3.1.1.73: feruloyl esterase This is an abbreviated version! For detailed information about feruloyl esterase, go to the full flat file .
Reaction
feruloyl-polysaccharide +
H2O =
ferulate +
Polysaccharide
Synonyms 4-hydroxy-3-methoxycinnamic acid esterase, A.O.1, A.O.11, A.O.12, A.O.13, A.O.2, A.O.3, A.O.4, A.O.5, A.O.6, A.O.7, A.O.8, A.O.9, acetyl/ferulic acid esterase, AfFaeA, AN1772.2, AnFAE, AnFaeA, AnFAEB, AO090701000884, AoFaeB, AoFaeC, AtFAE-1, AtFAE-2, AtFAE-3, AuFaeA, AusFaeA, AwFAE, AwFAEA, BioH, carboxylic ester hydrolase, CE1, cellulosome multi-enzyme complex, cellulosome xylanase Z feruloyl esterase, CinI, CinII, cinnAE, cinnamic acid esterase, cinnamic acid hydrolase, cinnamic acid hydrolases, cinnamoyl ester hydrolase, Cinnamoyl esterase, CjXYLD, Est1, Est1E, EstA, esterase A, EstF27, FA esterase, FAE, FAE B, Fae-1, FAE-2, FAE-A, FAE-B, FAE-C, FAE-D, FAE-I, FAE-II, FAE-III, FAE-PL, FAE1, FAE125, Fae1A, FAE2, FAE3, FAE4, FAE5, FAE6, FAE68, FAE7, FAE7262, FAEA, FAEA1, FAEA2, FAEB, FaeB1, FAEB2, FaeC, FaeD, FaeD-3.544, FaeD1, FaeD2, FaeI, FaeT, FAE_XynZ, FE, FeE, Fee1B, ferulic acid esterase, ferulic acid esterase A, ferulic acid esterase B, ferulic acid esterase C, ferulic acid esterase D, feruloyl esterase, feruloyl esterase A, feruloyl esterase B, feruloyl/p-coumaroyl esterase, Feruloylesterase, FoFAE, FoFAE-I, FoFAE-II, FoFaeA, FoFaeB, FoFaeI, FoFaeII, hemicellulase acessory enzymes, hydroxycinnamoyl esterase, KX091144, Lp_0796, More, NcFaeB, NcFaeD-3.544, PeFaeA, PfFaeB, phenolic acid esterase, PSHAa enzyme, PSHAa1385, R18, SCHCODRAFT_60993, SCHCODRAFT_61055, StFAE, StFAE-A, StFaeB, StFaeC, TH2-18, TsFaeA, TsFaeB, TsFaeC, Tvms10a, Tvmz2a, type A FAE, type A feruloyl esterase, type B FAE, type B ferulic acid esterase, XLYD, XM 001217492, xylan-degrading enzyme system, xylanase 10, xylanase 10B, xylanase Z, xylanase-ferulic acid esterase, XYLD, XYLD esterase, Xyn10B, Xyn10D-Fae1A, XynZ
ECTree
Molecular Weight
Molecular Weight on EC 3.1.1.73 - feruloyl esterase
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101000
-
recombinant deglycosylated enzyme, gel filtration
112000
-
ferruloyl esterase, SDS-PAGE
14500
-
gel filtration, anomalous retardation of the purified enzyme
145000
-
CinnAE, gel filtration
18000
-
gel filtration, anomalous retardation of the purified enzyme
18600
x * 18600, recombinant His6-tagged enzyme, SDS-PAGE
2000000
-
cellulosome enzyme-complex
28164
-
x * 40000, recombinant enzyme, SDS-PAGE, x * 28164, sequence calculation
28300
-
x * 36000, recombinant enzyme, SDS-PAGE, x * 28300, about, sequence calculation
28550
FAEA produced in Escherichia coli, mass spectrometry
28598
x * 28598, calculated from the deduced amino acid sequence
29100
-
sequence analysis
29286
x * 29286, calculated from the deduced amino acid sequence, x * 35000, SDS-PAGE, x * 35040, average mass from mass spertrometry
30250
FAEA produced in Aspergillus niger, mass spectrometry
31200
x * 31200, recombinant His6-tagged enzyme, SDS-PAGE
35040
x * 29286, calculated from the deduced amino acid sequence, x * 35000, SDS-PAGE, x * 35040, average mass from mass spertrometry
35450
-
predicted molecular mass of mature polypeptide
38000
-
x * 38000, extracellular isozyme R18, SDS-PAGE, x * 52000, extracellular isozyme R43, SDS-PAGE
52000
-
x * 38000, extracellular isozyme R18, SDS-PAGE, x * 52000, extracellular isozyme R43, SDS-PAGE
56107
-
x * 56107, calculated from sequence
59200
1 * 67000, SDS-PAGE, 1 * 59200, about, sequence calculation
68000
-
FAE-I, high-performance gel filtration
72000
Q70Y21
1 * 55340, calculated from the deduced amino acid sequence, 1 * 72000, SDS-PAGE
78000
-
size-exclusion chromatography
additional information
-
a molecular weight of 12400 Da estimated by gel filtration, the difference between the molecular weights obtained using SDS or native PAGE and gel filtration might be caused by an affinity of the enzyme to the silica gel particles used for the chromatography
11000
-
-
11000
-
p-coumaroyl esterase
132000
-
-
210000
-
-
23000
-
23000
x * 23000, SDS-PAGE
23000
-
2 * 23000, SDS-PAGE, native mass by native PAGE
24000
-
-
24000
-
FAE-II, denaturing and nondenaturing PAGE, gel filtration, high performance gel filtration
27000
-
27000
-
isoenzyme FoFAE-II
29000
-
-
29740
-
FAEA, mass spectrometry
29740
-
FAE-III, mass spectrometry
30000
-
-
31000
-
-
31000
-
isoenzyme FoFAE-I
31000
-
x * 31000, SDS-PAGE
31000
-
1 * 31000, SDS-PAGE
32000
isoenzyme NcFaeD-3.544
32000
x * 32000, SDS-PAGE
33000
-
-
33000
-
SDS-PAGE, gel filtration
33000
-
isoenzyme StFAE-A
33000
-
1 * 33000, SDS-PAGE, purified enzyme
35000
Q70Y21
-
35000
Q70Y21
isoenzyme TsFaeA
35000
Q70Y21
isoenzyme TsFaeB
35000
x * 35000, SDS-PAGE
35000
x * 29286, calculated from the deduced amino acid sequence, x * 35000, SDS-PAGE, x * 35040, average mass from mass spertrometry
35000
Q70Y21
x * 35000, type A and B enzymes, SDS-PAGE
35000
-
x * 35000, mutant enzyme N79A, SDS-PAGE
35000
-
x * 35000, mutant enzyme N79Q, SDS-PAGE
35000
-
x * 53000, chimeric enzyme, SDS-PAGE. x * 35000, native enzyme, SDS-PAGE
36000
-
-
36000
-
x * 36000, SDS-PAGE
36000
x * 36000, SDS-PAGE
36000
x * 36000, SDS-PAGE
36000
-
x * 36000, recombinant enzyme, SDS-PAGE, x * 28300, about, sequence calculation
37000
-
37000
-
x * 37000, wild-type enzyme, SDS-PAGE
40000
-
40000
-
mass spectrometry
40000
-
x * 40000, recombinant enzyme, SDS-PAGE, x * 28164, sequence calculation
43000
-
native PAGE
43000
-
x * 43000, SDS-PAGE, partially purified enzyme
45000
-
-
45000
-
domain XynZ, expected product of the 1,203-bp gene
53000
-
53000
-
x * 53000, chimeric enzyme, SDS-PAGE. x * 35000, native enzyme, SDS-PAGE
55000
-
55000
-
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE
55000
-
2 * 55000, recombinant deglycosylated enzyme, SDS-PAGE, 2 * 67000, recombinant glycosylated enzyme, SDS-PAGE
55340
Q70Y21
1 * 55340, calculated from the deduced amino acid sequence, 1 * 72000, SDS-PAGE
55340
Q70Y21
x * 55340, type C enzyme, calculated from the deduced amino acid sequence, x * 66000, smeared band on SDS-PAGE
56000
-
-
56000
-
x * 56000, deglycosylated enzyme, SDS-PAGE
57000
-
-
57500
-
-
57500
-
gel filtration, denaturing PAGE
61000
-
FaeB
61000
-
x * 61000, SDS-PAGE
61000
-
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE
62000
-
63000
-
-
65000
-
-
65000
-
gel filtration, non-denaturing PAGE
66000
Q70Y21
-
66000
Q70Y21
isoenzyme TsFaeC
66000
Q70Y21
x * 55340, type C enzyme, calculated from the deduced amino acid sequence, x * 66000, smeared band on SDS-PAGE
67000
gel filtration
67000
-
2 * 55000, recombinant deglycosylated enzyme, SDS-PAGE, 2 * 67000, recombinant glycosylated enzyme, SDS-PAGE
69000
-
-
69000
-
FAE-I, denaturing and nondenaturing PAGE, gel filtration
74000
-
74000
x * 74000, SDS-PAGE of intact protein, deglycosylated protein has a mass of 60000 Da
75000
-
p-coumaroyl esterase, SDS-PAGE
75000
-
gel filtration, in solution
75000
2 * 75000, SDS-PAGE, native mass by gel filtration
75000
-
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE
75800
-
75800
-
2 * 75800, CinnAE, SDS-PAGE
76000
-
76000
x * 76000, SDS-PAGE
84000
-
1 * 84000, hexahistidine fusion protein, SDS-PAGE, exists either as monomer or homodimer in solution
84000
-
2 * 84000, gel filtration, exists either as monomer or homodimer in solution