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3.1.1.73: feruloyl esterase

This is an abbreviated version!
For detailed information about feruloyl esterase, go to the full flat file.

Word Map on EC 3.1.1.73

Reaction

feruloyl-polysaccharide
+
H2O
=
ferulate
 +
Polysaccharide

Synonyms

4-hydroxy-3-methoxycinnamic acid esterase, A.O.1, A.O.11, A.O.12, A.O.13, A.O.2, A.O.3, A.O.4, A.O.5, A.O.6, A.O.7, A.O.8, A.O.9, acetyl/ferulic acid esterase, AfFaeA, AN1772.2, AnFAE, AnFaeA, AnFAEB, AO090701000884, AoFaeB, AoFaeC, AtFAE-1, AtFAE-2, AtFAE-3, AuFaeA, AusFaeA, AwFAE, AwFAEA, BioH, carboxylic ester hydrolase, CE1, cellulosome multi-enzyme complex, cellulosome xylanase Z feruloyl esterase, CinI, CinII, cinnAE, cinnamic acid esterase, cinnamic acid hydrolase, cinnamic acid hydrolases, cinnamoyl ester hydrolase, Cinnamoyl esterase, CjXYLD, Est1, Est1E, EstA, esterase A, EstF27, FA esterase, FAE, FAE B, Fae-1, FAE-2, FAE-A, FAE-B, FAE-C, FAE-D, FAE-I, FAE-II, FAE-III, FAE-PL, FAE1, FAE125, Fae1A, FAE2, FAE3, FAE4, FAE5, FAE6, FAE68, FAE7, FAE7262, FAEA, FAEA1, FAEA2, FAEB, FaeB1, FAEB2, FaeC, FaeD, FaeD-3.544, FaeD1, FaeD2, FaeI, FaeT, FAE_XynZ, FE, FeE, Fee1B, ferulic acid esterase, ferulic acid esterase A, ferulic acid esterase B, ferulic acid esterase C, ferulic acid esterase D, feruloyl esterase, feruloyl esterase A, feruloyl esterase B, feruloyl/p-coumaroyl esterase, Feruloylesterase, FoFAE, FoFAE-I, FoFAE-II, FoFaeA, FoFaeB, FoFaeI, FoFaeII, hemicellulase acessory enzymes, hydroxycinnamoyl esterase, KX091144, Lp_0796, More, NcFaeB, NcFaeD-3.544, PeFaeA, PfFaeB, phenolic acid esterase, PSHAa enzyme, PSHAa1385, R18, SCHCODRAFT_60993, SCHCODRAFT_61055, StFAE, StFAE-A, StFaeB, StFaeC, TH2-18, TsFaeA, TsFaeB, TsFaeC, Tvms10a, Tvmz2a, type A FAE, type A feruloyl esterase, type B FAE, type B ferulic acid esterase, XLYD, XM 001217492, xylan-degrading enzyme system, xylanase 10, xylanase 10B, xylanase Z, xylanase-ferulic acid esterase, XYLD, XYLD esterase, Xyn10B, Xyn10D-Fae1A, XynZ

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.73 feruloyl esterase

Storage Stability

Storage Stability on EC 3.1.1.73 - feruloyl esterase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 4 months
-
-20°C, approximately 70% original activity retained after storage for 3 months
-
-20°C, approximately 78% activity retained after storage for 2 months
-
-20°C, pH 7, enzyme loses activity over 24 h even in presence of EDTA, DTT, Mg2+ and Ca2+, additives which stabilizes the enzyme during purification
-
25°C, storage for 150 days, the activity of mutant T27I/S84L/V161I/H195L/G243C/A259V remains almost constant (100.5%), whereas that of the wild-type is only 21.2%
4°C, crude culture filtrate, 6 months, 20% loss of activity
-
4°C, partially purified enzyme, 1 month, 50% loss of activity
-
4°C, pH 7, enzyme loses activity over 24 h even in presence of EDTA, DTT, Mg2+ and Ca2+, additives which stabilizes the enzyme during purification
-
4°C, stable for at least 2 months after hydrophobic interaction chromatography
-
activity remains above the minimum analysis specification for at least 6 months from the date of Batch Certificate of Analysis, when stored below 20°C
-
at room temperature, after 2 days feruloyl esterases enzyme loses about 15% of its initial activity and only about one third of the initial activity remains at the end of 1 week, on refrigeration, no apparent loss of activity is found up to 4 weeks, but after 4 weeks a marginal decrease in activity (5-10%) is found. The enzyme retains almost its full activity when stored deep frozen (-20°C) for up to 4 months.
-