Cloned (Comment) | Organism |
---|---|
gene Ft3-7, DNA and amino acid sequence determination and analysis, expression of His-tagged feruoyl esterase in Escherichia coli strain BL21 | uncultured bacterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | uncultured bacterium | ability of the purified Ft3-7 protein to release phenolic compounds from crude plant cell wall materials in the form of crop, e.g. wheat bran and spent grain, release of ferulic acid and diferulic acid together with xylanase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | - |
gene Ft3-7 amplified from a symbiont in the gut of Coptotermes formosanus | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged feruoyl esterase from Escherichia coli strain BL21 by nickel affinity chromatography | uncultured bacterium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gut | - |
uncultured bacterium | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.6 | - |
recombinant enzyme, with substrate ethyl ferulate, pH 7.0, 37°C | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl ferulate + H2O | - |
uncultured bacterium | ethanol + ferulate | - |
? | |
additional information | ability of the purified Ft3-7 protein to release phenolic compounds from crude plant cell wall materials in the form of crop, e.g. wheat bran and spent grain, release of ferulic acid and diferulic acid together with xylanase | uncultured bacterium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAE | - |
uncultured bacterium |
More | the enzyme belongs to the type-D sub-class of ferulic acid esterases | uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme is stable below 45°C, it quickly loses activity above 50°C | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant enzyme | uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.9 | the enzyme shows a broad optimum | uncultured bacterium |