3.1.1.73: feruloyl esterase
This is an abbreviated version!
For detailed information about feruloyl esterase, go to the full flat file.
Reaction
+
=
+
Synonyms
4-hydroxy-3-methoxycinnamic acid esterase, A.O.1, A.O.11, A.O.12, A.O.13, A.O.2, A.O.3, A.O.4, A.O.5, A.O.6, A.O.7, A.O.8, A.O.9, acetyl/ferulic acid esterase, AfFaeA, AN1772.2, AnFAE, AnFaeA, AnFAEB, AO090701000884, AoFaeB, AoFaeC, AtFAE-1, AtFAE-2, AtFAE-3, AuFaeA, AusFaeA, AwFAE, AwFAEA, BioH, carboxylic ester hydrolase, CE1, cellulosome multi-enzyme complex, cellulosome xylanase Z feruloyl esterase, CinI, CinII, cinnAE, cinnamic acid esterase, cinnamic acid hydrolase, cinnamic acid hydrolases, cinnamoyl ester hydrolase, Cinnamoyl esterase, CjXYLD, Est1, Est1E, EstA, esterase A, EstF27, FA esterase, FAE, FAE B, Fae-1, FAE-2, FAE-A, FAE-B, FAE-C, FAE-D, FAE-I, FAE-II, FAE-III, FAE-PL, FAE1, FAE125, Fae1A, FAE2, FAE3, FAE4, FAE5, FAE6, FAE68, FAE7, FAE7262, FAEA, FAEA1, FAEA2, FAEB, FaeB1, FAEB2, FaeC, FaeD, FaeD-3.544, FaeD1, FaeD2, FaeI, FaeT, FAE_XynZ, FE, FeE, Fee1B, ferulic acid esterase, ferulic acid esterase A, ferulic acid esterase B, ferulic acid esterase C, ferulic acid esterase D, feruloyl esterase, feruloyl esterase A, feruloyl esterase B, feruloyl/p-coumaroyl esterase, Feruloylesterase, FoFAE, FoFAE-I, FoFAE-II, FoFaeA, FoFaeB, FoFaeI, FoFaeII, hemicellulase acessory enzymes, hydroxycinnamoyl esterase, KX091144, Lp_0796, More, NcFaeB, NcFaeD-3.544, PeFaeA, PfFaeB, phenolic acid esterase, PSHAa enzyme, PSHAa1385, R18, SCHCODRAFT_60993, SCHCODRAFT_61055, StFAE, StFAE-A, StFaeB, StFaeC, TH2-18, TsFaeA, TsFaeB, TsFaeC, Tvms10a, Tvmz2a, type A FAE, type A feruloyl esterase, type B FAE, type B ferulic acid esterase, XLYD, XM 001217492, xylan-degrading enzyme system, xylanase 10, xylanase 10B, xylanase Z, xylanase-ferulic acid esterase, XYLD, XYLD esterase, Xyn10B, Xyn10D-Fae1A, XynZ
ECTree
Inhibitors
Inhibitors on EC 3.1.1.73 - feruloyl esterase
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2-ethyl-5-phenylisoxazolium-3'-sulfonate
-
-
acetonitrile
strong inhibition
aminoethylbenzenesulfonylfluoride
-
-
caffeic acid methyl ester
-
diethyl ether
strong inhibition
diethyl-2-(4-hydroxy-3-methoxyphenyl)ethenephosphonate
-
diisopropyl fluorophosphate
-
inhibits completely
diisopropyl fluorophosphates
-
inhibits feruloyl esterase activity completely
diisopropylfluorophosphate
-
-
dithiothreitol
-
slight inhibition
ferulic acid
-
end-product inhibition
iodoacetamide
-
50 mM, 45% inhibition
isobutyl alcohol
strong inhibition
methyl 5-N-arabinoferulamide
-
methyl-3,4-dimethoxy cinnamate
product inhibition
Monoiodoacetic acid
-
1 mM inhibits 5% of the activity
Na+
1 mM, 95% of initial activity
p-bromophenacyl bromide
-
-
p-chloromercuribenzoic acid
-
1 mM inhibits 10% of the activity
p-chloromercuric benzoate
-
50 mM, 67% inhibition
phenylmethylsulfonyl fluoride
tert-butyl methyl ether
strong inhibition
Toluene
strong inhibition
2-mercaptoethanol
-
1 mM, 83% of initial activity
acetone
-
-
acetone
strong inhibition
Al3+
-
5 mM, 90% residual activity
Al3+
-
40% inhibition at 5 mM
Al3+
-
23% inhibition at 2.5 mM, 45% inhibition at 5 mM
Al3+
8% inhibition at 1 mM, 25% inhibition at 10 mM
Ba2+
-
inhibits feruloyl esterase, no effect on p-coumaroyl esterase
Ba2+
10 mM, 82% residual activtiy
Ca2+
1 mM, 93% of initial activity
Ca2+
-
5 mM, 91% residual activity
Ca2+
10 mM, 87% residual activtiy
Ca2+
-
60% inhibition at 5 mM
Cd2+
-
5 mM, 84% residual activity
Cd2+
-
60% inhibition at 5 mM
Cd2+
-
31% inhibition at 5 mM
Co2+
-
1 mM inhibits 10% of the activity
Co2+
10 mM, 74% residual activtiy
Co2+
no activation at 1 mM, 11% at 5 mM, but 30% inhibition at 10 mM
Cu2+
-
1 mM inhibits 20% of the activity
Cu2+
-
1 mM, 55% of initial activity
Cu2+
1 mM, 55.6% of initial activity; 1 mM, 57.6% of initial activity; 1 mM, 8.5% of initial activity
Cu2+
-
5 mM, 88% residual activity
Cu2+
-
5 mM, almost complete inhibition
Cu2+
10 mM, no residual activtiy
Cu2+
-
40% inhibition at 5 mM
Cu2+
-
20% inhibition at 5 mM, 37% at 10 mM
Cu2+
70% inhibition at 1 mM, complete inhibition at 5-10 mM
DMSO
-
-
EDTA
-
ethanol
-
-
ethanol
strong inhibition
ethanol
-
slightly inhibitory at a concentration of 10%
Fe2+
-
-
Fe2+
-
1 mM inhibits 80% of the activity; inhibits 80% of the activity
Fe2+
1 mM, 61% of initial activity; 1 mM, 71% of initial activity; 1 mM, 84% of initial activity
Fe2+
-
5 mM, no residual activity
Fe2+
10 mM, no residual activtiy
Fe2+
-
complete inhibition at 5 mM
Fe3+
-
Fe3+
-
5 mM, complete inactivation
Fe3+
-
5 mM, almost complete inhibition
Fe3+
10 mM, no residual activtiy
Fe3+
-
strong inhibition of isozyme R18, weak inhibition of isozyme R43
Fe3+
23% activation at 1 mM, 13% at 5 mM, 78% inhibition at 10 mM
Hg2+
-
-
Hg2+
-
1 mM inhibits completely; inhibits feruloyl esterase activity completely
Hg2+
-
1 mM, 10% of initial activity
Hg2+
complete inhibition at 1 mM; complete inhibition at 1 mM
Hg2+
-
5 mM, no resiudal activity
Hg2+
-
60% inhibition at 1.25 mM, about 70% inhibition at 2.5-10 mM
K+
-
K+
1 mM, 88% of initial activity
K+
10 mM, 84% residual activtiy
K+
-
40% inhibition at 5 mM
K+
-
10% inhibition at 5 mM, 20% at 10 mM
methanol
-
-
methanol
complete inhibition
Mg2+
-
Mg2+
1 mM, 92% of initial activity; 1 mM, 94% of initial activity
Mg2+
10 mM, 84% residual activtiy
Mg2+
-
40% inhibition at 5 mM
Mg2+
-
8.5% inhibition at 5 mM, 22% at 10 mM
Mn2+
-
1 mM inhibits 10% of the activity
Mn2+
1 mM, 83% of initial activity
Mn2+
-
5 mM, 83% residual activity
Mn2+
10 mM, 66% residual activtiy
Mn2+
-
69% residual activity
Mn2+
-
60% inhibition at 5 mM
Mn2+
10% inhibition at 1 mM, 26% inhibition at 10 mM
Ni2+
-
1 mM inhibits 5% of the activity
Ni2+
1 mM, 15.1% of initial activity; 1 mM, 44.5% of initial activity; 1 mM, 63% of initial activity
Pb2+
-
20% inhibition at 5 mM
Pb2+
-
complete inhibition at 2.5 mM
phenylmethylsulfonyl fluoride
-
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
1 mM inhibits completely; inhibits feruloyl esterase activity completely
PMSF
-
1 mM, no residual activity
PMSF
complete inhibition at 1 mM; complete inhibition at 1 mM
PMSF
1 mM, 15% of initial activity; 1 mM, 55% of initial activity; 1 mM, 60% of initial activity
PMSF
50-100% inhibition at 1 mM dependent on the buffer system
Propanol
-
-
SDS
strong inhibition
SDS
95% inhibition at 5 mM
Zn2+
-
inhibits feruloyl esterase, no effect on p-coumaroyl esterase
Zn2+
1 mM, 14.3% of initial activity; 1 mM, 67.7% of initial activity; 1 mM, 74% of initial activity
Zn2+
10 mM, 68% residual activtiy
Zn2+
-
67% residual activity
Zn2+
-
40% inhibition at 5 mM
Zn2+
-
47% inhibition at 10 mM
Zn2+
-
strong inhibition of both isozymes
Zn2+
24% inhibition at 1 mM, 61% inhibition at 10 mM
additional information
Fae1A is not affected by the addition of EDTA
-
additional information
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Fae1A is not affected by the addition of EDTA
-
additional information
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1 mM Ag2+, Ca2+, Mg2+, Zn2+, EDTA and SDS have no effect on activity
-
additional information
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organic solvent inhibition kinetics with recombinant enzyme, overview
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additional information
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not inhibiting/activating: K+ or Zn2+ at 5 mM
-
additional information
the enzyme activity is not significantly affected by FeCl2, NiCl2, MnCl2, cysteine, CaCl2, MgCl2, DMSO, urea, and Triton X-100
-
additional information
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the enzyme activity is not significantly affected by FeCl2, NiCl2, MnCl2, cysteine, CaCl2, MgCl2, DMSO, urea, and Triton X-100
-
additional information
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MIC values of cell growth inhibitors, overview. No inhibition by 4-OH-phenylacetic acid and methyl syringate
-
additional information
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organic solvent inhibition kinetics with recombinant enzyme, overview
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additional information
no inhibition by EDTA
-
additional information
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no inhibition by EDTA
-
additional information
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organic solvent inhibition kinetics with recombinant enzyme, overview
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