | |
Ni2+ | in absence of free Zn2+ in solution | Bacillus cereus | |
o-phenanthroline | - |
Bacillus cereus | |
Urea | denaturation | Bacillus cereus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus cereus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | metalloenzyme | Bacillus cereus | |
Zn2+ | 2 mol Zn2+ per mol enzyme | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
ultracentrifugation, SDS-PAGE, amino acid calculation | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | Bacillus cereus | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
sphingomyelin + H2O | Bacillus cereus | - |
ceramide + phosphorylcholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
strain ATCC 10987 AB-1 | - |
Purification (Comment) | Organism |
---|---|
affinity chromatography steps, column chromatography | Bacillus cereus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1510 | - |
- |
Bacillus cereus |
Storage Stability | Organism |
---|---|
-20°C, 1-2 mg purified enzyme/ml, indefinitely stable | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | - |
Bacillus cereus | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
phosphatidylethanolamine + H2O | - |
Bacillus cereus | phosphorylethanolamine + diacylglycerol | - |
? | |
phosphatidylinositol + H2O | - |
Bacillus cereus | inositol monophosphate + diacylglycerol | - |
? | |
phosphatidylserine + H2O | - |
Bacillus cereus | phosphorylserine + diacylglycerol | - |
? | |
sphingomyelin + H2O | - |
Bacillus cereus | ceramide + phosphorylcholine | - |
? | |
sphingomyelin + H2O | when substrate is presented in a biological membrane, for example in the human red cell membrane or myelin sheath | Bacillus cereus | ceramide + phosphorylcholine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 23000, ultracentrifugation, SDS-PAGE, amino acid calculation | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
substrate: insoluble long-chain phosphatidylcholine | Bacillus cereus |
7.5 | 8 | substrate: water-soluble short-chain phosphatidylcholine | Bacillus cereus |
8 | - |
substrate: insoluble long-chain phosphatidylcholine | Bacillus cereus |