Application | Comment | Organism |
---|---|---|
nutrition | broad range of application in the agri-food industries | Neurospora crassa |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris GS115 (his4) | Neurospora crassa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Neurospora crassa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28598 | - |
x * 28598, calculated from the deduced amino acid sequence | Neurospora crassa |
32000 | - |
x * 32000, SDS-PAGE | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl polysaccharide + H2O | Neurospora crassa | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + p-coumaric acid + ? | - |
? | |
spent grain + H2O | Neurospora crassa | incubation together with xylanase | ferulic acid + p-coumaric acid + ? | low percentage of p-coumaric acid | ? | |
wheat bran + H2O | Neurospora crassa | incubation together with xylanase | ferulic acid + p-coumaric acid + ? | monomeric and dehydrodimers of ferulic acid | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | Q9HGR3 | type D sub-class of the enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | possible glycosylation hypothesized from the mass difference between the theoretical mass and the SDS-PAGE results | Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
methyl caffeate as substrate, pH 6.0, 37°C | Neurospora crassa |
0.3 | - |
methyl p-coumarate as substrate, pH 6.0, 37°C | Neurospora crassa |
0.56 | - |
methyl sinapinate as substrate, pH 6.0, 37°C | Neurospora crassa |
0.57 | - |
methyl ferulate as substrate, pH 6.0, 37°C | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Neurospora crassa | ferulic acid + p-coumaric acid + ? | - |
? | |
methyl caffeate + H2O | - |
Neurospora crassa | caffeic acid + methanol | - |
? | |
methyl ferulate + H2O | - |
Neurospora crassa | ferulic acid + methanol | - |
? | |
methyl p-coumarate + H2O | - |
Neurospora crassa | p-coumaric acid + methanol | - |
? | |
methyl sinapinate + H2O | - |
Neurospora crassa | sinapic acid + methanol | - |
? | |
spent grain + H2O | incubation together with xylanase | Neurospora crassa | ferulic acid + p-coumaric acid + ? | low percentage of p-coumaric acid | ? | |
wheat bran + H2O | incubation together with xylanase | Neurospora crassa | ferulic acid + p-coumaric acid + ? | monomeric and dehydrodimers of ferulic acid | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Neurospora crassa |
? | x * 28598, calculated from the deduced amino acid sequence | Neurospora crassa |