Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 40% inhibition at 5 mM | Pleurotus giganteus | |
Ca2+ | 60% inhibition at 5 mM | Pleurotus giganteus | |
Cd2+ | 60% inhibition at 5 mM | Pleurotus giganteus | |
Cu2+ | 40% inhibition at 5 mM | Pleurotus giganteus | |
K+ | 40% inhibition at 5 mM | Pleurotus giganteus | |
Mg2+ | 40% inhibition at 5 mM | Pleurotus giganteus | |
Mn2+ | 60% inhibition at 5 mM | Pleurotus giganteus | |
Pb2+ | 20% inhibition at 5 mM | Pleurotus giganteus | |
Zn2+ | 40% inhibition at 5 mM | Pleurotus giganteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
methyl ferulate | pH 5.0, 37°C | Pleurotus giganteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
x * 61000, SDS-PAGE | Pleurotus giganteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pleurotus giganteus | the single purified enzyme brings about the release of ferulic acid from wheat bran and corn bran, and the amount released from wheat bran rises to 51.9% (of the total amount) by the synergistic action of xylanase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus giganteus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 130.8fold from fruiting bodies by two different steps of anion exchange chromatography, and gel filtration | Pleurotus giganteus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | fresh | Pleurotus giganteus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
170 | - |
purified enzyme, pH 5.0, 37°C | Pleurotus giganteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Pleurotus giganteus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Pleurotus giganteus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Pleurotus giganteus | 4-nitrophenol + caproate | - |
? | |
methyl 4-coumarate + H2O | - |
Pleurotus giganteus | 4-coumarate + methanol | - |
? | |
methyl caffeate + H2O | - |
Pleurotus giganteus | caffeic acid + methanol | - |
? | |
methyl ferulate + H2O | - |
Pleurotus giganteus | ferulate + methanol | - |
? | |
additional information | the single purified enzyme brings about the release of ferulic acid from wheat bran and corn bran, and the amount released from wheat bran rises to 51.9% (of the total amount) by the synergistic action of xylanase | Pleurotus giganteus | ? | - |
? | |
additional information | no activity with 4-nitrophenyl palmitate and 4-nitrophenyl stearate | Pleurotus giganteus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, SDS-PAGE | Pleurotus giganteus |
Synonyms | Comment | Organism |
---|---|---|
FAE | - |
Pleurotus giganteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pleurotus giganteus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 90 | 68% of maximal activity at 20°C, 45% at 90°C, inactivation at 100°C | Pleurotus giganteus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
inactivation | Pleurotus giganteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Pleurotus giganteus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 7 | 60% of maximal activity at pH 2.0, maximal activity at pH 4.0, very low activity at pH 7.0, inactive at pH 8.0 | Pleurotus giganteus |