Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | uncultured bacterium |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, hanging drop vapour diffusion, 0.002 ml of 15 mg/ml protein in 200 mM NaCl, 10 mM HEPES, pH 7.5, 5 mM dithiothreitol, 10% glycerol, are mixed with 0.002 ml of reservoir solution containing 0.2 M ammonium sulfate, 26% PEG 4000, 0.1 M sodium cacodylate pH 6.5, 4% w/v benzamidine-HCl, and equilibration against 0.5 ml reservoir solution, 16°C, 2 weeks, method optimization, X-ray diffraction structure determination and analysis at 2.9 A resolution | uncultured bacterium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | E7DJY5 | gene EstF27 from a soil metagenomic library from agricultural soil | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzymefrom Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | uncultured bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.7 | - |
substrate 4-nitrophenyl ferulate, pH and temperature not specified in the publication | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl ferulate + H2O | - |
uncultured bacterium | 4-nitrophenol + ferulate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EstF27 | - |
uncultured bacterium |
FAE | - |
uncultured bacterium |
ferulic acid esterase | - |
uncultured bacterium |