Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.73 extracted from

  • Castanares, A.; Wood, T.M.
    Purification and characterization of a feruloyl/p-coumaroyl esterase from solid-state cultures of the aerobic fungus Penicillium pinophilum (1992), Biochem. Soc. Trans., 20, 275S.
    View publication on PubMed

Application

Application Comment Organism
degradation the esterase capable to release phenolic acids from intact polymers, degradation may be of interest for industries wishing to effect the controlled degradation of plant cell walls Talaromyces pinophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.139
-
methyl ferulate
-
Talaromyces pinophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Talaromyces pinophilus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
SDS-PAGE Talaromyces pinophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl xylooligosaccharide + H2O Talaromyces pinophilus
-
ferulic acid + xylooligosaccharide
-
?
feruloyl-polysaccharide + H2O Schizophyllum commune
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Talaromyces pinophilus
-
ferulate + polysaccharide
-
?
feruloyloligosaccharide cell wall fragments + H2O Schizophyllum commune
-
ferulic acid + oligosaccharide cell wall fragments
-
?

Organism

Organism UniProt Comment Textmining
Schizophyllum commune
-
-
-
Talaromyces pinophilus
-
CMI 87160 ii
-

Purification (Commentary)

Purification (Comment) Organism
-
Talaromyces pinophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.6
-
-
Talaromyces pinophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylxylan + H2O
-
Talaromyces pinophilus acetic acid + ?
-
?
de-starched wheat bran + H2O
-
Schizophyllum commune ferulic acid + ?
-
?
de-starched wheat bran + H2O
-
Talaromyces pinophilus ferulic acid + ?
-
?
feruloyl xylooligosaccharide + H2O
-
Talaromyces pinophilus ferulic acid + xylooligosaccharide
-
?
feruloyl-polysaccharide + H2O
-
Schizophyllum commune ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Talaromyces pinophilus ferulate + polysaccharide
-
?
feruloyloligosaccharide cell wall fragments + H2O
-
Schizophyllum commune ferulic acid + oligosaccharide cell wall fragments
-
?
p-coumaric acid methyl ester + H2O
-
Talaromyces pinophilus p-coumaric acid + methanol
-
?
p-nitrophenyl acetate + H2O
-
Talaromyces pinophilus p-nitrophenol + acetate
-
?
xylan polysaccharide + H2O
-
Talaromyces pinophilus ferulic acid + p-coumaric acid
-
?

Subunits

Subunits Comment Organism
monomer 1 * 57000, SDS-PAGE Talaromyces pinophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
methyl ferulate as substrate Talaromyces pinophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 55
-
Talaromyces pinophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 60 purified enzyme is stable over the range 37-55°C for up to 8h, but loses activity rapidly at temperatures above 60°C Talaromyces pinophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
methyl ferulate as substrate Talaromyces pinophilus