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Literature summary for 3.1.1.73 extracted from
- Xylan-degrading enzyme system produced by the fungus Aspergillus awamori: isolation and characterization of a feruloyl esterase and a p-coumaroyl esterase (1994), Enzyme Microb. Technol., 16, 826-834.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Aspergillus awamori |  |
| Ba2+ | inhibits feruloyl esterase, no effect on p-coumaroyl esterase | Aspergillus awamori | |
| Fe2+ | - |
Aspergillus awamori | |
| Fe2+ | - |
Talaromyces pinophilus | |
| Hg2+ | - |
Aspergillus awamori | |
| Zn2+ | inhibits feruloyl esterase, no effect on p-coumaroyl esterase | Aspergillus awamori | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
methyl p-coumarate | - |
Aspergillus awamori | |
| 0.14 | - |
ferulic acid methyl ester | - |
Talaromyces pinophilus | |
| 0.93 | - |
methyl ferulate | - |
Aspergillus awamori | |
| 1.86 | - |
methyl ferulate | - |
Streptomyces olivochromogenes | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces olivochromogenes | - |
- |
| extracellular | - |
Aspergillus awamori | - |
- |
| extracellular | - |
Talaromyces pinophilus | - |
- |
| extracellular | - |
Neocallimastix sp. | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11000 | - |
p-coumaroyl esterase | Neocallimastix sp. |
| 24000 | - |
- |
Neocallimastix sp. |
| 29000 | - |
- |
Streptomyces olivochromogenes |
| 30000 | - |
- |
Aspergillus oryzae |
| 57000 | - |
- |
Talaromyces pinophilus |
| 69000 | - |
- |
Neocallimastix sp. |
| 75000 | - |
p-coumaroyl esterase, SDS-PAGE | Aspergillus awamori |
| 112000 | - |
ferruloyl esterase, SDS-PAGE | Aspergillus awamori |
| 132000 | - |
- |
Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl xylooligosaccharide + H2O | Streptomyces olivochromogenes | - |
ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | Aspergillus oryzae | - |
ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | Aspergillus phoenicis | - |
ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | Fibrobacter succinogenes | - |
ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | Aspergillus awamori | - |
ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | Talaromyces pinophilus | - |
ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | Neocallimastix sp. | - |
ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Aspergillus niger | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Streptomyces olivochromogenes | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Aspergillus oryzae | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Aspergillus phoenicis | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Fibrobacter succinogenes | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Schizophyllum commune | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Aspergillus awamori | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Streptomyces viridosporus | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Talaromyces pinophilus | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Neocallimastix sp. | - |
ferulate + polysaccharide | - |
? | |
| italian rye gras cell walls + H2O | Aspergillus awamori | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
| italian rye gras cell walls + H2O | Talaromyces pinophilus | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
| maize internodes + H2O | Aspergillus awamori | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
| oat straw + H2O | Aspergillus awamori | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
| wheat bran + H2O | Aspergillus niger | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
| wheat bran + H2O | Aspergillus awamori | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus awamori | - |
- |
- |
| Aspergillus awamori IMI 142717 | - |
- |
- |
| Aspergillus niger | - |
- |
- |
| Aspergillus oryzae | - |
- |
- |
| Aspergillus phoenicis | - |
- |
- |
| Fibrobacter succinogenes | - |
- |
- |
| Neocallimastix sp. | - |
- |
- |
| Schizophyllum commune | - |
- |
- |
| Streptomyces olivochromogenes | - |
- |
- |
| Streptomyces viridosporus | - |
- |
- |
| Talaromyces pinophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus awamori |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.39 | - |
p-coumaroyl | Aspergillus awamori |
| 2.5 | - |
feruoyl esterase | Aspergillus awamori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus awamori | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus awamori IMI 142717 | 4-nitrophenol + D-glucopyranose | - |
? | |
| acetylxylan + H2O | - |
Aspergillus awamori | acetic acid + ? | - |
? | |
| acetylxylan + H2O | - |
Talaromyces pinophilus | acetic acid + ? | - |
? | |
| acetylxylan + H2O | - |
Aspergillus awamori IMI 142717 | acetic acid + ? | - |
? | |
| ferulic acid methyl ester + H2O | - |
Aspergillus niger | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Streptomyces olivochromogenes | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Aspergillus awamori | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Talaromyces pinophilus | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Neocallimastix sp. | ferulic acid + methanol | - |
? | |
| feruloyl xylooligosaccharide + H2O | - |
Streptomyces olivochromogenes | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | - |
Aspergillus oryzae | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | - |
Aspergillus phoenicis | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | - |
Fibrobacter succinogenes | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | - |
Aspergillus awamori | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | - |
Talaromyces pinophilus | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | - |
Neocallimastix sp. | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus niger | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Streptomyces olivochromogenes | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus oryzae | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus phoenicis | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Fibrobacter succinogenes | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Schizophyllum commune | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus awamori | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Streptomyces viridosporus | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Talaromyces pinophilus | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Neocallimastix sp. | ferulate + polysaccharide | - |
? | |
| italian rye gras cell walls + H2O | - |
Aspergillus awamori | ferulic acid + p-coumaric acid + ? | - |
? | |
| italian rye gras cell walls + H2O | - |
Talaromyces pinophilus | ferulic acid + p-coumaric acid + ? | - |
? | |
| maize internodes + H2O | - |
Aspergillus awamori | ferulic acid + p-coumaric acid + ? | - |
? | |
| additional information | - |
Aspergillus niger | ? | - |
? | |
| additional information | - |
Aspergillus awamori | ? | - |
? | |
| additional information | - |
Talaromyces pinophilus | ? | - |
? | |
| additional information | - |
Neocallimastix sp. | ? | - |
? | |
| additional information | unable to attack feruloylated xylan in bran | Streptomyces olivochromogenes | ? | - |
? | |
| additional information | - |
Aspergillus awamori IMI 142717 | ? | - |
? | |
| O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Aspergillus awamori | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Neocallimastix sp. | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| oat straw + H2O | - |
Aspergillus awamori | ferulic acid + p-coumaric acid + ? | - |
? | |
| p-coumaric acid methyl ester + H2O | - |
Aspergillus niger | p-coumaric acid + methanol | - |
? | |
| p-coumaric acid methyl ester + H2O | - |
Streptomyces olivochromogenes | p-coumaric acid + methanol | - |
? | |
| p-coumaric acid methyl ester + H2O | - |
Aspergillus awamori | p-coumaric acid + methanol | - |
? | |
| p-coumaric acid methyl ester + H2O | - |
Talaromyces pinophilus | p-coumaric acid + methanol | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Aspergillus niger | p-nitrophenol + acetate | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Aspergillus awamori | p-nitrophenol + acetate | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Talaromyces pinophilus | p-nitrophenol + acetate | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Aspergillus awamori IMI 142717 | p-nitrophenol + acetate | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus awamori | p-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| wheat bran + H2O | - |
Aspergillus niger | ferulic acid + p-coumaric acid + ? | - |
? | |
| wheat bran + H2O | - |
Aspergillus awamori | ferulic acid + p-coumaric acid + ? | - |
? | |
| xylan polysaccharide + H2O | - |
Aspergillus niger | ferulic acid + p-coumaric acid | - |
? | |
| xylan polysaccharide + H2O | - |
Aspergillus oryzae | ferulic acid + p-coumaric acid | - |
? | |
| xylan polysaccharide + H2O | - |
Aspergillus awamori | ferulic acid + p-coumaric acid | - |
? | |
| xylan polysaccharide + H2O | - |
Talaromyces pinophilus | ferulic acid + p-coumaric acid | - |
? | |
| xylan polysaccharide + H2O | - |
Aspergillus awamori IMI 142717 | ferulic acid + p-coumaric acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xylan-degrading enzyme system | - |
Aspergillus awamori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus awamori |
| 50 | - |
- |
Neocallimastix sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | - |
Aspergillus awamori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | loses most of its activity when heated for 10 min at 70°C | Aspergillus awamori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus niger |
| 5 | - |
both feruloyl esterase and p-coumaroyl esterase | Aspergillus awamori |
| 5.5 | - |
- |
Streptomyces olivochromogenes |
| 5.5 | - |
p-coumaroyl esterase | Streptomyces viridosporus |
| 6 | - |
- |
Aspergillus niger |
| 6 | - |
- |
Talaromyces pinophilus |
| 6.2 | 7 | feruloyl esterase | Neocallimastix sp. |
| 7.5 | - |
p-coumaroyl esterase | Neocallimastix sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8.1 | - |
Aspergillus awamori |
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