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3.1.1.73: feruloyl esterase

This is an abbreviated version!
For detailed information about feruloyl esterase, go to the full flat file.

Word Map on EC 3.1.1.73

Reaction

feruloyl-polysaccharide
+
H2O
=
ferulate
 +
Polysaccharide

Synonyms

4-hydroxy-3-methoxycinnamic acid esterase, A.O.1, A.O.11, A.O.12, A.O.13, A.O.2, A.O.3, A.O.4, A.O.5, A.O.6, A.O.7, A.O.8, A.O.9, acetyl/ferulic acid esterase, AfFaeA, AN1772.2, AnFAE, AnFaeA, AnFAEB, AO090701000884, AoFaeB, AoFaeC, AtFAE-1, AtFAE-2, AtFAE-3, AuFaeA, AusFaeA, AwFAE, AwFAEA, BioH, carboxylic ester hydrolase, CE1, cellulosome multi-enzyme complex, cellulosome xylanase Z feruloyl esterase, CinI, CinII, cinnAE, cinnamic acid esterase, cinnamic acid hydrolase, cinnamic acid hydrolases, cinnamoyl ester hydrolase, Cinnamoyl esterase, CjXYLD, Est1, Est1E, EstA, esterase A, EstF27, FA esterase, FAE, FAE B, Fae-1, FAE-2, FAE-A, FAE-B, FAE-C, FAE-D, FAE-I, FAE-II, FAE-III, FAE-PL, FAE1, FAE125, Fae1A, FAE2, FAE3, FAE4, FAE5, FAE6, FAE68, FAE7, FAE7262, FAEA, FAEA1, FAEA2, FAEB, FaeB1, FAEB2, FaeC, FaeD, FaeD-3.544, FaeD1, FaeD2, FaeI, FaeT, FAE_XynZ, FE, FeE, Fee1B, ferulic acid esterase, ferulic acid esterase A, ferulic acid esterase B, ferulic acid esterase C, ferulic acid esterase D, feruloyl esterase, feruloyl esterase A, feruloyl esterase B, feruloyl/p-coumaroyl esterase, Feruloylesterase, FoFAE, FoFAE-I, FoFAE-II, FoFaeA, FoFaeB, FoFaeI, FoFaeII, hemicellulase acessory enzymes, hydroxycinnamoyl esterase, KX091144, Lp_0796, More, NcFaeB, NcFaeD-3.544, PeFaeA, PfFaeB, phenolic acid esterase, PSHAa enzyme, PSHAa1385, R18, SCHCODRAFT_60993, SCHCODRAFT_61055, StFAE, StFAE-A, StFaeB, StFaeC, TH2-18, TsFaeA, TsFaeB, TsFaeC, Tvms10a, Tvmz2a, type A FAE, type A feruloyl esterase, type B FAE, type B ferulic acid esterase, XLYD, XM 001217492, xylan-degrading enzyme system, xylanase 10, xylanase 10B, xylanase Z, xylanase-ferulic acid esterase, XYLD, XYLD esterase, Xyn10B, Xyn10D-Fae1A, XynZ

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.73 feruloyl esterase

Specific Activity

Specific Activity on EC 3.1.1.73 - feruloyl esterase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0004
-
with 1 mM p-nitrophenyl palmitate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0
0.005
-
about, recombinant feruoyl esterase FaeD, pH 7.5, 37°C, substrate 4-nitrophenyl acetate
0.0083
-
about, recombinant feruoyl esterase FaeT, pH 7.5, 37°C, substrate 4-nitrophenyl acetate
0.0117
-
purified FAE-2, with 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl beta-D-mannopyranoside or 4-nitrophenyl beta-D-xylopyranoside as substrates
0.015
-
in submerged fermentations at 24 h
0.0167
-
purified FAE-1, with 4-nitrophenyl butyrate, 4-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-mannopyranoside as substrates
0.025
-
purified FAE-1, with 4-nitrophenyl alpha-L-arabinofuranoside or 4-nitrophenyl beta-D-xylopyranoside as substrates
0.0267
-
about, recombinant feruoyl esterase FaeD, pH 7.5, 37°C, substrate 4-nitrophenyl acetate, in presence of 0.5 mM ferulic acid
0.03
-
with 5 mM methyl vanillate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0
0.0317
-
purified FAE-2, with 4-nitrophenyl butyrate as substrate
0.033
-
about, recombinant feruoyl esterase FaeT, pH 7.5, 37°C, substrate 4-nitrophenyl acetate, in presence of 0.5 mM ferulic acid
0.05
-
with 5 mM methyl caffeate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0
0.0538
-
crude extract
0.102
-
purified FAE-2, with 4-nitrophenyl acetate as substrate
0.103
-
purified FAE-1, with 4-nitrophenyl acetate as substrate
0.15
-
purified FAE-1, with 4-nitrophenyl ferulate as substrate
0.1867
-
purified FAE-2, with 4-nitrophenyl ferulate as substrate
0.19
-
methyl ferulate as substrate
0.195
-
on day 3 in submerged fermentations, remains constant up to 5 days
0.213
-
on day 2 in solid state fermentations, decreases thereafter
0.25
-
acetylated xylan as substrate
0.37
-
purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl vanillate
0.39
-
p-coumaroyl
0.426
-
with 5 mM methyl p-coumarate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0
0.456
-
with 5 mM methyl ferulate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0
0.462
-
with feruloylated oligosaccharides as substrate, in 50 mM sodium phosphate buffer, pH 8.0
0.511
-
with 5 mM methyl sinapate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0
0.56
methyl sinapinate as substrate, pH 6.0, 37°C
0.57
methyl ferulate as substrate, pH 6.0, 37°C
0.598
-
11fold purified FAE-1
0.67
transesterification, pH 6.0, 45°C in 19.8: 74.7: 5.5 v/v/v n-hexane: t-butanol: 100 mM MOPSNaOH
0.7
-
triacetin as substrate
0.84
recombinant enzyme, substrate 4-nitrophenyl decanoate, pH 6.8, 40°C
1.1
-
purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl sinapinate
1.23
-
activity toward ferulic acid methyl ester
1.51
-
de-starched wheat bran as substrate
1.54
-
purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl caffeate
1.63
-
p-nitrophenyl butyrate
1.7
-
with 1 mM p-nitrophenyl myristate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0
1.85
purified enzyme with a yield of 19.54%
1.89
-
purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl vanillate
1.91
-
p-nitrophenyl butyrate
10.02
-
purified recombinant isozyme R43, pH 7.0, 50°C, substrate ethyl ferulate
10.2
recombinant enzyme, substrate methyl sinapate, pH 6.8, 40°C
10.3
-
methyl coumarate as substrate
10.4
purified recombinant enzyme, pH 8.0, 38°C
10.6
substrate methyl ferulate, pH 7.2, 25°C
10.9
-
purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl sinapinate
100
-
FAE activity decreases up to 24 h of culture and is maintained at 0.1 mU/mg protein with destarched wheat bran (1%) as the carbon source, at pH 6.5 and 40°C
1027
substrate methyl ferulate, pH 5.0, 37°C
12.4
-
above, purified recombinant enzyme, substrate is methyl 4-coumarate, pH 7.0, 40°C
12.7
-
o-nitrophenol acetate as substrate
12.9
-
methyl sinapinate as substrate, 30°C
13.14
-
substrate methyl caffeate, pH 4.0, 30°C
13.32
-
ammonium sulfate fraction, de-esterified corncobs as carbon source
13.75
-
purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl 4-coumarate
136.5
recombinant enzyme, substrate 4-nitrophenyl acetate, pH 6.8, 40°C
1397
-
substrate 5-O-trans-feruloyl-L-arabinofuranose, pH 5.0, 37°C
14.11
-
ammonium sulfate fraction, corncobs as carbon source
14.4
-
feruloyl/p-coumaroyl esterase, methyl ferulate as substrate
14.77
-
ammonium sulfate fraction, wheat germ as carbon source
15.61
-
ammonium sulfate fraction, de-starched wheat straw as carbon source
156
-
methyl sinapinate as substrate
16.6
purified recombinant enzyme, pH 7.0, 35°C
160
-
with methyl cinnamate as substrate, at pH 6.5 and 40°C
170
-
purified enzyme, pH 5.0, 37°C
174.4
substrate methyl p-coumarate, pH 6.5, 30°C
18.2
hydrolysis of vinyl ferulate, pH 6.0, 45°C
18.5
-
with 1 mM p-nitrophenyl acetate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0
18.97
-
purified recombinant isozyme R18, pH 7.0, 50°C, substrate ethyl ferulate
19.3
hydrolysis of vinyl ferulate, pH 6.0, 45°C
19.31
19.8
-
purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl ferulate
199
-
with ethyl 3-coumarincarboxylate as substrate, in 100 mM phosphate buffer, pH 7.2, at 37°C
2.19
-
extracted and filtrated fraction, de-starched corncobs as carbon source
2.191
2.24
-
extracted and filtrated fraction, de-starched wheat straw as carbon source
2.241
2.26
-
extracted and filtrated fraction, wheat germ as carbon source
2.262
2.29
substrate methyl 4-coumarate, pH 7.2, 25°C
2.31
-
extracted and filtrated fraction, de-esterified wheat straw as carbon source
2.319
2.32
-
extracted and filtrated fraction, corncobs as carbon source
2.321
2.357
2.36
-
extracted and filtrated fraction, de-esterified corncobs as carbon source
2.52
-
first DEAE-cellulose fraction, de-starched wheat straw as carbon source
2.56
-
first DEAE-cellulose fraction, wheat germ as carbon source
2.6
-
with ethyl cinnamate as substrate, in 100 mM phosphate buffer, pH 7.2, at 37°C
2.71
-
first DEAE-cellulose fraction, wheat straw as carbon source
2.82
-
first DEAE-cellulose fraction, corncobs as carbon source
2.89
-
purified enzyme, pH 7, 40°C
2.9
-
with xylan birchwood as substrate and p-coumaric acid as product, in 100 mM phosphate buffer, pH 7.2, at 37°C
20.3 - 25.4
substrate feruloyl arabino-xylo-oligosaccharides, recombinant His-tagged enzyme, pH 7.0, 37°C
21.6
-
methyl ferulate as substrate, 30°C
210
-
with oat spelt xylan (1%) as the carbon source, at pH 6.5 and 40°C
219.1
recombinant enzyme, substrate 4-nitrophenyl butyrate, pH 6.8, 40°C
22
substrate methyl ferulate, pH 3.0, 70°C
22.4
-
methyl ferulate as substrate
22.5
-
methyl ferulate as substrate
220
-
with destarched wheat bran (1%) as the carbon source or ethyl cinnamate as substrate, at pH 6.5 and 40°C
23.07
-
purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl ferulate
263.3
purified isozyme, pH 5.0, 40°C
275.5
purified isozyme, pH 5.0, 40°C
28
-
above, purified recombinant enzyme, substrate is methyl ferulate, pH 7.0, 40°C
28.4
-
ferulic acid methyl ester as substrate
29.5
wild-type enzyme, substrate 4-nitrophenyl ferulate, pH 6.4, 40°C
3
-
de-starched wheat bran as substrate in absence of xylanase
3.2
-
PAXX as substrate
3.33
-
first DEAE-cellulose fraction, de-esterified corncobs as carbon source
3.57
-
second DEAE-cellulose fraction, de-esterified wheat straw as carbon source
3.8
-
methyl ferulate as substrate, pH 7, 40°C
3.85
-
second DEAE-cellulose fraction, wheat straw as carbon source
30.4
-
methyl caffeate as substrate, 30°C
300
-
with methyl p-coumarate as substrate, at pH 6.5 and 40°C
31
-
de-starched wheat bran as substrate in presence of xylanase
31.8
-
p-nitrophenyl acetate as substrate, 30°C
317.7
purified isozyme, pH 5.0, 40°C
33.7
-
p-coumaric acid methyl ester
35.3
-
methyl p-coumarate as substrate, 30°C
35.9
-
O-{5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl}-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose as substrate, 30°C
36
-
with 1 mM p-nitrophenyl butyrate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0
36.1
-
methyl p-coumarate as substrate
37
-
with ethyl ferulate as substrate, in 100 mM phosphate buffer, pH 7.2, at 37°C
38.9
-
methyl 3,4-dimethoxycinnamate as substrate
4
-
with hemicellulose of ryegrass as substrate and trans-cinnamic acid as product, in 100 mM phosphate buffer, pH 7.2, at 37°C
4.1
-
with hemicellulose of ryegrass as substrate and p-coumaric acid as product, in 100 mM phosphate buffer, pH 7.2, at 37°C
4.15
-
first DEAE-cellulose fraction, de-starched corncobs as carbon source
4.8
transesterification, pH 6.0, 45°C in 19.8: 74.7: 5.5 v/v/v n-hexane: t-butanol: 100 mM MOPSNaOH
47.3
hydrolysis of L-arabinosyl ferulate, pH 6.0, 45°C
477.6
substrate methyl caffeate, pH 6.5, 30°C
5.25
-
pH 4.5, 45°C
5.3
-
2-O-5-O-(transferuloyl)-beta-L-arabinofuranosyl-D-xylopyranose as substrate
50
-
purifed recombinant enzyme, substrate is 4-nitrophenyl acetate, pH 7.0, 40°C
57.1
hydrolysis of L-arabinosyl ferulate, pH 6.0, 45°C
57.9
-
substrate methyl ferulate, pH 7.2, 30°C
58
-
methyl coumarate as substrate
6.75
-
purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl 4-coumarate
6.9
-
with hemicellulose of ryegrass as substrate and ferulic acid as product, in 100 mM phosphate buffer, pH 7.2, at 37°C
6.92
-
second DEAE-cellulose fraction, de-starched wheat straw as carbon source
6.94
-
purified enzyme, pH 6.0, 45°C
60
-
with destarched wheat bran (1%) as the carbon source and in the presence of 0.17 g/L ferulic acid, at pH 6.5 and 40°C
600
-
after 14 h of culture with destarched wheat bran (1%) as the carbon source, at pH 6.5 and 40°C
63
-
methyl sinapate as substrate
67.2
-
methyl ferulate as substrate
68.8
-
purified enzyme, 30°C
7
substrate methyl sinapinate, pH 7.2, 25°C
7.1
-
with xylan birchwood as substrate and 3-coumarincarboxylic acid as product, in 100 mM phosphate buffer, pH 7.2, at 37°C
7.3
recombinant enzyme, substrate methyl ferulate, pH 6.8, 40°C
7.9
-
with xylan birchwood as substrate and trans-cinnamic acid as product, in 100 mM phosphate buffer, pH 7.2, at 37°C
71.1
-
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside as substrate, 30°C
720
-
substrate methyl 3,4-dimethoxycinnamate, pH 6.0, 37°C
73.2
-
4-nitrophenyl 4-O-trans-feruloylxylopyranoside as substrate, 30°C
74.6
-
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside as substrate, 30°C
75
-
methyl ferulate as substrate
8.06
-
methyl caffeate as substrate
8.28
-
second DEAE-cellulose fraction, corncobs as carbon source
8.99
-
second DEAE-cellulose fraction, de-starched corncobs as carbon source
800
-
3.6fold purified enzyme
84.2
-
methyl p-coumarate as substrate
88
-
ethyl ferulate as substrate
9.1
recombinant enzyme, substrate 4-nitrophenyl octanoate, pH 6.8, 40°C
9.63
substrate 4-nitrophenyl ferulate, pH 6.0, 50°C
9.66
-
substrate methyl ferulate, pH 4.0, 30°C
9.82
-
second DEAE-cellulose fraction, de-esterified corncobs as carbon source
96.9
-
methyl caffeate as substrate
additional information