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Literature summary for 3.1.1.73 extracted from

  • Dzedzyulya, E.I.; Becker, E.G.; Okunev, O.N.; Sinitsyn, A.P.
    Feruloyl esterase from Aspergillus sp.: Purification, properties, and action on natural substrates (1999), Biochemistry (Moscow), 64, 405-410.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus sp.
-
-
extracellular
-
Aspergillus awamori
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
-
Aspergillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl-polysaccharide + H2O Aspergillus niger
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Aspergillus sp.
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Aspergillus awamori
-
ferulate + polysaccharide
-
?
sugar beet pulp + H2O Aspergillus niger
-
ferulic acid + ?
-
?
sugar beet pulp + H2O Aspergillus sp.
-
ferulic acid + ?
-
?
wheat bran + H2O Aspergillus niger
-
ferulic acid + p-coumaric acid + ?
-
?
wheat bran + H2O Aspergillus sp.
-
ferulic acid + p-coumaric acid + ?
-
?
wheat bran + H2O Aspergillus awamori
-
ferulic acid + p-coumaric acid + ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
-
-
Aspergillus niger
-
-
-
Aspergillus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.23
-
activity toward ferulic acid methyl ester Aspergillus sp.
26
-
activity toward feruloyl arabinoside Aspergillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
Aspergillus sp. acetic acid + 1-naphthol
-
?
1-naphthyl acetate + H2O
-
Aspergillus awamori acetic acid + 1-naphthol
-
?
ferulic acid methyl ester + H2O
-
Aspergillus niger ferulic acid + methanol
-
?
ferulic acid methyl ester + H2O
-
Aspergillus sp. ferulic acid + methanol
-
?
ferulic acid methyl ester + H2O
-
Aspergillus awamori ferulic acid + methanol
-
?
feruloyl arabinoside + H2O structural analog of natural substrates of feruloyl esterases Aspergillus sp. ferulic acid + coumaric acid + cinnamic acid + arabinose
-
?
feruloyl-polysaccharide + H2O
-
Aspergillus niger ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Aspergillus sp. ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Aspergillus awamori ferulate + polysaccharide
-
?
additional information
-
Aspergillus niger ?
-
?
additional information
-
Aspergillus awamori ?
-
?
additional information does not hydrolyze wheat straw, purified enzyme is inactive towards the p-coumaric methyl ester Aspergillus sp. ?
-
?
p-coumaric acid methyl ester + H2O
-
Aspergillus niger p-coumaric acid + methanol
-
?
p-coumaric acid methyl ester + H2O
-
Aspergillus sp. p-coumaric acid + methanol
-
?
p-coumaric acid methyl ester + H2O
-
Aspergillus awamori p-coumaric acid + methanol
-
?
p-nitrophenyl acetate + H2O
-
Aspergillus niger p-nitrophenol + acetate
-
?
p-nitrophenyl acetate + H2O
-
Aspergillus sp. p-nitrophenol + acetate
-
?
p-nitrophenyl acetate + H2O
-
Aspergillus awamori p-nitrophenol + acetate
-
?
sugar beet pulp + H2O
-
Aspergillus niger ferulic acid + ?
-
?
sugar beet pulp + H2O
-
Aspergillus sp. ferulic acid + ?
-
?
wheat bran + H2O
-
Aspergillus niger ferulic acid + p-coumaric acid + ?
-
?
wheat bran + H2O
-
Aspergillus sp. ferulic acid + p-coumaric acid + ?
-
?
wheat bran + H2O
-
Aspergillus awamori ferulic acid + p-coumaric acid + ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
maximal activity toward ferulic acid methyl ester Aspergillus sp.
50 60 maximal activity toward feruloyl arabinoside Aspergillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
-
Aspergillus awamori
50
-
completely inactivated within 30 min at 70°C Aspergillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 6
-
Aspergillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
-
Aspergillus sp.