KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
methyl caffeate | - |
Thermothelomyces heterothallicus | |
0.57 | - |
methyl sinapinate | - |
Thermothelomyces heterothallicus | |
0.57 | - |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Thermothelomyces heterothallicus | |
0.59 | - |
methyl p-coumarate | - |
Thermothelomyces heterothallicus | |
0.62 | - |
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Thermothelomyces heterothallicus | |
1.64 | - |
methyl ferulate | - |
Thermothelomyces heterothallicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermothelomyces heterothallicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 23000, SDS-PAGE, native mass by native PAGE | Thermothelomyces heterothallicus |
43000 | - |
native PAGE | Thermothelomyces heterothallicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl polysaccharide + H2O | Thermothelomyces heterothallicus | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
wheat bran + H2O | Thermothelomyces heterothallicus | - |
ferulic acid + ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | activity is retained in hexane/1-butanol/water surfactantless microemulsion | Thermothelomyces heterothallicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces heterothallicus | - |
strain ATCC 34628, feruloyl esterase type C | - |
Purification (Comment) | Organism |
---|---|
- |
Thermothelomyces heterothallicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.1 | - |
purified enzyme | Thermothelomyces heterothallicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O | C-5 linked ferulate is hydrolyzed three times more efficiently than C-2 linked ferulate | Thermothelomyces heterothallicus | ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside | - |
? | |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O | C-5 linked ferulate is hydrolyzed three times more efficiently than C-2 linked ferulate | Thermothelomyces heterothallicus | ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside | - |
? | |
feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Thermothelomyces heterothallicus | ferulic acid + ? | - |
? | |
methyl caffeate + H2O | - |
Thermothelomyces heterothallicus | caffeic acid + methanol | - |
? | |
methyl ferulate + H2O | - |
Thermothelomyces heterothallicus | ferulic acid + methanol | - |
? | |
methyl p-coumarate + H2O | - |
Thermothelomyces heterothallicus | p-coumaric acid + methanol | - |
? | |
methyl sinapinate + H2O | - |
Thermothelomyces heterothallicus | sinapic acid + methanol | - |
? | |
wheat bran + H2O | - |
Thermothelomyces heterothallicus | ferulic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23000, SDS-PAGE, native mass by native PAGE | Thermothelomyces heterothallicus |
Synonyms | Comment | Organism |
---|---|---|
StFaeC | - |
Thermothelomyces heterothallicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Thermothelomyces heterothallicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
82% of activity | Thermothelomyces heterothallicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
methyl caffeate | - |
Thermothelomyces heterothallicus | |
0.4 | - |
methyl sinapinate | - |
Thermothelomyces heterothallicus | |
0.5 | - |
methyl p-coumarate | - |
Thermothelomyces heterothallicus | |
2.6 | - |
methyl ferulate | - |
Thermothelomyces heterothallicus | |
3.7 | - |
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Thermothelomyces heterothallicus | |
10.3 | - |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Thermothelomyces heterothallicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermothelomyces heterothallicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | - |
80% of activity | Thermothelomyces heterothallicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | stable, 80% of activity after 24 h at pH 10 | Thermothelomyces heterothallicus |
10 | - |
half life at 55°C: 133 min, half life at 60°C: 55 min | Thermothelomyces heterothallicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermothelomyces heterothallicus | isoelectric focusing and cation-exchange chromatofocusing | - |
3.1 |