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Literature summary for 3.1.1.73 extracted from

  • Rumbold, K.; Biely, P.; Mastihubova, M.; Gudelj, M.; Gubitz, G.; Robra, K.H.; Prior, B.A.
    Purification and properties of a feruloyl esterase involved in lignocellulose degradation by Aureobasidium pullulans (2003), Appl. Environ. Microbiol., 69, 5622-5626.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.73

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 methyl p-coumarate 30°C Aureobasidium pullulans
0.05
-
 methyl ferulate 30°C Aureobasidium pullulans
0.1
-
 methyl caffeate 30°C Aureobasidium pullulans
0.14
-
 methyl sinapinate 30°C Aureobasidium pullulans
0.23
-
 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside 30°C Aureobasidium pullulans
0.27
-
 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside 30°C Aureobasidium pullulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aureobasidium pullulans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
210000
-
 gel filtration Aureobasidium pullulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl polysaccharide + H2O Aureobasidium pullulans involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
feruloyl polysaccharide + H2O Aureobasidium pullulans NRRL Y 2311-1 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
lignocellulose + H2O Aureobasidium pullulans
-
 ferulic acid + ?
-
 ?
wheat bran + H2O Aureobasidium pullulans
-
 ferulic acid + ?
-
 ?
wheat bran + H2O Aureobasidium pullulans NRRL Y 2311-1
-
 ferulic acid + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aureobasidium pullulans
-
 produces feruloyl esterase activity when grown on birchwood xylan
-
Aureobasidium pullulans NRRL Y 2311-1
-
 produces feruloyl esterase activity when grown on birchwood xylan
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 48% glycosylation Aureobasidium pullulans

Purification (Commentary)

Purification (Comment) Organism
-
 Aureobasidium pullulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 p-nitrophenyl butyrate as substrate, 30°C Aureobasidium pullulans
12.9
-
 methyl sinapinate as substrate, 30°C Aureobasidium pullulans
21.6
-
 methyl ferulate as substrate, 30°C Aureobasidium pullulans
30.4
-
 methyl caffeate as substrate, 30°C Aureobasidium pullulans
31.8
-
 p-nitrophenyl acetate as substrate, 30°C Aureobasidium pullulans
35.3
-
 methyl p-coumarate as substrate, 30°C Aureobasidium pullulans
35.9
-
 O-{5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl}-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose as substrate, 30°C Aureobasidium pullulans
68.8
-
 purified enzyme, 30°C Aureobasidium pullulans
71.1
-
 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside as substrate, 30°C Aureobasidium pullulans
73.2
-
 4-nitrophenyl 4-O-trans-feruloylxylopyranoside as substrate, 30°C Aureobasidium pullulans
74.6
-
 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside as substrate, 30°C Aureobasidium pullulans

Storage Stability

Storage Stability Organism
4°C, crude culture filtrate, 6 months, 20% loss of activity Aureobasidium pullulans
4°C, partially purified enzyme, 1 month, 50% loss of activity Aureobasidium pullulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O 2-O and 5-O linked ferulate is hydrolyzed equally well Aureobasidium pullulans ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
 ?
4-nitrophenyl 4-O-trans-feruloylxylopyranoside + H2O
-
 Aureobasidium pullulans ferulic acid + ?
-
 ?
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O 2-O and 5-O linked ferulate is hydrolyzed equally well Aureobasidium pullulans ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
 ?
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aureobasidium pullulans ferulic acid + ?
-
 ?
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aureobasidium pullulans NRRL Y 2311-1 ferulic acid + ?
-
 ?
lignocellulose + H2O
-
 Aureobasidium pullulans ferulic acid + ?
-
 ?
methyl caffeate + H2O
-
 Aureobasidium pullulans caffeic acid + methanol
-
 ?
methyl caffeate + H2O
-
 Aureobasidium pullulans NRRL Y 2311-1 caffeic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Aureobasidium pullulans ferulic acid + methanol
-
 ?
methyl p-coumarate + H2O highest activity of all substrates tested Aureobasidium pullulans p-coumaric acid + methanol
-
 ?
methyl sinapinate + H2O
-
 Aureobasidium pullulans sinapic acid + methanol
-
 ?
methyl sinapinate + H2O
-
 Aureobasidium pullulans NRRL Y 2311-1 sinapic acid + methanol
-
 ?
O-[5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl]-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + H2O
-
 Aureobasidium pullulans ferulic acid + alpha-L-arabinofuranosyl-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose
-
 ?
p-nitrophenyl acetate + H2O
-
 Aureobasidium pullulans ?
-
 ?
p-nitrophenyl butyrate + H2O poor substrate Aureobasidium pullulans ?
-
 ?
p-nitrophenyl butyrate + H2O poor substrate Aureobasidium pullulans NRRL Y 2311-1 ?
-
 ?
wheat bran + H2O
-
 Aureobasidium pullulans ferulic acid + ?
-
 ?
wheat bran + H2O
-
 Aureobasidium pullulans NRRL Y 2311-1 ferulic acid + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 210000, SDS-PAGE, native mass by gel filtration, 110000 Da estimated by SDS-PAGE for the deglycosylated form Aureobasidium pullulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aureobasidium pullulans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
 79% of maximum activity Aureobasidium pullulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 3.7 methyl sinapinate 30°C Aureobasidium pullulans
15.3
-
 methyl ferulate 30°C Aureobasidium pullulans
30
-
 methyl caffeate 30°C Aureobasidium pullulans
31.1
-
 methyl p-coumarate 30°C Aureobasidium pullulans
65.5
-
 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside 30°C Aureobasidium pullulans
70.2
-
 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside 30°C Aureobasidium pullulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
-
 Aureobasidium pullulans

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5 50% of activity at pH 4 and 8.5 Aureobasidium pullulans

pI Value

Organism Comment pI Value Maximum pI Value
Aureobasidium pullulans isoelectric focusing
-
 6.5