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Literature summary for 3.1.1.73 extracted from
- Expression and biochemical characterization of two novel feruloyl esterases derived from fecal samples of Rusa unicolor and Equus burchelli (2012), Gene, 500, 134-139.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21 | Equus quagga burchellii |
| DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21 | Rusa unicolor |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18600 | - |
x * 18600, recombinant His6-tagged enzyme, SDS-PAGE | Rusa unicolor |
| 31200 | - |
x * 31200, recombinant His6-tagged enzyme, SDS-PAGE | Equus quagga burchellii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equus quagga burchellii | F8SUI8 | gene tvmz2a, type-D feruloyl esterase | - |
| Rusa unicolor | F8SUI7 | gene tvms10a, type-D feruloyl esterase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Equus quagga burchellii |
| recombinant His6-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Rusa unicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.4 | - |
purified recombinant enzyme, pH 8.0, 38°C | Equus quagga burchellii |
| 16.6 | - |
purified recombinant enzyme, pH 7.0, 35°C | Rusa unicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl ferulate + H2O | - |
Equus quagga burchellii | ethanol + ferulate | - |
? |  |
| ethyl ferulate + H2O | - |
Rusa unicolor | ethanol + ferulate | - |
? | |
| additional information | the enzyme is capable of releasing ferulic acid and diferulic acid from untreated crude plant cell wall materials | Equus quagga burchellii | ? | - |
? | |
| additional information | the enzyme is capable of releasing ferulic acid and diferulic acid from untreated crude plant cell wall materials | Rusa unicolor | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 18600, recombinant His6-tagged enzyme, SDS-PAGE | Rusa unicolor |
| ? | x * 31200, recombinant His6-tagged enzyme, SDS-PAGE | Equus quagga burchellii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Tvms10a | - |
Rusa unicolor |
| Tvmz2a | - |
Equus quagga burchellii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Rusa unicolor |
| 38 | - |
- |
Equus quagga burchellii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 55 | activity range, 55% of maximal activity within the range of 24.6-44.5°C | Equus quagga burchellii |
| 10 | 55 | activity range, 55% of maximal activity within the range of 24.6-44.5°C | Rusa unicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant His-tagged enzyme, 60 min, completely stable | Equus quagga burchellii |
| 40 | - |
purified recombinant His-tagged enzyme, 60 min, completely stable | Rusa unicolor |
| 45 | - |
above, purified recombinant His-tagged enzyme, 60 min, rapid inactivation, loss of 68% activity after 5 min | Equus quagga burchellii |
| 45 | - |
above, purified recombinant His-tagged enzyme, 60 min, rapid inactivation, loss of 68% activity after 5 min | Rusa unicolor |
| 75 | - |
purified recombinant His-tagged enzyme, inactivation | Equus quagga burchellii |
| 75 | - |
purified recombinant His-tagged enzyme, inactivation | Rusa unicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rusa unicolor |
| 8 | - |
- |
Equus quagga burchellii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | activity range | Equus quagga burchellii |
| 4 | 9 | activity range | Rusa unicolor |
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