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Literature summary for 3.1.1.73 extracted from
- Production and biochemical characterization of a type B ferulic acid esterase from Streptomyces ambofaciens (2009), Can. J. Microbiol., 55, 729-738.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE, partially purified enzyme | Streptomyces ambofaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in Streptomyces lividans | - |
- |
- |
| no activity in Streptomyces lividans TK24 | - |
- |
- |
| no activity in Streptomyces pristinaespiralis | - |
- |
- |
| no activity in Streptomyces pristinaespiralis Pr11 | - |
- |
- |
| Streptomyces ambofaciens | - |
strain ATCC 23877 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially purified by 50% ammonium sulfate precipitation and dialysis, 3.6fold with a yield of 25% | Streptomyces ambofaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40 | - |
with destarched maize bran (1%) as the carbon source, at pH 6.5 and 40°C | Streptomyces ambofaciens |
| 40 | - |
with rape cattle cake (1%) as the carbon source, at pH 6.5 and 40°C | Streptomyces ambofaciens |
| 60 | - |
with destarched wheat bran (1%) as the carbon source and in the presence of 0.17 g/L ferulic acid, at pH 6.5 and 40°C | Streptomyces ambofaciens |
| 100 | - |
FAE activity decreases up to 24 h of culture and is maintained at 0.1 mU/mg protein with destarched wheat bran (1%) as the carbon source, at pH 6.5 and 40°C | Streptomyces ambofaciens |
| 160 | - |
with methyl cinnamate as substrate, at pH 6.5 and 40°C | Streptomyces ambofaciens |
| 200 | - |
with destarched wheat bran (1%) as the carbon source and in the presence of 0.03 g/L ferulic acid, at pH 6.5 and 40°C | Streptomyces ambofaciens |
| 200 | - |
with methyl ferulate as substrate, at pH 6.5 and 40°C | Streptomyces ambofaciens |
| 210 | - |
with oat spelt xylan (1%) as the carbon source, at pH 6.5 and 40°C | Streptomyces ambofaciens |
| 220 | - |
with destarched wheat bran (1%) as the carbon source or ethyl cinnamate as substrate, at pH 6.5 and 40°C | Streptomyces ambofaciens |
| 300 | - |
with methyl p-coumarate as substrate, at pH 6.5 and 40°C | Streptomyces ambofaciens |
| 600 | - |
after 14 h of culture with destarched wheat bran (1%) as the carbon source, at pH 6.5 and 40°C | Streptomyces ambofaciens |
| 800 | - |
3.6fold purified enzyme | Streptomyces ambofaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl cinnamate + H2O | highest activity | Streptomyces ambofaciens | cinnamic acid + methanol | - |
? |  |
| methyl ferulate + H2O | highest activity | Streptomyces ambofaciens | ferulic acid + methanol | - |
? | |
| methyl p-coumarate + H2O | highest activity | Streptomyces ambofaciens | p-coumaric acid + methanol | - |
? | |
| additional information | no activity with butyl cinnamate, methyl caffeate, methyl sinapate, methyl vanillate and methyl syringate | Streptomyces ambofaciens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE, partially purified enzyme | Streptomyces ambofaciens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAE | - |
Streptomyces ambofaciens |
| ferulic acid esterase | - |
Streptomyces ambofaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified enzyme | Streptomyces ambofaciens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | purified enzyme | Streptomyces ambofaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
after 2 h at 37°C, the FAE specific activity is 60% reduced | Streptomyces ambofaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
purified enzyme | Streptomyces ambofaciens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | purified enzyme | Streptomyces ambofaciens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Streptomyces ambofaciens | no FAE activity in the presence of 0.1% ethyl ferulate in the culture medium. Low FAE activity in the absence of lignocellulosic substrate, maybe due to a constitutive synthesis of the FAE | down |
| Streptomyces ambofaciens | induction of the FAE synthesis is not related to the ferulic acid content in the substrate used | additional information |
| Streptomyces ambofaciens | maximal level of activity in the presence of destarched wheat bran as the sole carbon source. 1% (m/v) of destarched wheat bran is the optimal concentration to induce its production. With this inducer, no ferulic acid dimers are released from the cell wall by the produced FAE. Destarched maize bran is capable of inducing the synthesis of a small amount of FAE. Maximal level of activity in the presence of oat spelt xylan as the sole carbon source. Rape cattle cake (Brassica napus), which does not contain esterified ferulic acid, is capable of inducing the synthesis of a small amount of FAE. With beet pectins or with white dextrins, FAE activity is detected when a drop of culture supernatant is deposited on the agar medium containing ethyl ferulate, but this activity is too weak to be quantified | up |
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