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Literature summary for 3.1.1.73 extracted from
- Characterization of a chimeric enzyme comprising feruloyl esterase and family 42 carbohydrate-binding module (2010), Appl. Microbiol. Biotechnol., 86, 155-161.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | chimeric enzyme FaeA/Aspergillus kawachii family 42 carbohydrate-binding module as an innovative enzymatic tool for biotechnological applications and biotransformation of plant biomass | Aspergillus awamori |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| chimeric gene of FaeA and Aspergillus kawachii family 42 carbohydrate-binding module (CBM42) cloned in pPICZalphaC vector. Native and chimeric enzyme comprising FaeA-CBM42 and the Saccharomyces cerevisiae alpha-factor secretion signal peptide expressed in Pichia pastoris strain GS115 | Aspergillus awamori |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 53000, chimeric enzyme, SDS-PAGE. x * 35000, native enzyme, SDS-PAGE | Aspergillus awamori |
| 53000 | - |
x * 53000, chimeric enzyme, SDS-PAGE. x * 35000, native enzyme, SDS-PAGE | Aspergillus awamori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus awamori | - |
- |
- |
| Aspergillus awamori NBRC4033 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and chimeric enzyme, by anion-exchange chromatography and gel filtration | Aspergillus awamori |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.098 micromol/min/nmol, native enzyme with methyl ferulate as substrate, at 37°C. 0.17 micromol/min/nmol, chimeric enzyme with methyl ferulate as substrate, at 37°C | Aspergillus awamori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-naphthyl butyrate + H2O | - |
Aspergillus awamori | alpha-naphthol + butyrate | - |
? |  |
| alpha-naphthyl butyrate + H2O | - |
Aspergillus awamori NBRC4033 | alpha-naphthol + butyrate | - |
? | |
| arabinoxylan + H2O | CBM42 binds to the arabinofuranose-containing side chain moiety of arabinoxylan. Release of ferulic acid from insoluble arabinoxylan by the chimeric enzyme is approximately 4fold higher than that achieved by FaeA alone after an incubation of 12 h. Chimeric enzyme and xylanase act synergistically for the degradation of arabinoxylan. Arabinoxylan degradation by xylanase is significant when arabinoxylan is pretreated with Aspergillus kawachii family 54 arabinofuranosidase (Abf54), FaeA, and chimeric FaeA-CBM42 than when it is not | Aspergillus awamori | ferulic acid + ? | - |
? | |
| arabinoxylan + H2O | CBM42 binds to the arabinofuranose-containing side chain moiety of arabinoxylan. Release of ferulic acid from insoluble arabinoxylan by the chimeric enzyme is approximately 4fold higher than that achieved by FaeA alone after an incubation of 12 h. Chimeric enzyme and xylanase act synergistically for the degradation of arabinoxylan. Arabinoxylan degradation by xylanase is significant when arabinoxylan is pretreated with Aspergillus kawachii family 54 arabinofuranosidase (Abf54), FaeA, and chimeric FaeA-CBM42 than when it is not | Aspergillus awamori NBRC4033 | ferulic acid + ? | - |
? | |
| methyl caffeate + H2O | - |
Aspergillus awamori | caffeic acid + methanol | - |
? | |
| methyl caffeate + H2O | - |
Aspergillus awamori NBRC4033 | caffeic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Aspergillus awamori | ferulic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Aspergillus awamori NBRC4033 | ferulic acid + methanol | - |
? | |
| methyl p-coumarate + H2O | - |
Aspergillus awamori | p-coumaric acid + methanol | - |
? | |
| methyl p-coumarate + H2O | - |
Aspergillus awamori NBRC4033 | p-coumaric acid + methanol | - |
? | |
| methyl sinapate + H2O | - |
Aspergillus awamori | sinapic acid + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, chimeric enzyme, SDS-PAGE. x * 35000, native enzyme, SDS-PAGE | Aspergillus awamori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAEA | - |
Aspergillus awamori |
| feruloyl esterase | - |
Aspergillus awamori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | native and chimeric enzymes retain more than 90% of their activity after incubation at 45°C and 50°C for 30 min, respectively | Aspergillus awamori |
| 55 | - |
after incubation at 55°C, the chimeric enzyme retains 40% of its activity, whereas, the native enzyme almost loses its activity | Aspergillus awamori |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | the chimeric enzyme does not degrade after an incubation of 12 h at room temperature | Aspergillus awamori |
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