3.1.1.73: feruloyl esterase
This is an abbreviated version!
For detailed information about feruloyl esterase, go to the full flat file.
Reaction
+
=
+
Synonyms
4-hydroxy-3-methoxycinnamic acid esterase, A.O.1, A.O.11, A.O.12, A.O.13, A.O.2, A.O.3, A.O.4, A.O.5, A.O.6, A.O.7, A.O.8, A.O.9, acetyl/ferulic acid esterase, AfFaeA, AN1772.2, AnFAE, AnFaeA, AnFAEB, AO090701000884, AoFaeB, AoFaeC, AtFAE-1, AtFAE-2, AtFAE-3, AuFaeA, AusFaeA, AwFAE, AwFAEA, BioH, carboxylic ester hydrolase, CE1, cellulosome multi-enzyme complex, cellulosome xylanase Z feruloyl esterase, CinI, CinII, cinnAE, cinnamic acid esterase, cinnamic acid hydrolase, cinnamic acid hydrolases, cinnamoyl ester hydrolase, Cinnamoyl esterase, CjXYLD, Est1, Est1E, EstA, esterase A, EstF27, FA esterase, FAE, FAE B, Fae-1, FAE-2, FAE-A, FAE-B, FAE-C, FAE-D, FAE-I, FAE-II, FAE-III, FAE-PL, FAE1, FAE125, Fae1A, FAE2, FAE3, FAE4, FAE5, FAE6, FAE68, FAE7, FAE7262, FAEA, FAEA1, FAEA2, FAEB, FaeB1, FAEB2, FaeC, FaeD, FaeD-3.544, FaeD1, FaeD2, FaeI, FaeT, FAE_XynZ, FE, FeE, Fee1B, ferulic acid esterase, ferulic acid esterase A, ferulic acid esterase B, ferulic acid esterase C, ferulic acid esterase D, feruloyl esterase, feruloyl esterase A, feruloyl esterase B, feruloyl/p-coumaroyl esterase, Feruloylesterase, FoFAE, FoFAE-I, FoFAE-II, FoFaeA, FoFaeB, FoFaeI, FoFaeII, hemicellulase acessory enzymes, hydroxycinnamoyl esterase, KX091144, Lp_0796, More, NcFaeB, NcFaeD-3.544, PeFaeA, PfFaeB, phenolic acid esterase, PSHAa enzyme, PSHAa1385, R18, SCHCODRAFT_60993, SCHCODRAFT_61055, StFAE, StFAE-A, StFaeB, StFaeC, TH2-18, TsFaeA, TsFaeB, TsFaeC, Tvms10a, Tvmz2a, type A FAE, type A feruloyl esterase, type B FAE, type B ferulic acid esterase, XLYD, XM 001217492, xylan-degrading enzyme system, xylanase 10, xylanase 10B, xylanase Z, xylanase-ferulic acid esterase, XYLD, XYLD esterase, Xyn10B, Xyn10D-Fae1A, XynZ
ECTree
KCat KM Value
KCat KM Value on EC 3.1.1.73 - feruloyl esterase
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4.1 - 242
4-nitrophenyl acetate
109
4-nitrophenyl butanoate
1286
4-nitrophenyl butyrate
pH 6.8, 40°C
30 - 76.6
4-nitrophenyl ferulate
8.6 - 12.7
alpha-naphthyl propionate
8.64 - 759
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose
57.3 - 58.2
chlorogenic acid
0.00062 - 1627
ethyl ferulate
35.68 - 37.25
ferulic acid 4-nitrophenyl ester
2
ferulic acid ethyl ester
-
pH 8.8, 40°C
0.9 - 4.25
ferulic acid methyl ester
29.7 - 51.3
feruloyl arabino-xylo-oligosaccharides
recombinant His-tagged enzyme, pH 7.0, 37°C
-
0.88 - 681.5
methyl 4-coumarate
210 - 257.2
methyl 4-hydroxybenzoate
2.6 - 4352
methyl caffeate
0.00035 - 5226
methyl ferulate
1.21 - 4673
methyl p-coumarate
6.3 - 537
methyl sinapate
0.0006 - 2078
methyl sinapinate
3.2 - 780
n-decyl ferulate
5.6 - 906
n-hexyl ferulate
2.6 - 452
n-propyl ferulate
0.81 - 2.7
p-coumaric acid methyl ester
120
p-nitrophenyl acetate
-
in 100 mM phosphate buffer, pH 7.2, at 37°C
100
p-nitrophenyl butyrate
-
in 100 mM phosphate buffer, pH 7.2, at 37°C
3.23
sinapic acid methyl ester
pH 5.0, 40°C
128
1-naphthyl acetate
Thermochaetoides dissita
-
pH 6.0, 55°C
128
1-naphthyl acetate
Thermochaetoides dissita
pH 6.0, 55°C
4.1
4-nitrophenyl acetate
pH 6.0, 50°C
86
4-nitrophenyl acetate
Thermochaetoides dissita
pH 6.0, 55°C
242
4-nitrophenyl acetate
pH 6.8, 40°C
109
4-nitrophenyl butanoate
Thermochaetoides dissita
-
pH 6.0, 55°C
109
4-nitrophenyl butanoate
Thermochaetoides dissita
pH 6.0, 55°C
30
4-nitrophenyl ferulate
Thermochaetoides dissita
pH 6.0, 55°C
39.4
4-nitrophenyl ferulate
pH 6.8, 40°C
40.5
4-nitrophenyl ferulate
pH 6.0, 50°C
52.4
4-nitrophenyl ferulate
mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 40°C
76.6
4-nitrophenyl ferulate
mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 65°C
8.6
alpha-naphthyl propionate
mutant W144Y, pH 7.0, 37°C
10
alpha-naphthyl propionate
wild-type, pH 7.0, 37°C
12.7
alpha-naphthyl propionate
mutant W142F, pH 7.0, 37°C
8.64
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose
-
pH 5.0, 37°C
8.64
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose
pH 5.0, 37°C
759
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose
pH 6.0, 37°C
2.52
chlorogenate
pH 8.0, 40°C
4.32
chlorogenate
pH 8.0, 35°C
45.31
chlorogenate
pH 8.0, 30°C
1598
chlorogenate
pH 6.0, 37°C
57.3
chlorogenic acid
pH 7.5, 37°C
58.2
chlorogenic acid
pH 7.5, 37°C
0.00062
ethyl ferulate
wild-type, pH not specified in the publication, 50°C
0.0076
ethyl ferulate
pH not specified in the publication, 50°C
0.0177
ethyl ferulate
mutant 18PG, pH not specified in the publication, 50°C
1.23
ethyl ferulate
pH 8.0, 40°C
2.2
ethyl ferulate
-
pH 7.0, 40°C
5.48
ethyl ferulate
pH 8.0, 35°C
7.9
ethyl ferulate
pH 9.0, 30°C
11.72
ethyl ferulate
pH 8.0, 30°C
48
ethyl ferulate
pH 6.0, 40°C
164
ethyl ferulate
Thermochaetoides dissita
-
pH 6.0, 55°C
164
ethyl ferulate
Thermochaetoides dissita
pH 6.0, 55°C
206
ethyl ferulate
-
pH 7.0, 40°C
236
ethyl ferulate
pH 6.0, 40°C
260
ethyl ferulate
-
pH 6.0, 37°C
1071
ethyl ferulate
mutant W144Y, pH 7.0, 37°C
1627
ethyl ferulate
mutant W142F, pH 7.0, 37°C
4.3
ferulate
pH 6.4, 40°C, wild-type enzyme
6.5
ferulate
pH 6.4, 40°C, mutant D93G
34
ferulate
pH 6.4, 40°C, mutant D93G/S187F
41
ferulate
pH 6.4, 40°C, mutant S187F
35.68
ferulic acid 4-nitrophenyl ester
mutant A126C/N152C, pH 5.5, 40°C
37.25
ferulic acid 4-nitrophenyl ester
wild-type, pH 5.5, 40°C
0.9
ferulic acid methyl ester
-
pH 8.8, 40°C
2.81
ferulic acid methyl ester
-
pH 5.0, 37°C
4.25
ferulic acid methyl ester
pH 5.0, 40°C
0.88
methyl 4-coumarate
-
pH 5.5, 40°C, recombinant enzyme
681.5
methyl 4-coumarate
pH 6.8, 40°C
210
methyl 4-hydroxybenzoate
pH 7.5, 37°C
257.2
methyl 4-hydroxybenzoate
pH 7.5, 37°C
2.6
methyl caffeate
mutant W142F, pH 7.0, 37°C
3.2
methyl caffeate
mutant W144Y, pH 7.0, 37°C
99
methyl caffeate
Thermochaetoides dissita
-
pH 6.0, 55°C
99
methyl caffeate
Thermochaetoides dissita
pH 6.0, 55°C
99.6
methyl caffeate
pH 9.0, 30°C
275.1
methyl caffeate
pH 7.5, 37°C
310.6
methyl caffeate
pH 7.5, 37°C
675
methyl caffeate
wild-type, pH 6.0, 37°C
702
methyl caffeate
pH 6.0, 37°C
4352
methyl caffeate
-
pH 7.2, 30°C
0.00035
methyl ferulate
rumen microorganism
-
transesterification, immobilized enzyme, pH not specified in the publication, 40°C
0.00047
methyl ferulate
wild-type, pH not specified in the publication, 50°C
0.0038
methyl ferulate
rumen microorganism
-
transesterification, free enzyme, pH not specified in the publication, 40°C
0.00528
methyl ferulate
mutant 18PG, pH not specified in the publication, 50°C
0.0077
methyl ferulate
pH not specified in the publication, 50°C
0.14
methyl ferulate
mutant C235S, pH 6.4, 40°C
0.195
methyl ferulate
mutant L14F/T35I/K37I/T57I/T63I/A140T/Q121H/S163T/Q177H/V178A/Q185R/C235S, pH 6.4, 40°C
0.29
methyl ferulate
mutant D93G/S187F, pH 6.4, 40°C
0.305
methyl ferulate
mutant L14F/T35I/K37I/T57I/T63I/A140T/Q121H/S163T/Q177H/V178A/Q185R, pH 6.4, 40°C
0.69
methyl ferulate
pH 8.0, 40°C
2.81
methyl ferulate
pH 5.0, 37°C
3.2
methyl ferulate
-
pH 7.0, 40°C
4.32
methyl ferulate
pH 8.0, 35°C
5.02
methyl ferulate
-
pH 5.5, 40°C, recombinant enzyme
6.2
methyl ferulate
mutant W144Y, pH 7.0, 37°C
7.7
methyl ferulate
mutant W142F, pH 7.0, 37°C
10.5
methyl ferulate
recombinant His-tagged enzyme, pH 7.0, 37°C
10.57
methyl ferulate
pH 8.0, 30°C
15
methyl ferulate
rumen microorganism
-
hydrolysis, immobilized enzyme, pH not specified in the publication, 40°C
23.1
methyl ferulate
pH 6.8, 40°C
24.5
methyl ferulate
pH 6.0, 50°C
29
methyl ferulate
pH 6.0, 40°C
72.9
methyl ferulate
rumen microorganism
-
hydrolysis, free enzyme, pH not specified in the publication, 40°C
173
methyl ferulate
pH 6.0, 40°C
250
methyl ferulate
-
pH 7.0, 40°C
310
methyl ferulate
-
pH 6.0, 37°C
319.2
methyl ferulate
pH 7.5, 37°C
372.6
methyl ferulate
pH 7.5, 37°C
744
methyl ferulate
wild-type, pH 6.0, 37°C
1306
methyl ferulate
-
pH 5.0, 30°C
5226
methyl ferulate
-
pH 7.2, 30°C
1.21
methyl p-coumarate
pH 8.0, 40°C
5.5
methyl p-coumarate
mutant W142F, pH 7.0, 37°C
6.9
methyl p-coumarate
mutant W144Y, pH 7.0, 37°C
11.4
methyl p-coumarate
pH 8.0, 30°C
30.9
methyl p-coumarate
pH 8.0, 35°C
91
methyl p-coumarate
-
pH 5.0, 30°C
150
methyl p-coumarate
-
pH 6.0, 37°C
363
methyl p-coumarate
Thermochaetoides dissita
-
pH 6.0, 55°C
363
methyl p-coumarate
Thermochaetoides dissita
pH 6.0, 55°C
666.2
methyl p-coumarate
pH 7.5, 37°C
682
methyl p-coumarate
wild-type, pH 6.0, 37°C
696.3
methyl p-coumarate
pH 7.5, 37°C
925
methyl p-coumarate
pH 6.0, 37°C
4673
methyl p-coumarate
-
pH 7.2, 30°C
6.3
methyl sinapate
-
pH 6.0, 37°C
28.9
methyl sinapate
pH 6.8, 40°C
40
methyl sinapate
wild-type, pH 6.0, 37°C
537
methyl sinapate
-
pH 5.0, 30°C
0.0006
methyl sinapinate
pH not specified in the publication, 50°C
0.0006
methyl sinapinate
wild-type, pH not specified in the publication, 50°C
0.0427
methyl sinapinate
mutant 18PG, pH not specified in the publication, 50°C
3.48
methyl sinapinate
-
pH 5.5, 40°C, recombinant enzyme
6.7
methyl sinapinate
mutant W144Y, pH 7.0, 37°C
11.9
methyl sinapinate
mutant W142F, pH 7.0, 37°C
2078
methyl sinapinate
-
pH 7.2, 30°C
3.2
n-decyl ferulate
-
pH 7.0, 40°C
547
n-decyl ferulate
pH 6.0, 40°C
780
n-decyl ferulate
-
pH 7.0, 40°C
5.6
n-hexyl ferulate
-
pH 7.0, 40°C
225
n-hexyl ferulate
-
pH 7.0, 40°C
243
n-hexyl ferulate
pH 6.0, 40°C
906
n-hexyl ferulate
pH 6.0, 40°C
2.6
n-propyl ferulate
-
pH 7.0, 40°C
182
n-propyl ferulate
pH 6.0, 40°C
391
n-propyl ferulate
-
pH 7.0, 40°C
452
n-propyl ferulate
pH 6.0, 40°C
0.81
p-coumaric acid methyl ester
pH 5.0, 40°C
2.7
p-coumaric acid methyl ester
-
pH 8.8, 40°C