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Literature summary for 3.1.1.73 extracted from
- Purification and characterization of a Fusarium oxysporum feruloyl esterase (FoFAE-I) catalysing transesterification of phenolic acid esters (2003), Enzyme Microb. Technol., 33, 729-737.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | ferulic, p-coumaric, caffeic, and sinapinic acid have widespread industrial potential due to their antioxidant properties | Fusarium oxysporum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside | pH 6.0, 45°C | Fusarium oxysporum |  |
| 0.2 | - |
methyl p-coumarate | pH 6.0, 45°C | Fusarium oxysporum | |
| 0.26 | - |
methyl caffeate | pH 6.0, 45°C | Fusarium oxysporum | |
| 0.6 | - |
methyl ferulate | pH 6.0, 45°C | Fusarium oxysporum | |
| 1.12 | - |
methyl sinapinate | pH 6.0, 45°C | Fusarium oxysporum | |
| 1.6 | - |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside | pH 6.0, 45°C | Fusarium oxysporum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Fusarium oxysporum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
gel filtration | Fusarium oxysporum |
| 31000 | - |
1 * 31000, SDS-PAGE | Fusarium oxysporum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| destarched wheat bran + H2O | Fusarium oxysporum | most effective in the presence of added xylanase | ferulic acid + ? | - |
? | |
| destarched wheat bran + H2O | Fusarium oxysporum F3 | most effective in the presence of added xylanase | ferulic acid + ? | - |
? | |
| plant cell wall + H2O | Fusarium oxysporum | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
| plant cell wall + H2O | Fusarium oxysporum F3 | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Butanol | transesterifiaction in microemulsion of hexane/1-butanol/water | Fusarium oxysporum |
| hexane | transesterifiaction in microemulsion of hexane/1-butanol/water | Fusarium oxysporum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium oxysporum | - |
- |
- |
| Fusarium oxysporum F3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fusarium oxysporum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.94 | - |
purified enzyme, pH 6.0, 45°C | Fusarium oxysporum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O | C-2 position is hydrolyzed faster and more effectively than C-5 position | Fusarium oxysporum | ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside | - |
? | |
| 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O | C-2 position is hydrolyzed faster and more effectively than C-5 position | Fusarium oxysporum F3 | ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside | - |
? | |
| 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O | C-2 position is hydrolyzed faster and more effectively than C-5 position | Fusarium oxysporum | ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside | - |
? | |
| destarched wheat bran + H2O | most effective in the presence of added xylanase | Fusarium oxysporum | ferulic acid + ? | - |
? | |
| destarched wheat bran + H2O | most effective in the presence of added xylanase | Fusarium oxysporum F3 | ferulic acid + ? | - |
? | |
| methyl caffeate + 1-butanol | transesterifiaction in microemulsion of hexane/1-butanol/water | Fusarium oxysporum | methanol + butyl caffeate | - |
? | |
| methyl caffeate + H2O | - |
Fusarium oxysporum | caffeic acid + methanol | - |
? | |
| methyl caffeate + H2O | - |
Fusarium oxysporum F3 | caffeic acid + methanol | - |
? | |
| methyl ferulate + 1-butanol | transesterifiaction in microemulsion of hexane/1-butanol/water | Fusarium oxysporum | methanol + butyl ferulate | - |
? | |
| methyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + methanol | - |
? | |
| methyl p-coumarate + 1-butanol | transesterifiaction in microemulsion of hexane/1-butanol/water | Fusarium oxysporum | methanol + butyl p-coumarate | - |
? | |
| methyl p-coumarate + H2O | most effective substrate | Fusarium oxysporum | p-coumaric acid + methanol | - |
? | |
| methyl sinapinate + 1-butanol | transesterifiaction in microemulsion of hexane/1-butanol/water | Fusarium oxysporum | methanol + butyl sinapinate | - |
? | |
| methyl sinapinate + H2O | poor substrate | Fusarium oxysporum | sinapic acid + methanol | - |
? | |
| methyl sinapinate + H2O | poor substrate | Fusarium oxysporum F3 | sinapic acid + methanol | - |
? | |
| plant cell wall + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Fusarium oxysporum | ferulic acid + ? | - |
? | |
| plant cell wall + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Fusarium oxysporum F3 | ferulic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31000, SDS-PAGE | Fusarium oxysporum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FoFAE-I | - |
Fusarium oxysporum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Fusarium oxysporum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
fully stable at pH 7.0-9.0 | Fusarium oxysporum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
methyl sinapinate | pH 6.0, 45°C | Fusarium oxysporum | |
| 5.9 | - |
methyl caffeate | pH 6.0, 45°C | Fusarium oxysporum | |
| 6.9 | - |
methyl ferulate | pH 6.0, 45°C | Fusarium oxysporum | |
| 19.9 | - |
methyl p-coumarate | pH 6.0, 45°C | Fusarium oxysporum | |
| 166.7 | - |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside | pH 6.0, 45°C | Fusarium oxysporum | |
| 600 | - |
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside | pH 6.0, 45°C | Fusarium oxysporum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Fusarium oxysporum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | fully stable at 30°C | Fusarium oxysporum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Fusarium oxysporum | isoelectric focusing | - |
9.5 |
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