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Literature summary for 3.1.1.73 extracted from

  • Romero-Borbon, E.; Grajales-Hernandez, D.; Armendariz-Ruiz, M.; Ramirez-Velasco, L.; Rodriguez-Gonzalez, J.; Cira-Chavez, L.; Estrada-Alvarado, M.; Mateos-Diaz, J.
    Type C feruloyl esterase from Aspergillus ochraceus A butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system (2018), Electron. J. Biotechnol., 35, 1-9 .
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 83% of initial activity Aspergillus ochraceus
Cu2+ 1 mM, 55% of initial activity Aspergillus ochraceus
Hg2+ 1 mM, 10% of initial activity Aspergillus ochraceus
PMSF 1 mM, no residual activity Aspergillus ochraceus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
methyl caffeate pH 7.2, 30°C Aspergillus ochraceus
0.58
-
methyl p-coumarate pH 7.2, 30°C Aspergillus ochraceus
0.64
-
methyl ferulate pH 7.2, 30°C Aspergillus ochraceus
1.11
-
methyl sinapinate pH 7.2, 30°C Aspergillus ochraceus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 209% of initial activity Aspergillus ochraceus
Mg2+ 1 mM, 177% of initial activity Aspergillus ochraceus
Mn2+ 1 mM, 143% of initial activity Aspergillus ochraceus
Zn2+ 1 mM, 112% of initial activity Aspergillus ochraceus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
gel filtration Aspergillus ochraceus

Organic Solvent Stability

Organic Solvent Comment Organism
Butanol maximum initial synthetic rate of 2.8 nmol/min for transesterification reaction to yiled butyl ferulate is reached at 25% (v/v) butanol, using an isooctane:butanol:water relation of 73:25:2 Aspergillus ochraceus

Organism

Organism UniProt Comment Textmining
Aspergillus ochraceus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus ochraceus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
57.9
-
substrate methyl ferulate, pH 7.2, 30°C Aspergillus ochraceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl caffeate + H2O
-
Aspergillus ochraceus methanol + caffeate
-
?
methyl ferulate + butanol
-
Aspergillus ochraceus methanol + butyl ferulate
-
?
methyl ferulate + H2O
-
Aspergillus ochraceus methanol + ferulate
-
?
methyl p-coumarate + H2O
-
Aspergillus ochraceus methanol + p-coumarate
-
?
methyl sinapinate + H2O
-
Aspergillus ochraceus methanol + sinapinate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 34600, SDS-PAGE Aspergillus ochraceus

Synonyms

Synonyms Comment Organism
FaeC
-
Aspergillus ochraceus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Aspergillus ochraceus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2306
-
methyl caffeate pH 7.2, 30°C Aspergillus ochraceus
2306
-
methyl sinapinate pH 7.2, 30°C Aspergillus ochraceus
2710
-
methyl p-coumarate pH 7.2, 30°C Aspergillus ochraceus
3344
-
methyl ferulate pH 7.2, 30°C Aspergillus ochraceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Aspergillus ochraceus

pH Range

pH Minimum pH Maximum Comment Organism
10
-
40% of maximum activity Aspergillus ochraceus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2078
-
methyl sinapinate pH 7.2, 30°C Aspergillus ochraceus
4352
-
methyl caffeate pH 7.2, 30°C Aspergillus ochraceus
4673
-
methyl p-coumarate pH 7.2, 30°C Aspergillus ochraceus
5226
-
methyl ferulate pH 7.2, 30°C Aspergillus ochraceus